Description of Pyramimonas diskoicola sp. nov. and the importance of the flagellate Pyramimonas (Prasinophyceae) in Greenland sea ice during the winter–spring transition

Harðardóttir, Sara; Lundholm, Nina; Moestrup, Øjvind; Nielsen, Torkel Gissel

doi:10.1007/s00300-014-1538-2

Cited by 21 publications

(14 citation statements)

References 40 publications

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“…For example, Daugbjerg 45 indicated that some Pyramimonas species are able to tolerate a wide range of salinity and Pyramimonas was also reported as a dominant nanoflagellate in surface waters with high freshwater input from the Mackenzie River and surface ice melt in the Beaufort Sea 43 . In addition, Pyramimonas is associated with meltwater pools on sea ice 46 and found during the winter-spring transition in the fjord and ice influenced Disko Bay in Greenland south of our study region 47 .…”

Section: Discussionmentioning

confidence: 48%

Need for focus on microbial species following ice melt and changing freshwater regimes in a Janus Arctic Gateway

Joli

Gosselin

Ardyna

et al. 2018

Sci Rep

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Oceanic gateways are sensitive to climate driven processes. By connecting oceans, they have a global influence on marine biological production and biogeochemical cycles. The furthest north of these gateways is Nares Strait at the top of the North Water between Greenland and Ellesmere Island (Canada). This gateway is globally beneficial, first by supporting high local mammal and bird populations and second with the outflow of phosphate-rich Arctic waters fueling the North Atlantic spring bloom. Both sides of the North Water are hydrologically distinct with counter currents that make this Arctic portal a Janus gateway, after Janus, the Roman god of duality. We examined oceanographic properties and differences in phytoplankton and other protist communities from the eastern and western sides of the North Water (latitude 76.5°N) and found that species differed markedly due to salinity stratification regimes and local hydrography. Typical Arctic communities were associated with south flowing currents along the Canadian side, while potentially noxious Pseudo-nitzschia spp. were dominant on the Greenland side and associated with greater surface freshening from ice melt. This susceptibility of the Greenland side to Pseudo-nitzschia spp. blooms suggest that monitoring species responses to climate mediated changes is needed.

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Section: Discussionmentioning

confidence: 48%

Need for focus on microbial species following ice melt and changing freshwater regimes in a Janus Arctic Gateway

Joli

Gosselin

Ardyna

et al. 2018

Sci Rep

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“…cristatum branches at a different position than the other members of Pyramimonadales in the 16S rDNA tree, making Pyramimonadales polyphyletic. This branching position is inconsistent with previous analyses (Suda, ; Nakayama et al ., ; Viprey et al ., ; Turmel et al ., ; Harðardóttir et al ., ; Jackson et al . ) and may be the result of a tree construction artefact caused by the long branch of Pterosperma .…”

Section: Discussionmentioning

confidence: 99%

“…More recently, Pyramimonadales, specifically genus Pyramimonas , has been revealed as the closest extant relative of euglenophyte plastid by a large‐scale comparative analysis of plastid‐encoded genes (Turmel et al ., ; Jackson et al ., ). Although the diversity of Pyramimonadales has been studied by various means (Nakayama et al ., ; Viprey et al ., ; Turmel et al ., ; Harðardóttir et al ., ; de Vargas et al ., ), it might still hide even closer relatives of euglenid plastids than those known today. The close relationship of Pyramimonas and euglenids, exemplified by Pyramimonas parkeae and Euglena gracilis , is reflected also in the plastid genome organization.…”

Section: Introductionmentioning

confidence: 98%

Analyses of environmental sequences and two regions of chloroplast genomes revealed the presence of new clades of photosynthetic euglenids in marine environments

Lukešová¹,

Karlicki

Hadariová³

et al. 2019

Environ Microbiol Rep

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Euglenophyceae are unicellular algae with the majority of their diversity known from small freshwater reservoirs. Only two dozen species have been described to occur in marine habitats, but their abundance and diversity remain unexplored. Phylogenetic studies revealed marine prasinophyte green alga, Pyramimonas parkeae, as the closest extant relative of the euglenophytes' plastid, but similarly to euglenophytes, our knowledge about the diversity of Pyramimonadales is limited. Here we explored Euglenophyceae and Pyramimonadales phylogenetic diversity in marine environmental samples. We yielded 18S rDNA and plastid 16S rDNA sequences deposited in public repositories and reconstructed Euglenophyceae reference trees. We searched high-throughput environmental sequences from the TARA Oceans expedition and Ocean Sampling Day initiative for 18S rDNA and 16S rDNA, placed them in the phylogenetic context and estimated their relative abundances. To avoid polymerase chain reaction (PCR) bias, we also exploited metagenomic data from the TARA Oceans expedition for the presence of rRNA sequences from these groups. Finally, we targeted these protists in coastal samples by specific PCR amplification of two parts of the plastid genome uniquely shared between euglenids and Pyramimonadales. All approaches revealed previously undetected, but relatively low-abundant lineages of marine Euglenophyceae. Surprisingly, some of those lineages are branching within the freshwater or brackish genera. ; Tel. (+48) 22 55 266 41. † These authors contributed equally to this work.

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“…Regarding the more diverse Arctic nano-phytoplankton, the genus Pyramimonas has often been reported (Lovejoy et al, 2002;Joli et al, 2017) displaying high intra-generic diversity (Daugbjerg and Moestrup, 1993;Balzano et al, 2012) and associated with sea ice (Harðardóttir et al, 2014;Kauko et al, 2018). The mamiellophyte Mantoniella is reported to a lesser extent in Arctic waters (Lovejoy et al, 2007;Terrado et al, 2013;Joli et al, 2017), although diversity within this genus seems to be higher than other polar Mamiellophyceae (Yau et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Culturable diversity of Arctic phytoplankton during pack ice melting

Ribeiro

Santos

Gourvil

et al. 2020

Elementa: Science of the Anthropocene

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Massive phytoplankton blooms develop at the Arctic ice edge, sometimes extending far under the pack ice. An extensive culturing effort was conducted before and during a phytoplankton bloom in Baffin Bay between April and July 2016. Different isolation strategies were applied, including flow cytometry cell sorting, manual single cell pipetting, and serial dilution. Although all three techniques yielded the most common organisms, each technique retrieved specific taxa, highlighting the importance of using several methods to maximize the number and diversity of isolated strains. More than 1,000 cultures were obtained, characterized by 18S rRNA sequencing and optical microscopy, and de-replicated to a subset of 276 strains presented in this work. Strains grouped into 57 phylotypes defined by 100% 18S rRNA sequence similarity. These phylotypes spread across five divisions: Heterokontophyta, Chlorophyta, Cryptophyta, Haptophyta and Dinophyta. Diatoms were the most abundant group (193 strains), mostly represented by the genera Chaetoceros and Attheya. The genera Baffinella and Pyramimonas were the most abundant non-diatom nanoplankton strains, while Micromonas polaris dominated the picoplankton. Diversity at the class level was higher during the peak of the bloom. Potentially new species were isolated, in particular within the genera Navicula, Nitzschia, Coscinodiscus, Thalassiosira, Pyramimonas, Mantoniella and Isochrysis. Culturing efforts such as this one highlight the unexplored eukaryotic plankton diversity in the Arctic and provide a large number of strains for analyzing physiological and metabolic impacts in this changing environment.

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Description of Pyramimonas diskoicola sp. nov. and the importance of the flagellate Pyramimonas (Prasinophyceae) in Greenland sea ice during the winter–spring transition

Cited by 21 publications

References 40 publications

Need for focus on microbial species following ice melt and changing freshwater regimes in a Janus Arctic Gateway

Need for focus on microbial species following ice melt and changing freshwater regimes in a Janus Arctic Gateway

Analyses of environmental sequences and two regions of chloroplast genomes revealed the presence of new clades of photosynthetic euglenids in marine environments

Culturable diversity of Arctic phytoplankton during pack ice melting

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