Dermatopontin, a shell matrix protein gene from pearl oyster Pinctada martensii, participates in nacre formation

Jiao, Yu; Wang, Huan; Du, Xin; Zhao, Xiyang; Wang, Qingheng; Huang, Ronglian; Deng, Yuewen

doi:10.1016/j.bbrc.2012.07.099

Cited by 38 publications

(24 citation statements)

References 20 publications

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“…For in situ studies, measurements of aragonite formation were conducted within shells over defined periods of time when certain proteins are expressed or introduced into the shell environment [34,35,37,40,45]. In both sets of studies aragonite formation was confirmed in the presence of multiple nacre proteins [34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51]. In addition, some nacre proteins exhibited the ability to inhibit calcite growth [41,[46][47][48] or induce metastable vaterite formation as well [48,50,51].…”

Section: Protein-polymorph Formation and Stabilization-what Do We Curmentioning

confidence: 99%

“…The major reason for this is the lack of standardization: even though conditions for studying non-classical nucleation schemes were published nearly 8 years ago [30] there are few examples in the protein literature that utilize these methods of generating ion supersaturation and subsequent nucleation. Further, many studies relied on different ion concentration levels, control conditions, time periods, temperatures, pH values, and most importantly, protein quantities, in their assay experiments [34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53]. Typically, in most cases protein quantities have been reported as mass/volume values [34][35][36][37][38][39][40][41][42][43][44][45]52,53] rather than mole values [46][47][48][49][50][51], and this lack of standardization prevents cross-comparisons between protein studies.…”

Section: Protein-polymorph Formation and Stabilization-what Do We Curmentioning

confidence: 99%

“…For in vitro-based studies, several different carbonate-based assays have been used to achieve supersaturation conditions necessary for calcium carbonate crystal growth, with no control over pH, and the duration of these nucleation experiments were variable (i.e., from several hours up to 7 days) [36,38,39,[41][42][43][44][46][47][48][49][50][51]. Further, some studies utilized Mg(II) ions in the assay mixture to mimic seawater conditions and promote and stabilize aragonite [36,44].…”

Section: Protein-polymorph Formation and Stabilization-what Do We Curmentioning

confidence: 99%

“…For aragonite, what we must draw from are studies conducted with mollusk shell nacre-associated protein sequences [34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51]. For in vitro-based studies, several different carbonate-based assays have been used to achieve supersaturation conditions necessary for calcium carbonate crystal growth, with no control over pH, and the duration of these nucleation experiments were variable (i.e., from several hours up to 7 days) [36,38,39,[41][42][43][44][46][47][48][49][50][51].…”

Section: Protein-polymorph Formation and Stabilization-what Do We Curmentioning

confidence: 99%

“…Second, and perhaps most importantly, genomic and proteomic studies of biomineralization proteins have uncovered a vast repertory of proteins [6][7][8]13,14,[26][27][28] that could potentially manage many aspects of proposed nucleation processes, including polymorphism. However, although many protein studies have confirmed that polymorphs do form when proteins are present [34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53], these studies have not provided information that is needed for detailing the mechanisms of polymorph formation, or, have utilized a variety of testing methods that prevent cross-comparisons of datasets necessary for mechanism building [34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51]…”

Section: Introductionmentioning

confidence: 99%

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Polymorphs, Proteins, and Nucleation Theory: A Critical Analysis

Evans

2017

Minerals

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Over the last eight years new theories regarding nucleation, crystal growth, and polymorphism have emerged. Many of these theories were developed in response to observations in nature, where classical nucleation theory failed to account for amorphous mineral precursors, phases, and particle assembly processes that are responsible for the formation of invertebrate mineralized skeletal elements, such as the mollusk shell nacre layer (aragonite polymorph) and the sea urchin spicule (calcite polymorph). Here, we summarize these existing nucleation theories and place them within the context of what we know about biomineralization proteins, which are likely participants in the management of mineral precursor formation, stabilization, and assembly into polymorphs. With few exceptions, much of the protein literature confirms that polymorph-specific proteins, such as those from mollusk shell nacre aragonite, can promote polymorph formation. However, past studies fail to provide important mechanistic insights into this process, owing to variations in techniques, methodologies, and the lack of standardization in mineral assay experimentation. We propose that the way forward past this roadblock is for the protein community to adopt standardized nucleation assays and approaches that are compatible with current and emerging nucleation precursor studies. This will allow cross-comparisons, kinetic observations, and hopefully provide the information that will explain how proteins manage polymorph formation and stabilization.

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Section: Protein-polymorph Formation and Stabilization-what Do We Curmentioning

confidence: 99%

Section: Protein-polymorph Formation and Stabilization-what Do We Curmentioning

confidence: 99%

Section: Protein-polymorph Formation and Stabilization-what Do We Curmentioning

confidence: 99%

Section: Protein-polymorph Formation and Stabilization-what Do We Curmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Polymorphs, Proteins, and Nucleation Theory: A Critical Analysis

Evans

2017

Minerals

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A novel nacre matrix protein hic24 in Hyriopsis cumingii is essential for calcium carbonate nucleation and involved in pearl formation

Liu

Liu²,

Jin³

et al. 2018

Biotech and App Biochem

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Matrix proteins play important roles in molluscan shell biomineralization, which helps in the understanding of mechanisms associated with pearl formation. In this study, we characterized the gene encoding a novel shell-matrix protein, hic24, in Hyriopsis cumingii and investigated its structure and function. The full cDNA sequence of hic24 is 756 bp, with an open reading frame of 654 bp encoding 217 amino acids, including a signal peptide of 18 amino acids. Sequence analysis revealed that the protein is ∼23.5 kDa, and that Gly accounted for 11.5% of the total amino acid content. Secondary structure prediction indicated a structure comprised predominantly by β-folds. Quantitative real-time polymerase chain reaction and in situ hybridization indicated that hic24 is expressed in the dorsal epithelial cells of the mantle, indicating hic24 as a nacreous-layer matrix protein. Additionally, hic24 expression patterns during pearl biomineralization showed that hic24 regulates the growth of the later nacreous layer. After attenuating hic24 expression by RNA interference in the mantle, we observed that hic24 plays a role in biomineralization of the shell nacre by inhibiting calcium carbonate nucleation.

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Physical Phenomena Governing Mineral Morphogenesis in Molluscan Nacre

Gránásy,

Rátkai,

Zlotnikov

et al. 2023

Small

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Mollusks, as well as many other living organisms, have the ability to shape mineral crystals into unconventional morphologies and to assemble them into complex functional mineral–organic structures, an observation that inspired tremendous research efforts in scientific and technological domains. Despite these, a biochemical toolkit that accounts for the formation of the vast variety of the observed mineral morphologies cannot be identified yet. Herein, phase‐field modeling of molluscan nacre formation, an intensively studied biomineralization process, is used to identify key physical parameters that govern mineral morphogenesis. Manipulating such parameters, various nacre properties ranging from the morphology of a single mineral building block to that of the entire nacreous assembly are reproduced. The results support the hypothesis that the control over mineral morphogenesis in mineralized tissues happens via regulating the physico‐chemical environment, in which biomineralization occurs: the organic content manipulates the geometric and thermodynamic boundary conditions, which in turn, determine the process of growth and the form of the biomineral phase. The approach developed here has the potential of providing explicit guidelines for the morphogenetic control of synthetically formed composite materials.

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Dermatopontin, a shell matrix protein gene from pearl oyster Pinctada martensii, participates in nacre formation

Cited by 38 publications

References 20 publications

Polymorphs, Proteins, and Nucleation Theory: A Critical Analysis

Polymorphs, Proteins, and Nucleation Theory: A Critical Analysis

A novel nacre matrix protein hic24 in Hyriopsis cumingii is essential for calcium carbonate nucleation and involved in pearl formation

Physical Phenomena Governing Mineral Morphogenesis in Molluscan Nacre

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