Description of Pelomonas aquatica sp. nov. and Pelomonas puraquae sp. nov., isolated from industrial and haemodialysis water

Abstract

Three Gram-negative, rod-shaped, non-spore-forming bacteria (strains CCUG 52769T, CCUG 52770 and CCUG 52771) isolated from haemodialysis water were characterized taxonomically, together with five strains isolated from industrial waters (CCUG 52428, CCUG 52507, CCUG 52575T, CCUG 52590 and CCUG 52631). Phylogenetic analysis based on 16S rRNA gene sequences indicated that these isolates belonged to the class Betaproteobacteria and were related to the genus Pelomonas, with 16S rRNA gene sequence similarities higher than 99 % with the only species of the genus, Pelomonas saccharophila and to Pseudomonas sp. DSM 2583. The type strains of Mitsuaria chitosanitabida and Roseateles depolymerans were their closest neighbours (97.9 and 97.3 % 16S rRNA gene sequence similarity, respectively). Phylogenetic analysis was also performed for the internally transcribed spacer region and for three genes [hoxG (hydrogenase), cbbL/cbbM (Rubisco) and nifH (nitrogenase)] relevant for the metabolism of the genus Pelomonas. DNA–DNA hybridization, major fatty acid composition and phenotypical analyses were carried out, which included the type strain of Pelomonas saccharophila obtained from different culture collections (ATCC 15946T, CCUG 32988T, DSM 654T, IAM 14368T and LMG 2256T), as well as M. chitosanitabida IAM 14711T and R. depolymerans CCUG 52219T. Results of DNA–DNA hybridization, physiological and biochemical tests supported the conclusion that strains CCUG 52769, CCUG 52770 and CCUG 52771 represent a homogeneous phylogenetic and genomic group, including strain DSM 2583, clearly differentiated from the industrial water isolates and from the Pelomonas saccharophila type strain. On the basis of phenotypic and genotypic characteristics, these strains belong to two novel species within the genus Pelomonas, for which the names Pelomonas puraquae sp. nov. and Pelomonas aquatica sp. nov. are proposed. The type strains of Pelomonas puraquae sp. nov. and Pelomonas aquatica sp. nov. are CCUG 52769T (=CECT 7234T) and CCUG 52575T (=CECT 7233T), respectively.

The variability in carbon fixation characteristics of several typical chemoautotrophic bacteria at low and high concentrations of CO 2 and its mechanism

“…1, DSM 2625 and DSM 654 possessed only the cbbL gene, DSM 506 contained only the cbbM gene, and DSM 505 comprised both cbbL and cbbM genes. Our results were in accordance with previous studies (Selesi et al, 2005;Dul'tseva et al, 2006;Gomila et al, 2007;Kellermann and Griebler, 2009;Kappler et al, 2012). The expression efficiency and specific catalytic activity of RubisCO are major determining factors of carbon fixation efficiency (Tabita, 1988).…”

“…The order of the carbon fixation yields was DSM 2625 > DSM 505 > DSM 654 > DSM 506. Moreover, the carbon fixation rates of DSM 2625 and DSM 505 also increased vastly at 10% CO 2 when compared with 0.5% CO 2 , and the (Selesi et al, 2005) b-Proteobacteria (Schroeder et al, 2011) facultative autotrophic Pelomona ssaccharophila 654 H 2 cbbL (Gomila et al, 2007) b-Proteobacteria (Xie and Yokota, 2005) facultative autotrophic Sulfur-oxidizing bacteria Thiobacillus thioparus 505 Na 2 S 2 O 3 cbbL (Dul'tseva et al, 2006) and cbbM (Kellermann and Griebler, 2009) b-Proteobacteria (Sattley and Madigan, 2006) obligate autotrophic…”

“…The key active-site residues in the large subunit of forms I and II RubisCO are encoded by the cbbL and cbbM genes, respectively (Kusian and Bowien, 1997). Most hydrogen-oxidizing bacteria use the form I enzyme to fix CO 2 ; they include Hydrogenophaga pseudoflava (Selesi et al, 2005), Hydrogenophilus thermoluteolus (Selesi et al, 2005) and Pelomonas saccharophila (Gomila et al, 2007), while some hydrogen-oxidizing bacteria possess enzymes of both form I and form II, including Hydrogenovibrio marinus (Yoshizawa et al, 2004). Among sulfur-oxidizing bacteria, many species use form II RubisCO to fix CO 2 ; also, research has shown that both forms I and II exist in some Thiobacillius spp., such as Thiobacillus denitrificans (Hernandez et al, 1996), Thiobacillus intermedius (Stoner and Shively, 1993) and Thiobacillus neapolitanus (Baker et al, 1998).…”

Impact of Substratum Surface on Microbial Community Structure and Treatment Performance in Biological Aerated Filters

“…The Pelomonas sp. showed a significant increase in relative abundance in the test reactor during the first reactor start-up but was not detected at all during the second reactor start-up, indicating the bacteria's transient and fortuitous behavior in the process, which was consistent with its infrequent detection in the environment (41).…”

Epiphytic bacterial communities on two common submerged macrophytes in Taihu Lake: diversity and host-specificity

“…Another overlapped genus was Pelomonas within the Betaproteobacteria. This genus contains species previously reported to have nitrogen-fixing ability (Gomila et al, 2007).…”