Description of dogs naturally infected with Hepatozoon canis in the Aegean region of Turkey

Paşa, Serdar; Kiral, Funda; Karagenç, Tülin; Atasoy, Abidin; Seyrek, Kamil

doi:10.3906/vet-0801-11

Cited by 11 publications

(12 citation statements)

References 25 publications

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“…It has been reported that the disease can be seen in both sexes (Gavazza et al 2003). Some researchers (Paşa et al 2009) reported that females are more sensitive than males, while others (Gomes et al 2010;Aktas et al 2015;Tuna et al 2020) reported that males are more sensitive than females. In these studies, it is reported that there is no significant difference between the sexes (Paşa et al 2009;Gomes et al 2010;Aktas et al 2015;Tuna et al 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Some researchers (Paşa et al 2009) reported that females are more sensitive than males, while others (Gomes et al 2010;Aktas et al 2015;Tuna et al 2020) reported that males are more sensitive than females. In these studies, it is reported that there is no significant difference between the sexes (Paşa et al 2009;Gomes et al 2010;Aktas et al 2015;Tuna et al 2020). The positivity obtained in this study was found to be higher in males (11.11%) compared to females (7.69%), and no significant difference was detected (P > 0.05).…”

Section: Discussionmentioning

confidence: 99%

“…Canine hepatozoonosis is a chronic disease that can infect dogs of all ages. In the study carried out by Paşa et al (2009), H. canis infected dogs were reported to be between the ages of 2 and 8 years. In a study by Baneth and Weigler (1997), it is reported that the H. canis infection is most common in dogs younger than 6 months and dogs aged 5-10 years.…”

Section: Discussionmentioning

confidence: 99%

“…Hepatozoon canis infection can range from an asymptomatic condition in dogs with low parasitaemia rates to a serious life-threatening illness with fever, lethargy, anaemia, and emaciation in dogs with high parasitaemia (Baneth et al 2003;Gonen et al 2004;Baneth 2011). Clinical signs of H. canis infection vary depending on the age of the host, the degree of infection, and the presence of concomitant infections, although fever, lethargy, and weight loss are among the most prominent symptoms (Paşa et al 2009;Altay et al 2013;Aktas et al 2015).…”

mentioning

confidence: 99%

“…Microscopic examination (Elias and Homans 1988;Paşa et al 2009;Baneth 2011), serological (IFAT, ELISA) (Baneth et al 1996;Gonen et al 2004;Karagenc et al 2006;Baneth 2011) and molecular methods (Inokuma et al 2002;Rubini et al 2005;O'Dwyer 2011) are used in the diagnosis of the disease. It is reported that molecular methods are more sensitive and specific than microscopic and serological methods in the diagnosis of hepatozoonosis and other blood parasites (Altay et al 2019).…”

mentioning

confidence: 99%

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A Molecular survey of Hepatozoon canis in dogs in the Siirt province of Turkey

Çelik

Ayan

et al. 2022

Acta Vet. Brno

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This study aimed to determine Hepatozoon canis prevalence in dogs in the Siirt province of Turkey by the molecular method. The animal material of the study consisted of a total of 75 dogs that appeared clinically healthy. Two ml of blood sample were taken from the vena cephalica antebrachii. Then, DNA extraction was performed. Polymerase chain reaction (PCR) was performed to amplify the 666 bp 18S rRNA gene region of Hepatozoon canis. Two positive PCR products were purified and sequenced. As a result of Nested-PCR, H. canis specific bands in 666 bp size were obtained in 7 (9.33%) out of 75 dogs. The result of sequence analysis, the nucleotide sequence was registered in the NCBI GenBank database with accession numbers OL467380.1-OL467538.1. Hepatozoon canis registered in GenBank of sequence OL467380.1 was found to be similar with other H. canis strains of registration numbers MW684292.1 with 99.69% and MH615006.1-MK091085.1-MF797806.1 with 99.53% rates; and the sequence with registration number OL467538.1 was found to be similar to the series MW684291.1 with 99.09% and MH615006.1-MK091085.1-KX 818220.1 with 99.08% rates by BLAST analysis. Hepatozoon canis prevalence of dogs in the Siirt province was determined as a result of this study. It is of great importance to take preventive measures, especially to fight ticks with appropriate acaricides, since there is no vaccine to prevent the disease.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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A Molecular survey of Hepatozoon canis in dogs in the Siirt province of Turkey

Çelik

Ayan

et al. 2022

Acta Vet. Brno

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Köpeklerde kene kaynaklı bazı protozoon ve rickettsial enfeksiyonların Real Time PCR ile araştırılması ve saptanan izolatların moleküler karakterizasyonları

Önder; İNCİ

2014

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Özet: Bu canis vogeli ve B. gibsoni izolatlarının 18S rRNA gen bölgesine ait sekanslarının dünyadaki aynı türden diğer izolatlarla ikili kıyaslamalarında %1,4±0,2, %0,3±0,2 ve %0,9±0,3 genetik farklılık saptanmıştır. E. canis için 16S rRNA gen bölgesi yönünden üç izolatın kendi aralarında %100 identik olduğu, dünyadaki izolatlarla ise %0,1 farklılık gösterdiği; A. phagocytophilum için ankA gen bölgesi yönünden üç izolatın kendi aralarında %99,8±0,2 identik olduğu, dünyadaki izolatlarla ise %0,9±0,3 farklılık gösterdiği; H. canis için 18S rRNA geni yönünden iki izolatın kendi aralarında %100 identik olduğu, dünyadaki izolatlarla ise %0,2±0,1 farklılık gösterdikleri tespit edilmiştir. Sonuç olarak bu çalışmayla Kayseri yöresinde köpeklerde kene kaynaklı protozoon ve rickettsial enfeksiyonların moleküler prevalansları saptanmış ve enfeksiyonlara yol açan türlerin çeşitli gen bölgeleri analiz edilerek moleküler karakterizasyonları yapılmıştır. Tüm izolatların Genbank kayıtları gerçekleştirilmiştir.Anahtar sözcükler: Anaplasmosis, babesiosis, ehrlichiosis, hepatozoonosis, köpek, moleküler karakterizasyon, Real Time PCR. B. gibsoni, A. phagocytophilum, H. canis, and B. canis vogeli was detected as 14.5%, 12.0%, 9.0%, 7.8%, 5.3%, and 2.3%, respectively while B.canis rossi was not detected in the examined samples. 182 (89.7%) out of the 400 samples were found to be infected with a single parasite species, and 21 (10.3%) were found to be infected with two species. According to the pairwise comparisons of 18S rRNA gene region of the isolates under B. canis canis, B.canis vogeli and B. gibsoni, 1.4±0.2%, 0.3±0.2%, and 0.9±0.3% genetic distance were detected with the other similar isolates from the world, respectively. Based on the 16S rRNA gene sequence alignments, 100% identity was found among the 3 isolates of E. canis while 0.1% genetic difference was determined with the isolates from the world. With respect to ankA gene region of A. phagocytophilum, 99.8±0.2% identity and 0.9±0.3% genetic difference were found among the 3 isolates obtained from Kayseri region and the isolates from the world, respectively. The 2 H. canis isolates were showed 100% identity to each other and 0.2±0.1% genetic difference were determined with the other isolates from the world with respect to 18S rRNA gene region. In conclusion, the molecular prevalence of tick-borne protozoon and rickettsial infections in dogs in Kayseri region was determined and the molecular characterizations of the obtained isolates were performed by analyzing the various gene regions in this study. All isolates were recorded to GenBank.

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Presence of Ehrlichia sp and Hepatozoon sp in dogs infested with ticks or with previous exposure attended at a veterinary centre in Trujillo, Peru

Alva Díaz,

Rojas-Moncada,

Vargas Rocha

et al. 2024

Rev. investig. vet. Perú

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The present study determined the prevalence of Ehrlichia sp morulae and Hepatozoon sp gamonts in dogs treated at a veterinary medical centre in the city of Trujillo. Blood samples were taken from dogs infested with ticks or exposed in the three months prior to the study. Blood samples (3 mL) were collected from the cephalic vein of 95 dogs in EDTA tubes. The samples were centrifuged. A drop of the phlogistic layer of each sample was placed on a slide, which was extended with a coverslip and subjected to Wright staining. The smears were observed under an optical microscope at 40X and 100X. A prevalence of Ehrlichia sp of 31.6 ± 9.4% was found. No presence of Hepatozoon sp gamonts was observed.

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Description of dogs naturally infected with Hepatozoon canis in the Aegean region of Turkey

Cited by 11 publications

References 25 publications

A Molecular survey of Hepatozoon canis in dogs in the Siirt province of Turkey

A Molecular survey of Hepatozoon canis in dogs in the Siirt province of Turkey

Köpeklerde kene kaynaklı bazı protozoon ve rickettsial enfeksiyonların Real Time PCR ile araştırılması ve saptanan izolatların moleküler karakterizasyonları

Presence of Ehrlichia sp and Hepatozoon sp in dogs infested with ticks or with previous exposure attended at a veterinary centre in Trujillo, Peru

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