Description of new genera and species of marine cyanobacteria from the Portuguese Atlantic coast

Brito, Ângela; Ramos, Vítor; Mota, Rita; Lima, Steeve; Santos, Álvaro da Silva; Vieira, Jorge; Vieira, Cristina; Kaštovský, Jan; Vasconcelos, Vı́tor; Tamagnini, Paula

doi:10.1016/j.ympev.2017.03.006

Cited by 57 publications

(58 citation statements)

References 68 publications

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“…One of the largest differences at the species level was observed within the Cyanobacterial group. In particular, high-throughput 16S rRNA revealed a large abundance of S. cyanosphaera, whereas shotgun metagenomic sequencing revealed a more diverse population including members of Pleurocapsa, Myxosarcina and Xenococcus, as previously reported for marine environments [33][34][35][36].…”

Section: Discussionsupporting

confidence: 58%

Microbial communities of the Mediterranean rocky coast: ecology and biotechnological potential

Tanner¹,

Molina‐Menor

Vidal‐Verdú³

et al. 2018

Preprint

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Microbial communities from harsh environments hold great promise as sources of biotechnologically-relevant strains. In the present work, we have deeply characterized the microorganisms from three different rocky locations of the Mediterranean coast, an environment characterised by being subjected to harsh conditions such as high levels of irradiation and large temperature and salinity fluctuations. Through culturedependent and culture-independent techniques, we have retrieved a complete view of the ecology and functional aspects of these communities and assessed the biotechnological potential of the cultivable microorganisms. A culture-independent approach through high-throughput 16S rRNA amplicon sequencing revealed that all three locations display very similar microbial communities, suggesting that there is a stable community associated to the sampled region, with Stanieria cyanosphaera, Rubrobacter sp. and the families Flammeovirgaceae, Phyllobacteriaceae, Rhodobacteraceae and Trueperaceae being the most abundant taxa. Furthermore, shotgun metagenomic sequencing results were in concordance with the highthoughput 16S rRNA, and allowed a description of the eukaryotic and archaeal members of the community, which were abundant in Ascomycota and halotolerant archaea, respectively. The culture-dependent approach yielded a collection of 100 isolates (mainly pigmented), out of which 12 displayed high antioxidant activities, as proved with two in vitro (hydrogen peroxide and DPPH) and an in vivo (model organism C. elegans) assays.

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Section: Discussionsupporting

confidence: 58%

Microbial communities of the Mediterranean rocky coast: ecology and biotechnological potential

Tanner¹,

Molina‐Menor

Vidal‐Verdú³

et al. 2018

Preprint

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“…Interestingly, Jaaginema , one type of filamentous cyanobacterium, was found by the p23S rRNA analysis, and they were abundant from 200 to 6050 m. In general, the genus Jaaginema is mainly present in freshwater ecosystems; however, several species have been identified in marine ecosystems, including Jaaginema litorale ( Brito et al, 2017 ). The species identified from this study corresponded to the previously described marine species Jaaginema litorale .…”

Section: Discussionmentioning

confidence: 99%

Insight Into the Pico- and Nano-Phytoplankton Communities in the Deepest Biosphere, the Mariana Trench

Guo

Liang

et al. 2018

Front. Microbiol.

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As photoautotrophs, phytoplankton are generally present in the euphotic zone of the ocean, however, recently healthy phytoplankton cells were found to be also ubiquitous in the dark deep sea, i.e., at water depths between 2000 and 4000 m. The distributions of phytoplankton communities in much deeper waters, such as the hadal zone, are unclear. In this study, the vertical distribution of the pico- and nano-phytoplankton (PN) communities from the surface to 8320 m, including the epipelagic, mesopelagic, bathypelagic, and hadal zones, were investigated via both 18S and p23S rRNA gene analysis in the Challenger Deep of the Mariana Trench. The results showed that Dinoflagellata, Chrysophyceae, Haptophyta, Chlorophyta, Prochloraceae, Pseudanabaenaceae, Synechococcaceae, and Eustigmatophyceae, etc., were the predominant PN in the Mariana Trench. Redundancy analyses revealed that depth, followed by temperature, was the most important environmental factors correlated with vertical distribution of PN community. In the hadal zone, the PN community structure was considerably different from those in the shallower zones. Some PN communities, e.g., Eustigmatophyceae and Chrysophyceae, which have the heterotrophic characteristics, were sparse in shallower waters, while they were identified with high relative abundance (94.1% and 20.1%, respectively) at the depth of 8320 m. However, the dinoflagellates and Prochloraceae Prochlorococcus were detected throughout the entire water column. We proposed that vertical sinking, heterotrophic metabolism, and/or the transition to resting stage of phytoplankton might contribute to the presence of phytoplankton in the hadal zone. This study provided insight into the PN community in the Mariana Trench, implied the significance of phytoplankton in exporting organic matters from the euphotic to the hadal zone, and also hinted the possible existence of some undetermined energy metabolism (e.g., heterotrophy) of phytoplankton making themselves adapt and survive in the hadal environment.

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“…This is likely explained by the morphology and size of these organisms, as Hyella sp. is a pseudofilamentous, slime-generating and relatively large (10s of µm long colonies) cyanobacterium (Brito et al, 2017), and Cyanothece sp. is a larger unicellular cyanobacterium (≥3 µm diameter) which sometimes also forms aggregates (Reddy et al, 1993).…”

Section: Diazotroph Community Composition In Bulk Samples and Filtratmentioning

confidence: 99%

Filtration via Conventional Glass Fiber Filters in 15N2 Tracer Assays Fails to Capture All Nitrogen-Fixing Prokaryotes

et al. 2018

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Biological dinitrogen fixation (BNF) represents a major input of reduced nitrogen (N) to the oceans. Accurate direct measurements of BNF rates are crucial for reliably determining the biogeochemical significance of diazotrophy at local and global scales. Traditionally, borosilicate glass fiber filters (GF/F, Whatman) with a nominal pore size of 0.7 µm are used to collect suspended particles by filtration after incubations with added 15 N 2 tracer. We carried out BNF experiments in the Baltic Sea, Danish coastal waters, and the Pacific Ocean comparing the retentive characteristics of precombusted GF/F filters with newer Advantec glass fiber filters which have a smaller nominal pore size of 0.3 µm. Where BNF was detected, rates were nearly always higher, and sometimes even exclusively detectable, when using Advantec filters. In the majority of samples across tested habitats, significantly more cells were lost to GF/F filtrate (average = 51%, range = 10-70% of cells) than to Advantec filtrate (average = 40%, range = 10-54%). Using Illumina sequencing of nitrogenase (nifH) gene amplicons, we show that diazotroph communities can markedly differ between bulk water and filtrates from GF/F and Advantec filtrations, suggesting that different diazotrophs can pass through the filter types. In order to reduce the potential underestimations of BNF due to filtration loss of diazotrophs, we recommend using Advantec filters or alternatively silver membranes with 0.2 µm pore size, especially in waters expected to be inhabited by relatively small, unicellular diazotrophs.

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Description of new genera and species of marine cyanobacteria from the Portuguese Atlantic coast

Cited by 57 publications

References 68 publications

Microbial communities of the Mediterranean rocky coast: ecology and biotechnological potential

Microbial communities of the Mediterranean rocky coast: ecology and biotechnological potential

Insight Into the Pico- and Nano-Phytoplankton Communities in the Deepest Biosphere, the Mariana Trench

Filtration via Conventional Glass Fiber Filters in 15N2 Tracer Assays Fails to Capture All Nitrogen-Fixing Prokaryotes

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