Description of a new relict eriophyoid mite, Loboquintus subsquamatus n. gen. &amp; n. sp. (Eriophyoidea, Phytoptidae, Pentasetacini) based on confocal microscopy, SEM, COI barcoding and novel CLSM anatomy of internal genitalia

Chetverikov, Philipp E.; Cvrković, Tatjana; Vidović, Biljana; Petanović, Radmila

doi:10.1007/s10493-013-9685-7

Cited by 29 publications

(23 citation statements)

References 16 publications

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“…In our previous papers we presented CLSM data on internal genitalia of four major phytoptid groups: pentasetacines (Chetverikov et al 2013, phytoptines Chetverikov 2014a, b), novophytoptines (Chetverikov in press) and mackiellines . In this study we investigated genital anatomy of nalepellines (gg.…”

Section: Clsm Study Of Internal Genitaliamentioning

confidence: 99%

“…A five-setous prodorsal shield is considered to be ancestral. Among contemporary eriophyoids, the most complete chaetom is possessed by only two monobasic genera-Pentasetacus (Schliesske 1985) and Loboquintus (Chetverikov et al 2013)-both of which are considered early-derivative mite genera and inhabit conifers. Pentasetacus araucariae lives on Araucaria araucana, whereas Loboquintus subsquamatus lives on Cupressus sempervirens.…”

Section: Introductionmentioning

confidence: 99%

“…The anatomy of internal genitalia was investigated in detail, under TEM, by Nuzzaci and Alberti (1996) and papers cited therein, and later reviewed and Exp Appl Acarol partially revised by Chetverikov (2012Chetverikov ( , 2014a and Chetverikov et al (2012Chetverikov et al ( , 2013Chetverikov et al ( , 2014aChetverikov et al ( , b, 2015 using confocal laser scanning microscopy (CLSM). In general, internal genitalia of eriophyoids include (1) a genital chamber and genital channel formed by two plates of longitudinal bridge, (2) a spermathecal apparatus comprising paired spermathecae and spermathecal tubes, and (3) a complex of associated apodemes (Fig.…”

Section: Introductionmentioning

confidence: 99%

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Basal divergence of Eriophyoidea (Acariformes, Eupodina) inferred from combined partial COI and 28S gene sequences and CLSM genital anatomy

et al. 2015

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Eriophyoids are an ancient group of highly miniaturized, morphologically simplified and diverse phytoparasitic mites. Their possible numerous host-switch events have been accompanied by considerable homoplastic evolution. Although several morphological cladistic and molecular phylogenetic studies attempted to reconstruct phylogeny of Eriophyoidea, the major lineages of eriophyoids, as well as the evolutionary relationships between them, are still poorly understood. New phylogenetically informative data have been provided by the recent discovery of the early derivative pentasetacine genus Loboquintus, and observations on the eriophyoid reproductive anatomy. Herein, we use COI and D1-2 rRNA data of 73 eriophyoid species (including early derivative pentasetacines) from Europe, the Americas and South Africa to reconstruct part of the phylogeny of the superfamily, and infer on the basal divergence of eriophyoid taxa. In addition, a comparative CLSM study of the female internal genitalia was undertaken in order to find putative apomorphies, which can be used to improve the taxonomy of Eriophyoidea. The following molecular clades, marked by differences in genital anatomy and prodorsal shield setation, were found in our analyses: Loboquintus(Pentasetacus((Eriophyidae + Diptilomiopidae)(Phytoptidae-1, Phytoptidae-2))). The results of this study suggest that the superfamily Eriophyoidea comprises basal paraphyletic pentasetacines (Loboquintus and Pentasetacus), and two large monophyletic groups: Eriophyidae s.l. [containing paraphyletic Eriophyidae sensu Amrine et al. 2003 (=Eriophyidae s.str.) and Diptilomiopidae sensu Amrine et al. 2003] and Phytoptidae s.l. [containing monophyletic Phytoptidae sensu Boczek et al. 1989 (=Phytoptidae s.str.) and Nalepellidae sensu Boczek et al. 1989]. Putative morphological apomorphies (including genital and gnathosomal characters) supporting the clades revealed in molecular analyses are briefly discussed.

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Section: Clsm Study Of Internal Genitaliamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Basal divergence of Eriophyoidea (Acariformes, Eupodina) inferred from combined partial COI and 28S gene sequences and CLSM genital anatomy

et al. 2015

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“…However, whereas there is complete suppression of eugenital setae in female eriophyoids (Loboquintus Chetverikov & Petanovic. may be an exception (Chetverikov et al 2013)), there is noticeable variation in this character in Tydeoidea. For example, there is complete suppression in both males and females in the subfamily Pronematinae, and females have fewer pairs of eugenital setae in the Triophtydeinae, but they are not completely suppressed.…”

Section: Legs (Ch 67 To 110) Presence/absence Of Lateral Claws (Ch mentioning

confidence: 97%

Morphological support for a clade comprising two vermiform mite lineages: Eriophyoidea (Acariformes) and Nematalycidae (Acariformes)

Bolton

Chetverikov

Klompen

2017

saa

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A morphology-based parsimony analysis (50 taxa; 110 characters) focused on relationships among basal acariform mites places Eriophyoidea (formerly in Trombidiformes) within Nematalycidae (Sarcoptiformes). Although both taxa have worm-like bodies, this grouping is unexpected because it combines obligate plant inhabitants (Eriophyoidea) with obligate inhabitants of deep-soil or mineral regolith (Nematalycidae sensu stricto). The Eriophyoidea + Nematalycidae clade, which is strongly supported (Bremer =5; bootstrap =85%), retains moderately good support (Bremer=3; bootstrap=66%) when three ratio-based characters pertaining to body shape are excluded. A total of eleven unambiguous synapomorphies unite all or some of Nematalycidae with Eriophyoidea. These include an annulated opisthosoma, an unpaired vi seta on the prodorsum, fusion of the palp trochanter with the palp femur, and a large relative distance between the anus and the genitalia. Three of the four Triassic genera of eriophyoid-like mites were also included in our analysis. Although all four genera have been tentatively placed within a new superfamily, we found no support for the monophyly of this group. One other interesting result of the analysis is the placement of a "living fossil", Proterorhagia oztotloica (Proterorhagiidae), as sister to the rest of Acariformes. However, support for this relationship is weak.

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“…The musculature of about 100 specimens, comprising five different species (Cecidophyopsis ribis, Loboquintus subsquamatus, Phytoptus avellanae, Trisetacus cf bagdasariani and T. cf grosmanni) were investigated in order to check if their reproductive anatomy had been interpreted correctly; the male genitalia of all five species had already been studied or briefly discussed Chetverikov et al 2013;2014a, b, c). For filamentous actin (F-actin) staining the mites were fixed in 4 % paraformaldehyde (PFA) in 0.1 M phosphate buffer, and stained in diluted TRITC-phalloidin (P1951 Sigma-Aldrich).…”

Section: Immunocytochemistrymentioning

confidence: 99%

Confocal microscopy reveals uniform male reproductive anatomy in eriophyoid mites (Acariformes, Eriophyoidea) including spermatophore pump and paired vasa deferentia

Chetverikov

2015

Exp Appl Acarol

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Male internal genitalia of eriophyoid mites comprise cuticle lined (anterior genital apodeme, genital chamber and ductus ejculatorius) and soft (paired vasa deferentia and single testis) organs. Three-dimensional reconstructions based on autofluorescence show that a thin-walled genital chamber is usually situated in a transverse plane and precisely copies the shape of the spermatophore. A thin vertical longitudinal plate (homologous to female longitudinal bridge) joins the genital chamber and ventral genital cuticle. The anterior genital apodeme is a separate vertical plate situated ahead of the genital chamber and provides a rigid support for it. The brightly autofluorescent ductus ejaculatorius starts from the posterior extremity of the genital chamber and goes backward. Proximally, the ductus ejaculatorius is tube-like, whereas distally, it is expanded into a sac. Confocal laser scanning microscopy observations on males, stained with phalloidin, indicate that the proximal ductus ejaculatorius is devoid of muscles whereas the distal ductus ejaculatorius possesses well-developed musculature (the "spermatophore pump"), appearing in 3D reconstructions as a hollow sphere with three apertures: one anterior and two posterior. Two thin-walled sausage-like vasa deferentia join the distal ductus ejaculatorius with a large single testis, each junction is encircled by a strong, ring-shaped muscle (musculus sphincter testiculodeferentis). Thin muscular fibers of the wall of the testis form a net-like pattern consisting of distinct polygonal cells. The topography of the male internal genitalia and musculature suggests that, contrary to previous observations, the spermatophore head might be extruded first and then the spermatophore stalk appears. The possible role of visceral and skeletal musculature, in the process of the expulsion of a spermatophore, is discussed.

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Description of a new relict eriophyoid mite, Loboquintus subsquamatus n. gen. & n. sp. (Eriophyoidea, Phytoptidae, Pentasetacini) based on confocal microscopy, SEM, COI barcoding and novel CLSM anatomy of internal genitalia

Cited by 29 publications

References 16 publications

Basal divergence of Eriophyoidea (Acariformes, Eupodina) inferred from combined partial COI and 28S gene sequences and CLSM genital anatomy

Basal divergence of Eriophyoidea (Acariformes, Eupodina) inferred from combined partial COI and 28S gene sequences and CLSM genital anatomy

Morphological support for a clade comprising two vermiform mite lineages: Eriophyoidea (Acariformes) and Nematalycidae (Acariformes)

Confocal microscopy reveals uniform male reproductive anatomy in eriophyoid mites (Acariformes, Eriophyoidea) including spermatophore pump and paired vasa deferentia

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