Desalted and lyophilized bovine seminal plasma delays induction of the acrosome reaction in frozen-thawed bovine spermatozoa in response to calcium ionophore

Almadaly, Essam A.; Hoshino, Youichirou; Ueta, T.; Mukoujima, Koushi; Shukry, Mustafa; Farrag, Foad; El-Kon, Ismail; Kita, Kazuo; Murase, Tetsuma

doi:10.1016/j.theriogenology.2014.09.004

Cited by 15 publications

(16 citation statements)

References 58 publications

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“…Protein composition of the seminal plasma varies widely among species (Druart et al, ). Many of the identified seminal plasma proteins (SPPs) from ungulates are predicted to function in fertility (bulls [Souza, Moura, Monaco, & Killian, ], boars [Caballero et al, ], stallions [Töpfer‐Petersen, Ekhlasi‐Hundrieser, Kirchhoff, Leeb, & Sieme, ], and buffalos [Harshan et al, ]); cryoprotection (bulls [Almadaly et al, ] and rams [Pérez‐Pé, Cebriàn‐Pérez, & Muiño‐Blanco, ]); or sperm viability (bulls and rams [Barrios et al, ]). Thus, SPPs are thought to improve the function of semen extenders and, consequently, fertility.…”

Section: Introductionmentioning

confidence: 99%

SL15: A seminal plasma‐derived lectin from the sperm of llama (Lama glama)

Zampini

Sequeira

Argañaraz

et al. 2017

Molecular Reproduction Devel

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The oviductal sperm reservoir of South American camelids is formed when sperm bind to N-acetylgalactosamine (GalNAc) on the surface of oviductal epithelium. The aim of this study was to characterize the GalNAc-binding proteins on llama sperm, and to establish their origin. Sperm-adsorbed proteins were extracted with 0.5 M KCl in Hepes-balanced salts. Sperm-adsorbed and seminal plasma proteins were then subjected to ligand blotting for their GalNAc affinity, and the labeled bands were identified by mass spectrometry. Three proteins were identified in seminal plasma versus only one in the sperm-adsorbed population; SL15, a seminal lectin, was common to both. SL15 is a homologue of Zymogen granule protein 16, homolog B-like, which belongs to the Jacalin-related lectin family. This lectin is likely presented to sperm via seminal plasma since epididymal sperm are not capable of binding GalNAc, whereas ejaculated sperm does, and its transcript was enriched predominantly in the prostate and bulbourethral glands. This is the first report of a seminal lectin in South American camelids that originates in the male reproductive tract, and is probably involved in sperm reservoir formation.

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Section: Introductionmentioning

confidence: 99%

SL15: A seminal plasma‐derived lectin from the sperm of llama (Lama glama)

Zampini

Sequeira

Argañaraz

et al. 2017

Molecular Reproduction Devel

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“…The main point of interest is that protein bands of molecular masses 80, 27.5, 21, 19, 17.5, 9 and 8.5 kDa may be candidates of bovine SPPs that need further investigations. Both the current study and our recent study (Almadaly et al, 2015) are unique in detection of high (185.8 kDa) molecular weight protein which may be a novel SPP in bovine semen or it may correspond to fast myosin heavy chain extraocular according to Odhiambo & Dailey (2011). Also, the detected protein of 10 kDa may correspond to secretoglobin 1D according to Boe-Hansen et al (2015).…”

Section: Discussionmentioning

confidence: 52%

“…Since, knowledge of the biochemical composition of SP is essential to improve storage conditions and to predict both the storage tolerance and fertilising ability of spermatozoa. Moreover, recently Almadaly et al (2015) confirmed that desalted SP added to semen extender has beneficial effects on sperm cell function of frozenthawed Japanese Black bull spermatozoa. Therefore, the current study aimed to identify protein and lipid molecules in raw and desalted SP of Holstein-Friesian bulls of proven fertility in trial to detect some biochemical markers of bull fertility and to improve fertility of frozen semen.…”

Section: Introductionmentioning

confidence: 83%

“…The present study represents a continuing effort following primary work focused on the incorporation of desalted and lyophilised SP into cryoprotective diluent before cryopreservation to minimise the freezethaw damage of bull spermatozoa (Almadaly et al, 2015). Semen quality is considered a risk factor for subfertility and/or infertility in dairy and beef cattle farms.…”

Section: Discussionmentioning

confidence: 99%

“…Raw SP was desalted by using gravity protocol according to the manufacturer's instructions. Only 2.5 mL of raw SP obtained from each ejaculate was applied to Sephadex G-25 (GE Healthcare, UK) desalting columns with a molecular weight cut-off being 5 kDa to obtain eluates of high protein concentration and of low K + ion concentration (Almadaly et al, 2015). Likewise, the collected eluates (desalted SP) were examined for Na + , K + and Cl − ions concentration using the automated equipment.…”

Section: Recovery and Desalting Of Spmentioning

confidence: 99%

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Protein and lipid species in seminal plasma of fertile Holstein-Friesian bulls

Almadaly¹,

Farrag²,

El-Nasr³

2019

BJVM

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Protein and lipid molecules in seminal plasma (SP) collected from fertile bulls were investigated. Semen was collected from 10 bulls (2 ejaculates each) and examined for standard semen analysis. Raw SP was recovered by centrifugation and total protein (TP) concentration was determined using a refractometer. Raw SP was desalted using a Sephadex G-25 desalting column then both raw and desalted SP was subjected to SDS-PAGE. Neutral lipids and phospholipids of raw and desalted SP were separated by thin-layer chromatography (TLC). The results revealed that, all bulls had normal semen characteristics and TP concentration in SP ranged from 7.0 to 10.4 g/dL except bull No. 6 had a relatively low concentration of 4.9 to 6.8 g/dL. Neither proteins nor lipids species were different between raw and desalted SP. Seventeen proteins were detected ranging from 8.5 to 185.8 kDa, and those of 12, 13.5, 15, 21, 23 and 38 kDa were predominant. Notably, proteins of 10, 17.5, 19, 21, 80 and 185.8 kDa might be new candidates of SP proteins (SPPs). The detected neutral lipid spots corresponded to cholesterol, 1,2-dimyristoyl glycerol, 1,2-dioleoyloglycerol, 1,3-dimyristoyl glycerol and 1,3-dioleoyloglycerol. The detected phospholipids spots corresponded to non-migrating phospholipids, sphingomyelin (SM), phosphatidylcholine (PC), phosphatidylserine (PS), phosphatidylinositol (PI), phosphatidylethanolamine (PE), cerebroside and polyglycerol phosphatide. Cholesterol represents the major molecule of neutral lipids, whereas SM, PC, PI and PE represent the major phospholipids. Noteworthy, there were 2 species of diacylglycerol (DAG) and 3 species of PI in bovine SP. In conclusion, this study gave a general picture of SP protein and lipid species in fertile bull semen, which might serve as fundamental knowledge for either semen analysis or prediction of male fertility.

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Evolution and function of mammalian binder of sperm proteins

et al. 2015

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Binder of sperm (BSP) proteins are ubiquitous among mammals and have been extensively investigated over the last three decades. They were first characterized in bull seminal plasma and have now been identified in more than 15 different mammalian species where they represent a superfamily. In addition to sharing a common structure, BSP proteins share many characteristics. They are expressed by seminal vesicles and epididymides, interact with similar ligands and bind to the outer leaflet of sperm membranes via an interaction with choline phospholipids. In addition to playing a major role in sperm capacitation, they are implicated as molecular chaperones in sperm motility and viability, in the formation of the oviductal sperm reservoir, in the regulation of cell volume and possibly in the interaction between sperm and oocytes, making them crucial multifunctional proteins. Furthermore, BSP proteins can bind to egg yolk low-density lipoproteins and milk components, an interaction important for the protection of sperm during semen preservation in liquid or frozen state. Our current knowledge of BSP proteins strongly emphasizes their fundamental importance in male fertility and in the optimization of semen preservation techniques. Much work is still ahead in order to fully understand all the mysteries of BSP proteins.

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Desalted and lyophilized bovine seminal plasma delays induction of the acrosome reaction in frozen-thawed bovine spermatozoa in response to calcium ionophore

Cited by 15 publications

References 58 publications

SL15: A seminal plasma‐derived lectin from the sperm of llama (Lama glama)

SL15: A seminal plasma‐derived lectin from the sperm of llama (Lama glama)

Protein and lipid species in seminal plasma of fertile Holstein-Friesian bulls

Evolution and function of mammalian binder of sperm proteins

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