Description of a new polymer-secreting bacterium from a deep-sea hydrothermal vent, Alteromonas macleodii subsp. fijiensis, and preliminary characterization of the polymer

Raguénès, Gérard; Pignet, Patricia; Gauthier, Gregory M.; Peres, Alessandra; Christen, Richard; Rougeaux, Hélène; Barbier, Georges; Guézennec, Jean

doi:10.1128/aem.62.1.67-73.1996

Cited by 139 publications

(55 citation statements)

References 31 publications

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“…FT-IR spectroscopy was used to determine the presence of specific functional groups within the EPS (Raguénès et al, 1996(Raguénès et al, , 2004Braissant et al, 2007) such as carboxyl, sulfate, sulfinic acids, thiols, hydroxyl, and amino groups of purified EPS (Smith, 1996). EPS was purified by precipitation in ethanol and dialysis as described above.…”

Section: Ft-ir Spectroscopy Of Epsmentioning

confidence: 99%

Characteristics and turnover of exopolymeric substances in a hypersaline microbial mat

Braissant

Decho

Przekop

et al. 2009

FEMS Microbiology Ecology

142

149

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The properties and microbial turnover of exopolymeric substances (EPS) were measured in a hypersaline nonlithifying microbial mat (Eleuthera, Bahamas) to investigate their potential role in calcium carbonate (CaCO(3)) precipitation. Depth profiles of EPS abundance and enzyme activities indicated that c. 80% of the EPS were turned over in the upper 15-20 mm. Oxic and anoxic mat homogenates amended with low-molecular-weight (LMW) organic carbon, sugar monomers, and different types of EPS revealed rapid consumption of all substrates. When comparing the consumption of EPS with that of other substrates, only marginally longer lag times and lower rates were observed. EPS (5-8%) were readily consumed during the conversion of labile to refractory EPS. This coincided with a decrease in glucosidase activity and a decrease in the number of acidic functional groups on the EPS. Approximately half of the calcium bound to the EPS remained after 10 dialyses steps. This tightly bound calcium was readily available to precipitate as CaCO(3). We present a conceptual model in which LMW organic carbon complexed with the tightly bound calcium is released upon enzyme activity. This increases alkalinity and creates binding sites for carbonate and allows CaCO(3) to precipitate. Therefore, this model explains interactions between EPS and CaCO(3) precipitation, and underscores the critical role of aerobic and anaerobic microorganisms in early diagenesis and lithification processes.

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Section: Ft-ir Spectroscopy Of Epsmentioning

confidence: 99%

Characteristics and turnover of exopolymeric substances in a hypersaline microbial mat

Braissant

Decho

Przekop

et al. 2009

FEMS Microbiology Ecology

142

149

View full text Add to dashboard Cite

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“…For dominant patterns (more than 20 clones per pattern), ¢ve clones were partially sequenced to check for the homogeneity of the group. Sequencing procedures were as described previously [29] and the work was carried out by Genome Express S.A. (Grenoble, France) with automatic DNA sequencers (Perkin Elmer). Sequences were assembled with the SeqMan program (DNASTAR software; Madison, WI, USA).…”

Section: S Rdna Sequencing and Phylogenetic Analysismentioning

confidence: 99%

“…3^4 and Table 1). Several Firmicutes, N-and Q-Proteobacteria have been isolated from hydrothermal chimney samples [29,49,50] and from the micro£ora associated with A. pompejana [51,52]. One phylotype of group VII of Q-Proteobacteria was a⁄liated (91% sequence similarity) with the endosymbiont [18] of the Northeast Paci¢c vent vestimentifer Ridgeia piscesae inhabiting the same chimneys as P. palmiformis.…”

Section: Phylogenetic Analysis Of the Bacterial Assemblagesmentioning

confidence: 99%

Phylogenetic characterization of the bacterial assemblage associated with mucous secretions of the hydrothermal vent polychaete Paralvinella palmiformis

Alain

2002

FEMS Microbiology Ecology

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As part of an ongoing examination of microbial diversity associated with hydrothermal vent polychaetes of the family Alvinellidae, we undertook a culture-independent molecular analysis of the bacterial assemblage associated with mucous secretions of the Northeastern Pacific vent polychaete Paralvinella palmiformis. Using a molecular 16S rDNA-based phylogenetic approach, clone libraries were constructed from two samples collected from active sulfide edifices in two hydrothermal vent fields. In both cases, clone libraries were largely dominated by O-Proteobacteria. Phylotypes belonging to the Cytophaga^Flavobacteria and to the Verrucomicrobia were also largely represented within the libraries. The remaining sequences were related to the taxonomic groups Fusobacteria, Green non-sulfur bacteria, Firmicutes, Q-and N-Proteobacteria. To our knowledge, this is the first report of the presence of Verrucomicrobia, Fusobacteria and green non-sulfur bacteria on hydrothermal edifices. The potential functions of the detected bacteria are discussed in terms of productivity, recycling of organic matter and detoxification within the P. palmiformis microhabitat.

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“…In recent times, marine biofilms have also attracted attention because of their biotechnological potential. Vincent et al (1994) and Raguenes et al (1996) assessed the potential of biofilm-derived bacteria from deep-sea hydrothermal vents as producers of new exopolysaccharides: some showing a potential application in the food sector, while others exhibiting a bioactive behaviour. Bioactive polysaccharides are capable of eliciting physiological activities from the molecular to the organismal level (Hon 1996).…”

Section: Introductionmentioning

confidence: 99%

Characterization of extracellular polymers synthesized by tropical intertidal biofilm bacteria

et al. 2007

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Aim: This study was performed to determine the potential of tropical intertidal biofilm bacteria as a source of novel exopolymers (EPS). Methods and Results: A screening procedure was implemented to detect EPS‐producing biofilm bacteria. Isolates MC3B‐10 and MC6B‐22, identified respectively as a Microbacterium species and Bacillus species by 16S rDNA and cellular fatty acids analyses, produced different EPS, as evidenced by colorimetric and gas chromatographic analyses. The polymer produced by isolate MC3B‐10 displays significant surfactant activity, and may chelate calcium as evidenced by spectroscopic analysis. Conclusions: Polymer MC3B‐10 appears to be a glycoprotein, while EPS MC6B‐22 seems to be a true polysaccharide dominated by neutral sugars but with significant concentrations of uronic acids and hexosamines. EPS MC3B‐10 possesses a higher surfactant activity than that of commercial surfactants, and given its anionic nature, may chelate cations thus proving useful in bioremediation. The chemical composition of polymer MC6B‐22 suggests its potential biomedical application in tissue regeneration. Significance and Impact of the Study: This is the first report of a Microbacterium species producing EPS with surfactant properties, which expands our knowledge of the micro‐organisms capable of producing these biomolecules. Furthermore, this work shows that tropical intertidal environments are a nonpreviously recognized habitat for bioprospecting EPS‐producing bacteria, and that these molecules might be involved in ecological roles protecting the cells against dessication.

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Description of a new polymer-secreting bacterium from a deep-sea hydrothermal vent, Alteromonas macleodii subsp. fijiensis, and preliminary characterization of the polymer

Cited by 139 publications

References 31 publications

Characteristics and turnover of exopolymeric substances in a hypersaline microbial mat

Characteristics and turnover of exopolymeric substances in a hypersaline microbial mat

Phylogenetic characterization of the bacterial assemblage associated with mucous secretions of the hydrothermal vent polychaete Paralvinella palmiformis

Characterization of extracellular polymers synthesized by tropical intertidal biofilm bacteria

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