Description of <i>Desmonostoc caucasicum sp. nov</i>. (Cyanobacteria) using an integrative taxonomic approach

Maltseva, Svetlana; Bachura, Yuliya; Erst, Tatyana V.; Kulikovskiy, Maxim; Maltsev, Yevhen

doi:10.1080/00318884.2022.2086428

Cited by 5 publications

(11 citation statements)

References 38 publications

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“…are the most common cyanobiont representatives but species of Calothrix, Scytonema and Richelia have also been reported. [14][15][16] Nostoc sp. is also the most commonly reported cyanobiont from lichens, but other intriguing genera have also been reported, such as Rhizonema, capable of unicellular, falseand true-branching morphologies (Fig.…”

Section: Introductionmentioning

confidence: 99%

Highlights of biosynthetic enzymes and natural products from symbiotic cyanobacteria

D’Agostino

2023

Nat. Prod. Rep.

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Section: Introductionmentioning

confidence: 99%

Highlights of biosynthetic enzymes and natural products from symbiotic cyanobacteria

D’Agostino

2023

Nat. Prod. Rep.

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“…For example, Odorella benthonica shows 99.92% similarity to Pleurocapsa minor (JQ070059) and is separated by distinct morphology and ecological differences [54], as cited in [55]. In the heterocystous Nostocaceaen group, Desmonostoc muscorum Lukesova 1/87 shows 99.3% 16S rRNA gene sequence similarity to D. geniculatum HA4340-LM1 [21], and D. magnisporum AR6-PS shows 99.1% similarity to D. punense MCC 2741, as both shown in [34,35]. In this circumstance, the p-distance method based on the 16S rRNA gene cannot be used as the only absolute basis for separating terminal taxonomic levels (i.e., genera and species).…”

Section: Discussionmentioning

confidence: 99%

“…Related ecological factors encompass the concept of cyanobacterial taxonomic descriptions and can be considered as an extension of the polyphasic approach [20,55]. In addition, the use of other molecular markers that have been utilized in previous studies-especially for groups with cryptic traits-could help in the assessment of phylogenetic relationships at the cyanobacterial genus and species levels [35,37]. In this study, the rbcL gene was used as an additional genetic marker for characterizing S. nagquensis sp.…”

Section: Discussionmentioning

confidence: 99%

“…The rbcL gene was additionally amplified, using the primers rbcLf (5 -GACTTCACCAAAGAYGACGAAAACAT-3 ) and rbcLr (5 -GAACTCGAACTTRATYTCTTTCCA-3 ). These primer regions were adapted from [13,[34][35][36][37]. The PCR conditions used for the amplification of the primers mentioned above are provided in Supplementary Table S1 [13,[34][35][36][37].…”

Section: Dna Extraction Pcr Amplification and Gene Sequencingmentioning

confidence: 99%

“…These primer regions were adapted from [13,[34][35][36][37]. The PCR conditions used for the amplification of the primers mentioned above are provided in Supplementary Table S1 [13,[34][35][36][37]. All PCR reactions were performed in 25 µL volumes containing equal volumes of purified water and PCR FastTaq Premix (TOLO Biotech Co., Ltd., Shanghai, China), 1 µL of each primer (0.01 mM concentration), and 1.5 µL of template DNA (20 ng µL −1 ).…”

Section: Dna Extraction Pcr Amplification and Gene Sequencingmentioning

confidence: 99%

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Stenomitos nagquensis sp. nov. (Leptolyngbyaceae, Cyanobacteria) from a Meadow Wetland in the Tibet Plateau: A Novel Species Studied Based on a Polyphasic Approach

et al. 2023

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A novel cyanobacterial strain (CSML-F035) here described as Stenomitos nagquensis sp. nov. was isolated from a plateau meadow wetland habitat in the Tibet Autonomous Region. The novel strain was studied based on a polyphasic approach by combining characteristics of cell morphology and evidence from molecular data and ecology. Phylogenetic analyses based on the 16S rRNA and rbcL genes indicated that the novel strain is a member of the genus Stenomitos (Leptolyngbyaceae) but is positioned in a unique clade, with strongly supported values from BI, ML, and NJ methods. The percentage similarity of the 16S rRNA gene sequence of CSML-F035 compared with known strains of Stenomitos was >97%. Morphologically, S. nagquensis sp. nov. possesses overlapping morphological characteristics with other Stenomitos species, with thin trichomes enclosed in a firm, colorless, hyaline, persistent mucilage. However, the strain appeared to have slightly smaller cells compared to most known species of Stenomitos. Further analyses of the 16S–23S ITS region showed dissimilarity at 11.2–22.8% compared to known species of Stenomitos. The secondary helices—including D1–D1, V2, Box–B, and V3—that were predicted based on the 16S–23S ITS region for Stenomitos nagquensis sp. nov. were also distinct, thereby providing strong additional support for the separation of this species. This study highlights the importance of unexplored habitats in the Tibet region in which cyanobacterial diversity can be deemed high, with many potential new species that remain poorly explored.

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Discovery of Cyanobacteria Associated with Cycads and Description of Three Novel Species in Desmonostoc (Nostocaceae)

Pecundo,

Chen,

dela Cruz

et al. 2023

Diversity

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Heterocyte-forming cyanobacteria form symbiotic relationships with several lineages of plants. Here, twenty (20) strains of endosymbiotic cyanobacteria (cyanobionts) with Nostoc-like morphologies were isolated from the highly specialized coralloid roots of five host species in Cycadales—Cycas debaoensis, C. fairylakea, C. elongata, Ceratozamia robusta, and Macrozamia moorei. Molecular phylogeny based on the 16S rRNA gene placed these strains into seven different taxa within the Nostocaceae, specifically under the genera Desmonostoc and Dendronalium. The percent dissimilarity and unique patterns in the secondary structures of the D1-D1′, Box-B, V2, and V3 helices, which were based on the 16S–23S rRNA internal transcribed spacer (ITS) regions, supported three distinct species in Desmonostoc. These three morphologically distinct novel species are described in this report: Desmonostoc debaoense sp. nov., Desmonostoc meilinense sp. nov., and Desmonostoc xianhuense sp. nov. Other investigated strains were phylogenetically identified as members of the recently discovered genus Dendronalium and represent the first report of association of that genus with cycads. Our findings suggest that the order Cycadales hosts diverse species of cyanobionts in their coralloid roots and that many potential unreported or novel taxa are present in cycads occurring in their natural habitat and await discovery.

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Description of Desmonostoc caucasicum sp. nov. (Cyanobacteria) using an integrative taxonomic approach

Cited by 5 publications

References 38 publications

Highlights of biosynthetic enzymes and natural products from symbiotic cyanobacteria

Highlights of biosynthetic enzymes and natural products from symbiotic cyanobacteria

Stenomitos nagquensis sp. nov. (Leptolyngbyaceae, Cyanobacteria) from a Meadow Wetland in the Tibet Plateau: A Novel Species Studied Based on a Polyphasic Approach

Discovery of Cyanobacteria Associated with Cycads and Description of Three Novel Species in Desmonostoc (Nostocaceae)

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