Describing the within laboratory and between laboratory agreement of a serum ELISA in a national laboratory network

Jordan, David; Kirkland, Peter D.; Morris, Stephen; Coilparampil, Ronald

doi:10.1016/j.prevetmed.2011.11.005

Cited by 6 publications

(5 citation statements)

References 4 publications

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“…The consistency of the PRRSV oral fluid ELISA was quantified by estimating reliability coefficients (ρ) for assay repeatability (within laboratory) and assay reproducibility (between laboratories) using the results from oral fluid samples of known (n = 39) and unknown (n = 224) PRRSV antibody status, respectively. 2,10 For calculation of repeatability reliability coefficients, the model and associated parameters were based on n samples with d repeated measurements of a continuous variable x and denoted by x ij , the jth measurement made on the ith subject (for i = 1, . .…”

Section: Discussionmentioning

confidence: 99%

“…That is, given the same assay, reliability coefficient estimates will be affected as the concentration and/or prevalence of the analyte in the population of samples tested varies. 2 Repeatability reliability coefficients were calculated using 13 samples tested on each ELISA plate in every laboratory (Table 3). Coefficients ranged from 0.724 to 0.997 (phase 1) and from 0.953 to 0.998 (phase 2).…”

Section: Discussionmentioning

confidence: 99%

“…That is, given the same assay, reliability coefficient estimates will be affected as the concentration and/or prevalence of the analyte in the population of samples tested varies. 2…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Ring test evaluation of the repeatability and reproducibility of a Porcine reproductive and respiratory syndrome virus oral fluid antibody enzyme-linked immunosorbent assay

et al. 2012

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Abstract. The precision of a Porcine reproductive and respiratory syndrome virus (PRRSV) oral fluid antibody enzymelinked immunosorbent assay (ELISA) was evaluated by calculating reliability coefficients for assay repeatability (within laboratory) and assay reproducibility (between laboratories). Randomly ordered oral fluid samples of known (n = 39) and unknown (n = 224) PRRSV antibody status were tested in 12 diagnostic laboratories. Each laboratory tested the samples twice, first using an antibody ELISA kit and reagents provided to them (phase 1) and then using an ELISA kit and reagents configured in their respective laboratory (phase 2). Repeatability (within laboratory) reliability coefficients calculated using results from samples of known PRRSV antibody status ranged from 0.724 to 0.997 in phase 1 and from 0.953 to 0.998 in phase 2. Reproducibility (between laboratories) reliability coefficients were calculated for 3 conditions: case 1-samples of unknown status (n = 224); case 2-samples of known status (n = 39), and case 3-all samples (n = 263). Among the 3 cases, reliability coefficients ranged from 0.937 to 0.964 in phase 1 and from 0.922 to 0.935 in phase 2. For case 3, it was estimated that 96.67% of the total variation in phase 1 and 93.21% in phase 2 could be attributed to the oral fluid samples themselves. Overall, the PRRSV oral fluid antibody ELISA was highly repeatable and reproducible. The current study supports the routine use of this test in laboratories providing diagnostic service to pig producers.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…That is, given the same assay, reliability coefficient estimates will be affected as the concentration and/or prevalence of the analyte in the population of samples tested varies. 2…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Ring test evaluation of the repeatability and reproducibility of a Porcine reproductive and respiratory syndrome virus oral fluid antibody enzyme-linked immunosorbent assay

et al. 2012

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“…Reliability was estimated by intraclass correlation coefficient (ICC, ρ) and using a latent class approach (equation [2]). 6,9,15 ρ σ…”

Section: Discussionmentioning

confidence: 99%

Assessment of a fluorescent antibody test for the detection of antibodies against epizootic bovine abortion

et al. 2014

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The current study was directed at developing and validating an indirect fluorescent antibody test (IFAT) capable of detecting antibodies specific for the agent of epizootic bovine abortion (aoEBA). Sensitivity and specificity was determined by comparing antibody titers from 114 fetuses infected with aoEBA with 68 fetuses diagnosed with alternate infectious etiologies. Data established specificity at 100% and sensitivity at 94.7% when cutoff criteria for a positive test were assigned at a titer of ≥1,000. Potential cross-reactivity was noted in samples from 3 fetuses with antibody titers of 10 or100; all were infected with Gram-positive organisms. The remaining 65 fetuses infected with microbes other than aoEBA, and an additional 12 negative reference sera, did not have detectable titers. The IFAT-based serology assay is rapid, reproducible, and unaffected by fluid color or opacity. Total fetal immunoglobulin (Ig)G was also evaluated as an aid for diagnosing EBA. Significantly higher concentrations of IgG were identified in fetuses infected with aoEBA as compared to those with alternate infectious etiologies. The presence of IgG is a sensitive indicator of EBA and increases the specificity of FAT-based serologic diagnosis when titers are 10 or 100. Taken together, serology and IgG analyses suggest that the incidence of EBA may be underestimated.

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Intra- and inter-laboratory agreement of the disc diffusion assay for assessing antimicrobial susceptibility of porcine Escherichia coli

Badger

Abraham

Stryhn

et al. 2019

Preventive Veterinary Medicine

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Describing the within laboratory and between laboratory agreement of a serum ELISA in a national laboratory network

Cited by 6 publications

References 4 publications

Ring test evaluation of the repeatability and reproducibility of a Porcine reproductive and respiratory syndrome virus oral fluid antibody enzyme-linked immunosorbent assay

Ring test evaluation of the repeatability and reproducibility of a Porcine reproductive and respiratory syndrome virus oral fluid antibody enzyme-linked immunosorbent assay

Assessment of a fluorescent antibody test for the detection of antibodies against epizootic bovine abortion

Intra- and inter-laboratory agreement of the disc diffusion assay for assessing antimicrobial susceptibility of porcine Escherichia coli

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