Description and evaluation of a short‐term reproduction test with the fathead minnow (Pimephales promelas)

Abstract

Due to the time and expense associated with full life-cycle testing, most current toxicity tests with fish do not explicitly consider reproductive output as an endpoint but, rather, focus on early life-stage survival and development. However, some classes of chemicals could adversely impact reproduction at concentrations below those that affect development. Further, estimates of the effects of toxic compounds on reproductive output can be critical to the ecological risk assessment process. In this manuscript, we describe a short-term reproduction test with the fathead minnow (Pimephales promelas) and evaluate the test using two model reproductive toxicants, methoxychlor (an estrogenic compound) and methyltestosterone (an androgenic chemical). The test is initiated with reproductively mature animals and is comprised of a pre-exposure phase of 14 to 21 d, followed by a chemical exposure of up to 21 d. During and at completion of the test, several endpoints related to reproductive fitness and endocrine function are assessed. Both chemicals evaluated in our study caused a significant decrease in fecundity of the fish at nominal concentrations of 5.0 micrograms/L (methoxychlor) and 0.2 mg/L (methyltestosterone). Methoxychlor decreased plasma concentrations of one or more steroids (testosterone, 11-ketotestosterone, beta-estradiol) in both sexes and caused a significant induction of plasma vitellogenin in males, a response consistent with activation of the estrogen receptor by the pesticide (or its metabolites). Methyltestosterone decreased plasma concentrations of sex steroids and adversely affected gonadal status (as evaluated by relative weight and histopathology) in both sexes. The androgenic nature of methyltestosterone was clearly expressed as masculinization of exposed females via formation of nuptial tubercles, structures normally present only in reproductively active males. The chemical also caused a significant induction of plasma vitellogenin in both males and females; this unexpected estrogenic response was most likely due to aromatization of the androgen to a form capable of binding to the estrogen receptor. These studies demonstrate the utility of this short-term assay for identifying chemicals that exert reproductive toxicity through alterations in endocrine systems controlled by estrogens and androgens.

The effects of silver nanoparticles on fathead minnow (Pimephales promelas) embryos

“…First, it is a well-characterized environmental model and the species is native to the US. Second, the availability of extensive genetic information on this fish lends to its efficient use in detailed molecular characterization of subcellular effects using available microarrays (Ankley et al 2001;Larkin et al 2007;Kane et al 2008) or other quantitative genetic molecular techniques. Finally, it is the preferred environmental model of the USEPA for use in toxicity studies and thus our knowledge of the biology of this animal will continue to increase through time.…”

The use of field-based mesocosm systems to assess the effects of uranium milling effluent on fathead minnow (Pimephales promelas) reproduction

“…They are also tolerant of a wide range of water quality parameters such as pH, temperature, alkalinity and hardness (Ankley and Villeneuve 2006). The partial lifecycle reproductive assay using fathead minnows as outlined by Ankley et al (2001) and modified by Rickwood et al (2006aRickwood et al ( , b, 2008) is a useful tool in assessing the effects on both adult fish and their offspring.…”

Environmental Risk Assessment of Pharmaceuticals

“…Synthetic estrogens such as ethinyl estradiol can feminize fish, reduce fertilization rates, and obliterate populations at concentrations from 1 to 6 ηg/L (Länge et al 2001, Parrott and Blunt 2005, Kidd et al 2007. Synthetic steroids used for growth promotion in cattle can masculinize fish (Ankley et al 2001(Ankley et al , 2003.…”