Description of Mariniphaga anaerophila gen. nov., sp. nov., a facultatively aerobic marine bacterium isolated from tidal flat sediment, reclassification of the Draconibacteriaceae as a later heterotypic synonym of the Prolixibacteraceae and description of the family Marinifilaceae fam. nov.

Abstract

A mesophilic, chemoheterotrophic bacterium, strain Fu11-5T, was isolated from tidal-flat sediment from Tokyo Bay, Chiba, Japan. Cells of strain Fu11-5T were facultatively aerobic, Gram-negative, non-sporulating, non-motile and rod-shaped (1.9–6.9 µm long). Strain Fu11-5T grew optimally at 35–37 °C and pH 6.5–7.0 and with 1–2 % (w/v) NaCl. Oxygen and l-cysteine were used as an alternative electron acceptor and donor, respectively. Strain Fu11-5T also grew fermentatively on some pentoses, hexoses and disaccharides and soluble starch. Succinic acid was the major end product from d-glucose. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain Fu11-5T was affiliated with the order Bacteroidales , and its nearest neighbours were members of the genera Meniscus , Prolixibacter , Sunxiuqinia , Mangrovibacterium and Draconibacterium, with 87–91 % sequence similarity. Cell morphology, optimum growth temperature and utilization of sugars of strain Fu11-5T distinguished the strain from phylogenetically related bacteria. On the basis of its phenotypic features and phylogenetic position, a novel genus and species are proposed to accommodate strain Fu11-5T, with the name Mariniphaga anaerophila gen. nov., sp. nov. The type strain of Mariniphaga anaerophila is strain Fu11-5T ( = JCM 18693T = NBRC 109408T = DSM 26910T). We also propose to combine the family Draconibacteriaceae into the family Prolixibacteraceae as a later heterotypic synonym and to place the distinct sublineage of the genus Marinifilum in the family Marinifilaceae fam. nov.

Distribution and Evolution of Nitrogen Fixation Genes in the Phylum <i>Bacteroidetes</i>

“…All these species belong to the order Bacteroidales (class Bacteroidia ), and are distributed in three families, Marinilabiliaceae ( Saccharicrinis , Geofilum , and Alkaliflexus ), Porphyromonadaceae ( Dysgonomonas and Paludibacter ), and Bacteroidaceae ( Bacteroides ), among the six recently updated families within Bacteroidales (20). The gene encoding a homologous protein to conventional nitrogenases was not detected in the genome sequences of strains in the other classes in Bacteroidetes ( Cytophagia , Flavobacteriia , and Sphingobacteriia ), although they accounted for 42.6% of the searched genomes.…”

Puteibacter caeruleilacunae gen. nov., sp. nov., a facultatively anaerobic bacterium isolated from Yongle Blue Hole in the South China Sea

“…Draconibacterium [2], Mangrovibacterium [1], Mariniphaga [3], Meniscus [4], Prolixibacter [5], Sunxiuqinia [6], Tangfeifania [7] and Roseimarinus [8]. All members of the family Prolixibacteraceae are Gram-negative bacteria and facultative anaerobes that use menaquinone-7 (MK-7) as the main respiratory quinone.…”

Mangrovibacterium lignilyticum sp. nov., a facultatively anaerobic lignin-degrading bacterium isolated from mangrove sediment

“…[7]. At the time of writing, the family Prolixibacteraceae included nine genera: Prolixibacter [8], Mangrovibacterium [6], Meniscus [9], Mariniphage [10], Sunxiuqinia [11], Draconibacterium [12], Roseimarinus [13], Tangfeifania [14] and Maribellus [15].…”