Description of Maribacter forsetii sp. nov., a marine Flavobacteriaceae isolated from North Sea water, and emended description of the genus Maribacter

Abstract

This is an author manuscript that has been accepted for publication in International Journal of Systematic and Evolutionary Microbiology, copyright Society for General Microbiology, but has not been copy-edited, formatted or proofed. Cite this article as appearing in International Journal of Systematic and Evolutionary Microbiology. This version of the manuscript may not be duplicated or reproduced, other than for personal use or within the rule of 'Fair Use of Copyrighted Materials' (section 17, Title 17, US Code), without permission from the copyright owner, Society for General Microbiology. The Society for General Microbiology disclaims any responsibility or liability for errors or omissions in this version of the manuscript or in any version derived from it by any other parties. The final copy-edited, published article, which is the version of record, can be found at http://mic.sgmjournals.org, and is freely available without a subscription.International audienceThree rod-shaped, Gram-negative, chemo-organotrophic, heterotrophic, strictly aerobic, gliding bacterial strains, KT02ds18-4, KT02ds18-5 and KT02ds18-6T, were isolated from North Sea surface waters near the island of Helgoland, Germany. Their taxonomic position was investigated by a polyphasic approach. The three strains were light yellow, oxidase- and catalase-positive, and grew optimally at 25 degrees C, at pH 7.5, and in the presence of 2.5 % (w/v) NaCl. The Chargaff's coefficient was 34.2-34.4 mol%. The three strains shared >90 % DNA-DNA relatedness and an identical 16S rRNA gene sequence. Comparative 16S rRNA gene sequence analysis allocated the three strains to the genus Maribacter in the family Flavobacteriaceae, with similarities of 97.0-97.4 % to five of the recognized Maribacter species. Their low level of DNA-DNA relatedness (<20 %) with these species and differentiating phenotypic characteristics demonstrated that they constitute a new Maribacter species for which the name Maribacter forsetii sp. nov. is proposed. Strain KT02ds18-6T (=CIP 109504T=DSM 18668T) is the type strain. An emended description of the genus Maribacter is also proposed

Diversity and Antagonistic Activity of Sea Ice Bacteria Isolated from the Sea of Japan

“…These species were primarily isolated from a sea water sample collected from Peter the Great Bay during the summer season 24) . Maribacter strains recently isolated from North Sea waters were proposed as Maribacter forsetii 3) . An abundance of Bacteroidetes members, including bacteria of the genera Gelidibacter, Psychroserpens, and Psychroflexus 7,11) have been isolated from Antarctic sea ice and sea water.…”

Maribacter luteus sp. nov., a marine bacterium isolated from intertidal sand of the Yellow Sea

“…At the time of writing, the genus comprises 23 species with validly published names. All members of the genus Maribacter were isolated from diverse marine environments, such as seawater [2,3], marine sediment [4], red alga [5], seaweed [6] and sponge [7]. The genus Maribacter is generally characterized to be Gram-stain-negative, aerobic or facultatively anaerobic, motile by gliding or non-motile.…”

“…The major or only respiratory quinone is menaquinone 6 (MK-6), and the major fatty acids are straight-chain saturated, branched-chain saturated and unsaturated fatty acids iso-C 15 : 0 , iso-C 15 : 1 , C 15 : 0 and iso-C 17 : 0 3-OH. The genomic DNA G+C contents are in the range of 32.6-41.1 % [1,2,6]. In this study, a novel bacterial strain, designated RZ05 T , was isolated and determined using a polyphasic taxonomic approach.…”

Morphogenesis of Ulva mutabilis (Chlorophyta) induced by Maribacter species (Bacteroidetes, Flavobacteriaceae)

“…Maribacter are gram-negative, rod shaped cells, which produce non-diffusible yellow to orange pigments. The metabolism is aerobic or facultatively anaerobic (Nedashkovskaya et al 2004a, Barbeyron et al 2008, Lo et al 2013, Weerawongwiwat et al 2013, Hu et al 2015, Jackson et al 2015. They were found in various marine sources and climatic zones (Cho et al 2008, Zhang et al 2009, Tang et al 2015 and could be isolated from the water column (Yoon et al 2005, Barbeyron et al 2008) and the sea sediment (Nedashkovskaya et al 2004a, Cho et al 2008.…”

“…The metabolism is aerobic or facultatively anaerobic (Nedashkovskaya et al 2004a, Barbeyron et al 2008, Lo et al 2013, Weerawongwiwat et al 2013, Hu et al 2015, Jackson et al 2015. They were found in various marine sources and climatic zones (Cho et al 2008, Zhang et al 2009, Tang et al 2015 and could be isolated from the water column (Yoon et al 2005, Barbeyron et al 2008) and the sea sediment (Nedashkovskaya et al 2004a, Cho et al 2008. Maribacter strains are often associated with the surface of macroalgae such as Ulva fenestra Postels & Ruprecht (Nedashkovskaya et al 2004a, Weerawongwiwat et al 2013, U. mutabilis (Spoerner et al 2012) and Polysiphonia (Nedashkovskaya et al 2007), where they harbour various enzyme activities involved in degradation of, for example, algal polysaccharides (Bakunina et al 2012).…”