Des‐gamma‐carboxyprothrombin detection by immunoblotting after polyacrylamide gel affinoelectrophoresis in human plasmas

Abstract

In the absence of vitamin K or in the presence of the vitamin K antagonists, abnormal nonfunctional forms of prothrombin circulate in the blood. A reliable and reproducible technique, derived from traditional crossed affinoimmunoelectrophoresis in presence of calcium lactate, was developed and optimized. The technique is based on nondenaturing polyacrylamide gel affinoelectrophoresis, with calcium lactate, of plasma samples, followed by immunoblotting with rabbit anti-human prothrombin serum and detection with an anti-rabbit immunoglobulin peroxidase conjugate. Depending on the plasmas, one or two bands were visualized and quantified by densitometry of the immunoblots. The technique was able to detect abnormal des-gamma-carboxylated prothrombins at concentration of 0.1 microgram/mL.

Food sources and correlates of sodium and potassium intakes in Flemish pre-school children

“…However, this finding is in contrast to what has been described in other countries (28)(29)(30)(31) . It is noteworthy that the lack of information on discretionary salt could bias this conclusion as there might be differences in the use of discretionary salt between different socio-economic classes.…”

“…Our finding that some participant characteristics such as perceived family income and size were not associated with Na and K intakes and their ratio compared well with the results derived from French children (26) and suggests that food habits are relatively uniform across the social categories with respect to Na or K intake. However, this finding is in contrast to what has been described in other countries (28)(29)(30)(31) . It is noteworthy that the lack of information on discretionary salt could bias this conclusion as there might be differences in the use of discretionary salt between different socio-economic classes.…”

Vitamin K supplementation for cystic fibrosis

“…Vitamin K-specific laboratory outcomes plasma level of vitamin K1 (measured by high performance liquid chromatography (HPLC) and fluorescence detection (Wang 2004)proteins induced by vitamin K absence or antagonism factor II (PIVKA II) levels (measured by enzyme-linked immunosorbent assay (ELISA)) (Belle 1991; Belle 1995)…”

“…proteins induced by vitamin K absence or antagonism factor II (PIVKA II) levels (measured by enzyme-linked immunosorbent assay (ELISA)) (Belle 1991; Belle 1995)…”

Vitamin K supplementation for cystic fibrosis

“…Serum levels a. serum undercarboxylated osteocalcin (ucOC) b. ucOC/cOC (carboxylated osteocalcin) ratio (UCR) 4. Vitamin K-specific laboratory outcomes a. plasma level of vitamin K1 (measured by high performance liquid chromatography (HPLC) and fluorescence detection (Wang 2004) b. proteins induced by vitamin K absence or antagonism factor II (PIVKA II) levels (measured by enzyme-linked immunosorbent assay (ELISA))(Belle 1991;…”