Description and phylogeny of the mitochondrial genome of Sabethes chloropterus, Sabethes glaucodaemon and Sabethes belisarioi (Diptera: Culicidae)

Aragão, Andressa de Oliveira; Neto, Joaquim Pinto Nunes; Cruz, Ana Cecília Ribeiro; Casseb, Samir Mansour Moraes; Cardoso, Jedson Ferreira; Silva, Sandro Patroca da; Ishikawa, Edna Aoba Yassui

doi:10.1016/j.ygeno.2018.03.016

Cited by 25 publications

(13 citation statements)

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“…They also comprised 37 functional and conserved subunits-13 PCGs, 22 tRNAs, and 02 rRNAs-and demonstrated similar patterns of gene positioning along the J (Foward) and N (Reverse) strands ( Fig. 1), similarly to that observed in studies of other species of Culicidae 32,[37][38][39][40][41][42][43][44][45] , and other groups of insects 31,[46][47][48] with reported mitogenomes.…”

Section: Resultssupporting

confidence: 73%

“…Anophelinae, containing seven taxa, demonstrated www.nature.com/scientificreports/ a monophyletic conformation previously predicted in morphological and molecular studies, focusing on the mitogenomes of species of the Anopheles genus 51,77-79 . Likewise, it was observed in Culicinae a monophyletic arrangement formed by 19 taxa distributed in three subclades, which corroborated with studies of morphological and molecular taxonomy, also based on the sequencing of the mitogenome of species belonging to the tribes Sabethini 42,43 , Culicini 39,40,45 , and, in particular, Aedini 2,37,80 , which in the phylogeny reconstructed, included three closely related groupings, corresponding to the Haemagogus, Aedes, and Psorophora genera. Currently, the Haemagogus genus, focus of this study, comprises 28 species, distributed in two subgenera: Conopostegus, with 04 species, and Haemagogus, with 24 species.…”

Section: Phylogenetic Analysis the Nucleotide Sequences Alignment Cosupporting

confidence: 83%

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Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae)

Silva

Cruz

Medeiros

et al. 2020

Sci Rep

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The genus Haemagogus (Diptera: Culicidae) comprises species of great epidemiological relevance, involved in transmission cycles of the Yellow fever virus and other arboviruses in South America. So far, only Haemagogus janthinomys has complete mitochondrial sequences available. Given the unavailability of information related to aspects of the evolutionary biology and molecular taxonomy of this genus, we report here, the first sequencing of the mitogenomes of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis. The mitogenomes showed an average length of 15,038 bp, average AT content of 79.3%, positive AT-skews, negative GC-skews, and comprised 37 functional subunits (13 PCGs, 22 tRNA, and 02 rRNA). The PCGs showed ATN as start codon, TAA as stop codon, and signs of purifying selection. The tRNAs had the typical leaf clover structure, except tRNASer1. Phylogenetic analyzes of Bayesian inference and Maximum Likelihood, based on concatenated sequences from all 13 PCGs, produced identical topologies and strongly supported the monophyletic relationship between the Haemagogus and Conopostegus subgenera, and corroborated with the known taxonomic classification of the evaluated taxa, based on external morphological aspects. The information produced on the mitogenomes of the Haemagogus species evaluated here may be useful in carrying out future taxonomic and evolutionary studies of the genus.

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Section: Resultssupporting

confidence: 73%

Section: Phylogenetic Analysis the Nucleotide Sequences Alignment Cosupporting

confidence: 83%

Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae)

Silva

Cruz

Medeiros

et al. 2020

Sci Rep

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“…gambiae was sequenced using the Sanger method on PCR amplified fragments 27 and further mitochondrial genomes were slowly sequenced along with whole genome projects. Currently, most studies have been using whole genome sequencing or PCR amplification followed by high throughput sequencing to characterize several mitogenomes at once 22 , 23 , 26 in a wide range of insect species showing promising results to reconstruct mitogenomes 32 , 43 . Here, we performed low-coverage whole genome sequencing to assemble and characterize the mitogenomes from seventeen mosquito species.…”

Section: Discussionmentioning

confidence: 99%

“…aranoides ), and the sister positioning of Trichoprosopon genus ( Tr. digitatum ) 22 , 26 , 54 (Fig. 4 ).…”

Section: Discussionmentioning

confidence: 99%

Culicidae evolutionary history focusing on the Culicinae subfamily based on mitochondrial phylogenomics

Silva

Machado

Paula

et al. 2020

Sci Rep

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Mosquitoes are insects of medical importance due their role as vectors of different pathogens to humans. There is a lack of information about the evolutionary history and phylogenetic positioning of the majority of mosquito species. Here we characterized the mitogenomes of mosquito species through low-coverage whole genome sequencing and data mining. A total of 37 draft mitogenomes of different species were assembled from which 16 are newly-sequenced species. We datamined additional 49 mosquito mitogenomes, and together with our 37 mitogenomes, we reconstructed the evolutionary history of 86 species including representatives from 15 genera and 7 tribes. Our results showed that most of the species clustered in clades with other members of their own genus with exception of Aedes genus which was paraphyletic. We confirmed the monophyletic status of the Mansoniini tribe including both Coquillettidia and Mansonia genus. The Aedeomyiini and Uranotaeniini were consistently recovered as basal to other tribes in the subfamily Culicinae, although the exact relationships among these tribes differed between analyses. These results demonstrate that low-coverage sequencing is effective to recover mitogenomes, establish phylogenetic knowledge and hence generate basic fundamental information that will help in the understanding of the role of these species as pathogen vectors.

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“…Further molecular taxonomy studies are required due to limitations of classical taxonomic keys. The keys used may be outdated regarding morphological 1 3 characters of the species described; molecular identification tools are not confined by this limitation and have allowed for the expansive characterization of jungle mosquitoes via genes (39) or complete mitochondrial genomes to identify species and reconstruct phylogenies (40) .…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of mosquitoes (Diptera: Culicidae) from the tropical rainforest of Sierra Nevada de Santa Marta, Colombia

Muñoz-Gamba

Laiton‐Donato

Perdomo-Balaguera³

et al. 2020

Preprint

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BACKGROUND The Sierra Nevada de Santa Marta rainforest has diverse fauna due to its position in northern Colombia, a Caribbean region with predominantly tropical, dry, and rainforest ecosystems in which there is a high diversity of mosquito species that may act as arbovirus vectors.OBJECTIVES The present study reports the molecular characterization of select mosquito species in this rainforest.METHODS Manual capture methods were used to collect mosquitoes, and the specimens were identified via classical taxonomy. The COI marker was used for species confirmation, and phylogenetic analysis was performed, using the neighbor-joining method, with the Kimura-2-Parameters model. and Wyeomyia luteoventralis were identified and intra-species variation >2% for most species. MAIN CONCLUSIONSWe report the first records on the genetic variability of mosquitoes in this area and phylogenetic reconstructions allowed for identification at the species level, and the corroboration by means of classical taxonomy suggested complementarity of both methods, which may be employed when morphological or molecular data are poor or not available. The genetic and morphological characterization of jungle mosquito populations will help to understand their biology.3

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Description and phylogeny of the mitochondrial genome of Sabethes chloropterus, Sabethes glaucodaemon and Sabethes belisarioi (Diptera: Culicidae)

Cited by 25 publications

References 28 publications

Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae)

Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae)

Culicidae evolutionary history focusing on the Culicinae subfamily based on mitochondrial phylogenomics

Molecular characterization of mosquitoes (Diptera: Culicidae) from the tropical rainforest of Sierra Nevada de Santa Marta, Colombia

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