Description of Mycobacterium tuberculosis mutations conferring resistance to rifampicin and isoniazid detected by GenoType® MTBDRplus V.2 in Colombia

Llerena, Claudia; Medina, Raquel

doi:10.7705/biomedica.v37i1.3174

Cited by 5 publications

(5 citation statements)

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“…Table 4 shows that INH mutations primarily occur at katG 315 and in the inhA promoter at − 15. For katG 315, AGC to ACC was the main mutation of this residue in our study, with 100% of cases showing the katG Ser315Thr AGC to ACC mutation, and we found no Ser315Asn AGC to AAC mutations, which was consistent with previous reports [ 21 , 33 ]. However, in our study, inhA − 15 locus mutations accounted for 46.4% of all INH resistance mutations, which was inconsistent with previous reports [ 34 – 36 ].…”

Section: Discussionsupporting

confidence: 93%

GeneChip analysis of resistant Mycobacterium tuberculosis with previously treated tuberculosis in Changchun

Zhang

Ren

Sun³

et al. 2018

BMC Infect Dis

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BackgroundWith the widespread use of rifampicin and isoniazid, bacterial resistance has become a growing problem. Additionally, the lack of relevant baseline information for the frequency of drug-resistant tuberculosis (TB) gene mutations is a critical issue, and the incidence of this infection in the city of Changchun has not investigated to date. However, compared with the slow traditional methods of drug susceptibility testing, recently developed detection methods, such as rifampicin and isoniazid resistance-related gene chip techniques, allow for rapid, easy detection and simultaneous testing for mutation frequency and drug resistance.MethodsIn this study, the rifampicin and isoniazid resistance-related gene mutation chip method was employed for an epidemiological investigation. To assess the gene mutation characteristics of drug-resistant TB and evaluate the chip method, we tested 2143 clinical specimens from patients from the infectious diseases hospital of Changchun city from January to December 2016. The drug sensitivity test method was used as the reference standard.ResultsThe following mutation frequencies of sites in the rifampicin resistance gene rpoB were found: Ser531Leu (52.6%), His526Tyr (12.3%), and Leu511Pro (8.8%). The multidrug-resistance (MDR)-TB mutation frequency was 34.7% for rpoB Ser531Leu and katG Ser315Thr, 26.4% for rpoB Ser531Leu and inhA promoter − 15 (C → T), and 10.7% for rpoB His526Tyr and katG Ser315Thr. In addition, drug susceptibility testing served as a reference standard. In previously treated clinical cases, the sensitivity and specificity of GeneChip were 83.1 and 98.7% for rifampicin resistance, 79.9 and 99.6% for isoniazid resistance, and 74.1 and 99.8% for MDR-TB.ConclusionsOur experimental results show that the chip method is accurate and reliable; it can be used to detect the type of drug-resistant gene mutation in clinical specimens. Moreover, this study can be used as a reference for future research on TB resistance baselines.Electronic supplementary materialThe online version of this article (10.1186/s12879-018-3131-8) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 93%

GeneChip analysis of resistant Mycobacterium tuberculosis with previously treated tuberculosis in Changchun

Zhang

Ren

Sun³

et al. 2018

BMC Infect Dis

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“…Several recent studies have examined the contribution of katG and inhA promoter mutations in drug-resistant TB isolates, and the results revealed significant geographic diversity across regions [ 25 – 27 ]. This study found that the most common mutation of MDR-TB was 34.7% (Ser531 → Leu + Ser315 → Thr), however, a study in Brazil showed that was 41.7% [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Prevalence and molecular characteristics of drug-resistant Mycobacterium tuberculosis in Hainan, China: from 2014 to 2019

Lin

Zhao

et al. 2021

BMC Microbiol

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Background The emergence of antimicrobial resistance against Mycobacterium tuberculosis (M. tuberculosis) has become the major concern in global tuberculosis control due to its limited therapy options and high mortality. However, the clinical and molecular characteristics of drug-resistant strains vary in different geographical areas. Hainan Island located in southern China, is a high drug-resistant tuberculosis burden area. This study aimed to determine the dynamic changes of drug-resistance patterns and drug-related gene mutation types of M. tuberculosis in Hainan from 2014 to 2019. Results A total of 1484 culture-confirmed M. tuberculosis were included in this study. It was found that the proportions of drug resistance to isoniazid and rifampin were 31.3 and 31.1% respectively. Overall the proportion of multidrug resistant M. tuberculosis was 24.9%. Multivariate logistic regression analysis showed that age and the treatment history were independent influencing factors of drug resistant tuberculosis. The proportions of drug-resistant tuberculosis in retreatment patients were considerably higher than those in new patients. The most common mutation types of isoniazid were Ser315 → Thr (66.3%), and the most common mutation types of rifampin were Ser531 → Leu (41.5%). Conclusions Our data suggests that the prevalence of drug resistant TB remains high in Hainan, and the risks for developing drug resistance with diversified mutation types increased significantly in retreatment patients. These results contribute to the knowledge of the prevalence of drug resistance in Hainan Province and expand the molecular characteristics of drug resistance in China simultaneously.

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“…A total of 1,664 studies were discovered through electronic databases (PubMed, EMBASE, Google Scholar, and Science Direct) and other searches (such as organizational records and websites). Approximately 1,115 studies were excluded due to duplication, 351 studies were excluded due to differences in study setting/context (21)(22)(23)(24)(25)(26)(27)(28)(29), 105 studies were excluded due to differences in outcome interest (30)(31)(32)(33), and 77 studies were excluded due to differences in the study population (27)(28)(29). Finally, 16 cross-sectional studies were identified as potentially relevant for inclusion in the current meta-analysis and systematic review (Figure 1).…”

Section: Search Resultsmentioning

confidence: 99%

Molecular detection of rifampicin-resistant Mycobacterium tuberculosis by polymerase chain reaction in Ethiopia: a systematic review and meta-analysis

Birhanu,

Bekele,

Jemberie

2024

Front. Med.

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IntroductionTuberculosis is a contagious bacterial disease caused by Mycobacterium tuberculosis. The emergence and spread of drug-resistant strains of M. tuberculosis in both developing and developed countries has made diagnosis, treatment, and control of tuberculosis more difficult. The PCR assay, which is a fast and sensitive technique and an alternative method for detecting multidrug-resistant tuberculosis, is used to determine rifampicin (RIF) resistance. There is no single figure in Ethiopia that represents rifampicin-resistant tuberculosis and that is why this study was conducted to overcome the inconsistency of the results of the previous studies.MethodsStudies were researched from five major electronic databases. Studies which were cross-sectional in design, published, and written in English were included. The data were extracted using Microsoft Excel, and the data were managed and analyzed using Stata™ Version 17.0 statistical software. The Forest plot was used to check the presence of heterogeneity. The publication bias, meta-regression, and subgroup analysis were used to find out the source of heterogeneity. A random effect analysis model was used to pool the prevalence of RR TB from primary studies, and associated factors of RR among TB patients were identified using Meta regression. The presence of association was reported using OR with 95% CI.ResultsThe overall pooled prevalence of tuberculosis was 14.9% (95% CI: 13.34, 16.46), of these approximately 7.48% (95% CI: 6.30, 8.66) showed rifampicin-resistant tuberculosis in Ethiopia. Among the computed variables, 2.05% living with HIV1.39 (95%CI: 1.13, 1.72) and having a history of TB treatment (95%CI: 1.34, 3.15) were identified as significant factors associated with RR TB in Ethiopia.ConclusionDrug-resistant TB is one of the prevalent emerging infectious diseases among TB patients, which affects approximately one out of every thirteen TB patients. Having TB-HIV coinfection and a history of prior TB treatment were identified as significant factors associated with RR TB. To prevent and control RR TB, patients should complete their follow-up course; the health professionals should educate the actions taken by the patients when they experience drug toxicity and side effects; and the Minister of Health should initiate telemedicine and recruit tracers to overcome TB patients’ default and have good drug adherence and retention after initiation of the treatment.

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Description of Mycobacterium tuberculosis mutations conferring resistance to rifampicin and isoniazid detected by GenoType® MTBDRplus V.2 in Colombia

Cited by 5 publications

References 5 publications

GeneChip analysis of resistant Mycobacterium tuberculosis with previously treated tuberculosis in Changchun

GeneChip analysis of resistant Mycobacterium tuberculosis with previously treated tuberculosis in Changchun

Prevalence and molecular characteristics of drug-resistant Mycobacterium tuberculosis in Hainan, China: from 2014 to 2019

Molecular detection of rifampicin-resistant Mycobacterium tuberculosis by polymerase chain reaction in Ethiopia: a systematic review and meta-analysis

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