Description and genetic analysis of H-2 associated genes controlling early embryo development

Abstract

This was produced from a copy of a document sent to us for microfilming. While tl most advanced technological means to photograph and reproduce this documei have been used, the quality is heavily dependent upon the quality of the materia submitted. The following explanation of techniques is provided to help you understar markings or notations which may appear on this reproduction. 1.The sign or "target" for pages apparently lacking from the document photographed is "Missing Page(s)". If it was possible to obtain the missing page(s) or section, they are spliced into the film along with adjacent pages. This may have necessitated cutting through an image and duplicating adjacent pages to assure you of complete continuity. 1. Nomenclature of monoclonals 76 2. Screening methods 90 3. Production of ascites tumors 93 4. Purification of monoclonal antibodies 94 a. Protein A sepharose purification 94 b. Ammonium sulphate precipitation 94 5. Analysis of monoclonals 99 a. Control ascites 99 b. Isotype analyses of monoclonal antibodies 99 c. Titration of monoclonal antibodies 99 d. Specificity of monoclonal antibodies 100 C. Detection of H-2 Antigens on Preimplantation Embryos 112 1. Indirect immunofluorescence 2. DNA synthesis assay