Description of Corynebacterium poyangense sp. nov., isolated from the feces of the greater white-fronted geese (Anser albifrons)

Abstract

Two novel Gram-positive, non-spore-forming, facultatively anaerobic, non-motile, and short rods to coccoid strains were isolated from the feces of the greater white-fronted geese ( Anser albifrons ) at Poyang Lake. The 16S rRNA gene sequences of strains 4H37-19 T and 3HC-13 shared highest identity to that of Corynebacterium uropygiale Iso10 T (97.8%). Phylogenetic and phylogenomic analyses indicated that strains 4H37-19 T and 3HC-13 formed an independent clade within genus Corynebacterium and clustered with Corynebacterium uropygiale Iso10 T . The average nucleotide identity and digital DNA-DNA hybridization value between strains 4H37-19 and 3HC-13 and members within genus Corynebacterium were all below 95% and 70%, respectively. The genomic G + C content of strains 4H37-19 T and 3HC-13 was 52.5%. Diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidylcholine, and phosphatidyl inositol mannosides (PIM) were the major polar lipids, with C 18:1 ω 9 c , C 16:0 , and C 18:0 as the major fatty acids, and MK-8 (H 4 ), MK-8(H 2 ), and MK-9(H 2 ) as the predominant respiratory quinones. The major whole cell sugar was arabinose, and the cell wall included mycolic acids. The cell wall peptidoglycan contained meso -diaminopimelic acid ( meso -DAP). The polyphasic taxonomic data shows that these two strains represent a novel species of the genus Corynebacterium , for which the name Corynebacterium poyangense sp. nov. is proposed. The type strain of Corynebacterium poyangense is 4H37-19 T (=GDMCC 1.1738 T = KACC 21671 T ). Supplemental material for this article may be found at 10.1007/s12275-022-2089-9.

Isolation of Corynebacterium spp. and Arcanobacterium spp. from a chicken with keratoconjunctivitis

“…gibi) opportunistik patojenler; memeli ve kanatlı hayvanlarda deri, eklem ve iç organlar gibi çeşitli organlarda suppuratif enfeksiyonlara da sebep olmaktadır (13). Yapılan çalışmalarda, Korineform grubu olarak tanımlanan Corynebacterium ve Arcanobacterium cinsi bakterilerin kanatlılarda pnömoni, endokarditis, septik artiritis (13), infeksiyöz konjunktivitis (14,15) ve miks enfeksiyonlara neden olduğu (16); penguen (14,17), leylek (18), kartal (19) ötücü kuşlar (20), ağaçkakan (21), kaz (22), papağan (7), turna (10), kanarya (16), pelikan (23) ve tavuk (12) gibi pek çok yabani ve evcil kanatlı hayvan türünden izole edildiği bildirilmektedir. Çalışmamızda, Korineform bakterilerin göz, akciğer ve kalpten saf olarak izole edilmesi araştırıcıların a b a b bulgularını desteklemektedir.…”