Description of Bartonella ancashensis sp. nov., isolated from the blood of two patients with verruga peruana

Abstract

Three novel isolates of the genus Bartonella were recovered from the blood of two patients enrolled in a clinical trial for the treatment of chronic stage Bartonella bacilliformis infection (verruga peruana) in Caraz, Ancash, Peru. The isolates were initially characterized by sequencing a fragment of the gltA gene, and found to be disparate from B. bacilliformis. The isolates were further characterized using phenotypic and genotypic methods, and found to be genetically identical to each other for the genes assessed, but distinct from any known species of the genus Bartonella, including the closest relative B. bacilliformis. Other characteristics of the isolates, including their morphology, microscopic and biochemical properties, and growth patterns, were consistent with members of the genus Bartonella. Based on these results, we conclude that these three isolates are members of a novel species of the genus Bartonella for which we propose the name Bartonella ancashensis sp. nov. (type strain 20.00T = ATCC BAA-2694T = DSM 29364T).

Whole-Genome Analysis ofBartonellaancashensis, a Novel Pathogen Causing Verruga Peruana, Rural Ancash Region, Peru

“…20 and 41) were infected with B. ancashensis ( 17 – 19 ). DNA extracts from the 4 original whole blood specimens from these patients (20.00, 20.60, 41.00, and 41.60) were tested by using quantitative bacterial 16S rDNA PCR, standard bacterial 16S PCR, and B. ancashensis –specific PCR (Table 1).…”

“…Blood specimens were cultured for > 8 weeks as described ( 17 – 19 ). Bartonella culture-positive specimens were confirmed to be Bartontella species by using microbiological observations and molecular assays.…”

“…We previously reported identification of non- bacilliformis Bartonella in Peru during a clinical treatment trial ( 17 – 19 ). Using single-locus sequence typing, we identified 3 isolates (designated 20.00, 20.60, and 41.60) from 4 whole blood specimens collected from 2 patients (nos.…”

“…Using single-locus sequence typing, we identified 3 isolates (designated 20.00, 20.60, and 41.60) from 4 whole blood specimens collected from 2 patients (nos. 20 and 41) at day 0 or 60 during the clinical treatment trial in the Ancash region of Peru and found that these isolates had citrate synthase ( gltA ) gene sequences that diverged from that of B. bacilliformis reference strain KC583 ( 17 – 19 ). Multilocus sequencing typing and microbiological analyses indicated these 3 isolates are members of a novel Bartonella species, subsequently name B. ancashensis ( 17 ).…”

Development of fluorogenic probe-based and high-resolution melting-based polymerase chain reaction assays for the detection and differentiation of Bartonella quintana and Bartonella henselae

“…They are fastidious bacteria that cannot be cultured and have been increasingly reported in the literature (Deng et al, 2012). Thus far, 23 different Bartonella species have been described, and of these, 14 species have been proved to be or are considered likely to be human pathogens (Deng et al, 2012;Lin et al, 2010;Mullins et al, 2015). Most reported cases of Bartonella endocarditis are attributed to Bartonella henselae and Bartonella quintana, which can also cause chronic bacteremia, osteomyelitis, trench fever, bacillary angiomatosis, cat scratch disease, and peliosis hepatis (Edouard et al, 2015;Klein et al, 2002;Robbins et al, 2015;Mazur-Melewska et al, 2015a;Li et al, 2015;Mazur-Melewska et al, 2015b).…”