Description of Leishmania species among dogs and humans in Colombian Visceral Leishmaniasis outbreaks

Herrera, Giovanny; Higuera, Adriana; Patiño, Luz Helena; Ayala, Martha S.; Ramírez, Juan David

doi:10.1016/j.meegid.2018.06.023

Cited by 11 publications

(9 citation statements)

References 18 publications

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“…The findings reinforce the establishment of surveillance and control programs in other rural and urban regions of Colombia, where favorable eco-epidemiological conditions exist (Arbelaez et al, 2020), and also the need for seroprevalence studies (Cortes, 2006). With the current report in Risaralda, in addition to Valle del Cauca, Caldas, and Putumayo (Figure 1), there are four new departments (Herrera et al, 2018) that have reported Le. infantum before non-endemic since 2018, highlighting the need for more research on VL in Colombia.…”

Section: Discussionsupporting

confidence: 75%

An Urban Case of Canine Visceral Leishmaniasis in the Municipality of Pereira, Colombia

González-Colonia¹,

Bonilla-Aldana²,

Álvarez-Serrano³

et al. 2021

WVJ

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American visceral leishmaniasis (VL) is caused by Leishmania infantum/chagasi, transmitted by the sandflies Lutzomyia longipalpis and Lu. evansi. Dogs are the main reservoir and source for zoonotic infections in humans. Therefore, it is of utmost importance to diagnose such diseases in domestic animals to maintain public health. In 2019, the authors of the present study observed intracellular amastigotes in Giemsa-stained bone marrow smear using a real-time qPCR (parasite load was 484,336 DNA copies/mL), a canine visceral leishmaniasis (CVL) case caused by L. infantum/chagasi, in a 22-month-old male, English bulldog from Pereira, Colombia, a municipality not previously considered endemic for CVL nor human VL. Therefore, further field studies are necessary to determine if there is a low-grade circulation of this parasite among dogs and other animal reservoirs in the area, also entomological surveys are of utmost importance, as well as highlighting the clinical suspicion of this disease in domestic animals and humans.

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Section: Discussionsupporting

confidence: 75%

An Urban Case of Canine Visceral Leishmaniasis in the Municipality of Pereira, Colombia

González-Colonia¹,

Bonilla-Aldana²,

Álvarez-Serrano³

et al. 2021

WVJ

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“…By employing the sensitive and specific ITS-rDNA gene (36), approximately 30% of the sampled dogs were found to be infected with L. infantum (n = 10), as the most important recognized causative agent of visceral leishmaniasis in dogs (23,43,44), as well as L. tropica (n = 1). Nearly 50% of the infected specimens were found in conjunctival samples, which were significantly more infected than blood samples.…”

Section: Discussionmentioning

confidence: 99%

“…Molecular methods, unlike serological tools, can firmly discriminate Leishmania species. Sensitivity and specificity of molecular tools have been assessed in visceral leishmaniasis diagnosis in both canines and humans with variable values (21)(22)(23). The specificity of molecular tests is often 95% to 100% and its sensitivity can be between 0.001 and 0.1 parasite/reaction (7,24,25).…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Visceral Leishmaniasis in Asymptomatic Dogs in North Khorasan, Northeastern Iran

Taslimian

Shemshadi

Spotin

et al. 2020

Jundishapur J Microbiol

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Background: Leishmania infantum parasites are the main causative agents of visceral leishmaniasis that threaten a wide range of humans and canines in Iran. Objectives: Our aim was to survey Leishmania parasite species and simultaneous comparison of canine organs in endemic areas for the diagnosis of visceral leishmaniasis using the ITS-rDNA gene, sequencing, and phylogenetic analysis. Methods: In this study, sampling was done with vacuum tubes containing EDTA from blood and sterile swabs from the snout and conjunctiva of asymptomatic sheepdogs (n = 37) using a non-invasive method in north Khorasan, northeastern Iran, from 28 July to 4 August 2018. The DNA of collected samples was extracted, amplified, and sequenced by targeting the ITS-rDNA gene. To demonstrate the taxonomic status of Leishmania spp., sequences were subjected to phylogenetic analysis based on the maximum likelihood method. Results: We obtained 37 samples from asymptomatic dogs of which, 10 dogs were definitely diagnosed with L. infantum and one dog infected with L. tropica. The blood (n = 8) and right conjunctiva (n = 6) samples were the most infected samples. The highest number of infections in dogs was in the age group of 5-10 years indicating that this group is more sensitive to visceral leishmaniasis in this region. Conclusions: The current findings indicate that non-invasive sampling and molecular methods are reliable and suitable in the detection of visceral leishmaniasis. This is the first report of the visceral involvement of a shepherd dog with L. tropica in northeastern Iran. The remarkable occurrence of visceral leishmaniasis (29.7%) in asymptomatic sheepdogs reflects a health alert to conduct the surveillance and monitoring of susceptible individuals/reservoirs in the region.

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“…More recently PCR coupled to High Resolution Melting (HRM) genotyping analysis [21][22][23] have also been implemented. Some additional reports of Leishmania identi cation have showed the use of direct Sanger sequence analysis of PCR-ampli ed genes, like the hsp70 [24,25], or minicircle kDNA [26], among others.…”

Section: Introductionmentioning

confidence: 99%

An Updated Geographical Distribution of Leishmania Species Involved in Human Leishmaniasis in Colombia

Vélez

Muskus

Robledo

2021

Preprint

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Leishmaniasis in an endemic disease in Colombia. This country ranks second in number of cases of leishmaniasis in Latin America and is one of the four countries with the largest number of Leishmania species. Although leishmaniasis is a notifiable disease, the information about the geographic distribution of Leishmania species circulating in the country is fragmented. Since 1990, when the first distribution map was published in Colombia, very few new reports have described the Leishmania species present in Colombia and their geographic distribution. In this report we included 1,833 natural isolates obtained from human cases of leishmaniasis. Their geographical origin together with those reported by other authors let us confirm the presence of Leishmania species in 30 of 32 Colombian departments, being Quindío and San Andres/Providencia free of transmission. In this work, L. braziliensis is described for first time in the departments of Atlántico, Caldas and Vaupes and L. panamensis in the departments of Guajira and Vaupés. It is also confirmed that L. panamensis and L. braziliensis coexist in the same regions being the main causative agents of cutaneous leishmaniasis in Colombia; nonetheless, L. panamensis predominates in the north and western regions whereas L. braziliensis predominates in the southeast region.

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Description of Leishmania species among dogs and humans in Colombian Visceral Leishmaniasis outbreaks

Cited by 11 publications

References 18 publications

An Urban Case of Canine Visceral Leishmaniasis in the Municipality of Pereira, Colombia

An Urban Case of Canine Visceral Leishmaniasis in the Municipality of Pereira, Colombia

Molecular Characterization of Visceral Leishmaniasis in Asymptomatic Dogs in North Khorasan, Northeastern Iran

An Updated Geographical Distribution of Leishmania Species Involved in Human Leishmaniasis in Colombia

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