Desaturation of Fatty Acids Associated with Monogalactosyl Diacylglycerol: The Effects of San 6706 and San 9785

Lem, Nora W.; Williams, John P.

doi:10.1104/pp.68.4.944

Cited by 30 publications

(13 citation statements)

References 27 publications

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“…A further desaturation occurs after the transportation of PC to the plastid outer envelope by phospholipid transfer protein (23) where the DAG moiety is used to form MGDG (19), and sn-I 18:2 is subsequently desaturated to 18:3 (21). This final desaturation step has only been reported as significant in leaves (18,23) with very little 18:2 transferred to MGDG in seed homogenates (26). This interpretation of the pathways for polyunsaturated fatty acid biosynthesis is consistent with recent findings ( 15,29).…”

supporting

confidence: 80%

“…It is proposed that the IXLIN genotype contains genes which reduce the activity of the eukaryotic sn-2 PC desaturases in the endoplasmic reticulum. Since other modifications to 18:2 desaturase activity have interfered with the desaturation of 16:2 and 18:2 on MGDG (3,18,21), this system represents the first analysis of genes which modify the activity of the sn-2 linoleoyl phosphatidylcholine desaturase/ s of the endoplasmic reticulum in the eukaryotic pathway.…”

Section: Discussionmentioning

confidence: 99%

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A Study of Phospholipids and Galactolipids in Pollen of Two Lines of Brassica napus L. (Rapeseed) with Different Ratios of Linoleic to Linolenic Acid

Evans¹,

Sang²,

Cominos³

et al. 1990

Plant Physiol.

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The phospholipids and galactolipids of the pollen-coat and internal domains of two lines of Brassica napus, Wesroona and IXLIN, with different linoleic/linolenic acid ratios (18:2/18:3) have been characterized by normal phase silica high performance liquid chromatography and gas liquid chromatography. The polar lipids of the pollen-coat are similar to leaf lipids in the high proportion of galactolipids (almost 50%) and the fatty acids; 18:3, palmitic (16:0) and hexadecatrienoic (16:3). In contrast, the pollen internal domain, although rich in 18:3, 18:2 and 16:0, is composed primarily of phosphatidyl-choline, -ethanolamine, and -inositol whose 18:2/18:3 ratio is correlated with that of the seed generation. The difference between the two divergent 18:2/18:3 ratio lines is most evident in the internal domain phospholipids. The 18:2/18:3 ratio of the galactolipids of both pollen domains is not significantly effected by the line genotype. The results are interpreted in terms of the previously described 'prokaryotic' and 'eukaryotic' plant desaturation pathways (PG Roughan, CR Slack [1982] Annu Rev Plant Physiol 33: 97-132). We propose that the eukaryotic pathway is the major desaturation pathway providing polyunsaturated fatty acids to the haploid-specified internal domain in which the IXLIN genotype modifies the activity of the sn-2 linoleoyl phosphatidylcholine desaturase/s of the endoplasmic reticulum. In the diploid-specified pollen-coat, our evidence suggests that a combination of the prokaryotic and eukaryotic pathways contribute polyunsaturated fatty acids.

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supporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

A Study of Phospholipids and Galactolipids in Pollen of Two Lines of Brassica napus L. (Rapeseed) with Different Ratios of Linoleic to Linolenic Acid

Evans¹,

Sang²,

Cominos³

et al. 1990

Plant Physiol.

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“…The carotenoid biosynthesis inhibitors, fluridone and norfltirazon, have been reported to lead to decreases in photosynthesis (Lem and Williams 1981), ribosome number per plastid and plastid rRNA synthesis (Bartles andWatson 1978, ReiB et al, 1983), and chlorophyll per leaf (Vaisberg and Schiff 1976), Lipid composition is also affected (Lem and Williams 1981),…”

Section: Discussionmentioning

confidence: 99%

Development and accumulation of ABA in fluridone‐treated and drought‐stressed Vicia faba plants under different light conditions

Попова

Riddle

1996

Physiologia Plantarum

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The effects of fluridone on guard cell morphology, chloroplast ultrastructure and accumulation of drought stress‐induced abscisic acid (ABA) were studied in Vicia faba L. plants grown under different light conditions. Drought stress was induced by allowing the leaves to lose 12% of their fresh weight. The appearance of defective and undeveloped stomata, and chloroplasts with a destroyed thylakoid membrane system was found in fluridone‐treated plants grown at a photosynthetic photon flux (PPF) of 600 μmol m‐2 s‐1. Plants grown at a PPF of 40 μmol m‐2 s‐1 had diminished levels of ABA after imposition of dehydration. Fluridone treatment reduced the level of ABA in both unstressed and dehydrated leaves. Accumulation of ABA in the control plants was considerably reduced when they were exposed to dark periods of 24, 48 and 72 h just before imposition of the stress. Twenty‐four hours after the dark treatment dehydration of the leaves resulted in a 3‐fold decrease in the level of stress‐induced ABA, and 72 h after dark treatment the amount of stress‐induced ABA approximated the prestressed values. Fluridone‐treated plants failed to accumulate ABA under water stress. In addition to functionally active chloroplasts, well‐developed and functional stomata are required for drought stress to elicit a rise in ABA.

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“…The pyridazinone compound SAN 9785 [4-chloro-5-(dimethylamino)-2-phenyl-3(2H)-pyridazinone] has been shown to be a specific inhibitor of the desaturation reaction betwen linoleic and linolenic acids (St. John 1976;Lem and Williams 1981). This compound, therefore, seems to be a promising tool with which to study the role of linolenic acid within the photosynthetic membranes.…”

Section: Introductionmentioning

confidence: 95%

Photosynthetic characteristics of detached barley leaves during greening in the presence of SAN 9785

Laskay

Lehoczki

Dobi

et al. 1986

Planta

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The effects of the pyridazinone compound SAN 9785 on the photosynthetic competence of leaves, on the photochemical activity of isolated thylakoids and on the formation and spectral properties of chlorophyll-protein complexes were studied during a 72-h greening period of detached etiolated leaves of barley (Hordeum vulgare L. cv. Horpácsi kétsoros). It was established that i) the photosynthetic capacity of the leaves decreased considerably (by 80 and 90%, as determined by(14)CO2 fixation and fast fluorescence induction measurements, respectively); ii) the photochemical activity of isolated thylakoids from water to potassium ferricyanide and from dichlorophenol indophenol/ascorbate to methylviologen exhibited only slight reductions when expressed on a chlorophyll basis compared with the control; iii) the slow fluorescence induction curves of the treated leaves demonstrated the presence of a peculiar fluorescence component interrupting the quenching of fluorescence at around 1 min illumination; iv) a shortage of the chlorophyll-protein complex of photosystem I (CPI) occurred with a higher content of the monomer of the light harvesting complex in the thylakoids of treated leaves; and v) the fluorescence spectrum of the CPI band present in treated leaves indicates the destruction of the structural integrity of this complex during isolation from the membrane.

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Desaturation of Fatty Acids Associated with Monogalactosyl Diacylglycerol: The Effects of San 6706 and San 9785

Cited by 30 publications

References 27 publications

A Study of Phospholipids and Galactolipids in Pollen of Two Lines of Brassica napus L. (Rapeseed) with Different Ratios of Linoleic to Linolenic Acid

A Study of Phospholipids and Galactolipids in Pollen of Two Lines of Brassica napus L. (Rapeseed) with Different Ratios of Linoleic to Linolenic Acid

Development and accumulation of ABA in fluridone‐treated and drought‐stressed Vicia faba plants under different light conditions

Photosynthetic characteristics of detached barley leaves during greening in the presence of SAN 9785

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