Dermatophagoides pteronyssinus-induced pro-inflammatory responses mediated via STAT3 and NF-kappaB signaling pathways in human bronchial epithelial cells – Inhibitory effects of Lafoensia pacari and ellagic acid

Souza, Ariani Impieri de; Pereira, Aline Beatriz Mahler; Oliveira, Jhony Robison de; Ramalho, Luciana Santos; Souza, Henrique Ismarsi de; Nascimento, Andréa M. A.; Uddin, Mohib; Pereira, Paulo Sérgio; Teixeira, David Nascimento Silva; Silva, Paulo Roberto da; Albuquerque, Deijanira; Faccioli, Lúcia Helena; Rogério, Alexandre de Paula

doi:10.1016/j.jphs.2020.01.004

Cited by 9 publications

(8 citation statements)

References 37 publications

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“…A series of studies [12,24,30] have proved that C. perfringens challenge can cause an up-regulation on pro-in ammatory mediator genes in the intestine of broilers, including TNF-α, IL-1β, IL-8, TGF-β, IFN-γ, and iNOS. Meanwhile, the alleviating effect of EA on in ammatory mediators, including TNF-α, IL-1β, IL-8, and iNOS has been widely reported in mice or rats [15,16], which are in line with our results and further indicated that EA reduced in ammatory mediators in broilers may be through NF-κB and STAT signaling pathways. However, the C. perfringens challenge or dietary EA levels show no signi cant effect on TGF-β and IFN-γ in our results, which may be related to the difference on C. perfringens strains and frequency of the challenge.…”

Section: Cecal Microbiotasupporting

confidence: 90%

“…Ellagic acid (EA) is a chromene-dione derivative (2,3,7,8tetrahydroxy-chromeno [5,4,3-cde]chromene-5,10-dione; C14H6O8) extracted from various fruits, nuts, vegetables, and herbs [13]. EA possesses numerous pharmacological activities, including antioxidant activities [14], anti-in ammatory activities [15,16], anti-cancer activities [13], and anti-metabolic syndrome activities [17]. In rats, EA exerted anti-in ammatory and antioxidant functions against streptozotocin -induced diabetic nephropathy via reduced the activation of NF-κB and increased the nuclear translocation of Nrf2 to up-regulate GSH, γ-GCL, and SOD activities [18].…”

Section: Introductionmentioning

confidence: 99%

“…In rats, EA exerted anti-in ammatory and antioxidant functions against streptozotocin -induced diabetic nephropathy via reduced the activation of NF-κB and increased the nuclear translocation of Nrf2 to up-regulate GSH, γ-GCL, and SOD activities [18]. Meanwhile, the alleviating effect of EA on in ammatory mediators has also been widely reported in mice or rats, including TNF-α, IL-1β, IL-6, IL-8, and iNOS, through TLRs, NF-κB, and STAT signaling pathways [15,16,19]. In addition, EA can alter intestinal microbiota composition and be transformed to urolithins, possessing the potential protection against oxidative stresses and in ammatory diseases in gastrointestine [20].…”

Section: Introductionmentioning

confidence: 99%

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Dietary Ellagic Acid Ameliorated Clostridium Perfringens-Induced Subclinical Necrotizing Enteritis In Broilers Via Regulating Inflammation Signaling Pathways And Cecal Microbiota to Inhibit Intestinal Barrier Damage

Tang¹,

Zhang²,

Wang³

et al. 2021

Preprint

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Background: Subclinical necrotizing enteritis (SNE) is a common intestinal disease caused by Clostridium perfringens in broilers, which cause chronic intestinal damage, affect the digestion and absorption of nutrients, and reduce production performance. Ellagic acid (EA) has been reported to have antioxidant and anti-inflammatory properties in many aspects. This study was conducted to evaluate the effect and mechanism of EA in relieving subclinical necrotizing enteritis in broilers induced by C. perfringens. Results: C. perfringens challenge decreased body weight (BW), average daily gain (ADG); jejunal villi height/crypt depth (V/C); the activity of catalase (CAT), and the mRNA expression of zonula occludens 1 (ZO-1) in jejunum mucosa of broilers. While it increased feed conversion ratios (FCR); jejunal crypt depth (CD); activities of myeloperoxidase (MPO) and diamine oxidase (DAO), as well as concentrations of interleukin 6 (IL-6), C-reactive protein (CRP), and procalcitonin (PCT) in serum; activities of inducible nitric oxide synthase (iNOs) and lysozyme (LZM), the concentration of malondialdehyde (MDA), and the mRNA expression of claudin-2, TNF-α, IL-1β, TLR4, TLR2, NF-κB, JAK3, STAT6 and iNOs in jejunum mucosa of broilers. Dietary EA supplement relieved these adverse effects, and heightened jejunal villi height (VH); the concentration of D-xylose in plasma; activities of superoxide dismutase (SOD), and the mRNA expression of occludin in jejunum mucosa of broilers. The alpha diversity of cecal microbiota exhibited dietary EA supplement increased observed species and Shannon index. C. perfringens challenge increased the relative abundance of Firmicutes and decreased the relative abundance of Desulfobacterota. Similarly, EA increased relative abundance of Firmicutes. LEfSe analysis showed that C. perfringens challenge harmed the cecal microbiota of broiler chickens, dietary EA supplementation led to a small beneficial effect, while the simultaneous effect of them seems to stimulate the immune function of broilers and made broilers possess a better cecal microbiota. Conclusions: Dietary EA ameliorated C. perfringens-induced SNE in broilers via regulating jejunal inflammation signaling pathways TLR/NF-κB and JAK3/STAT6, relieving jejunal oxidative stress, and balancing cecal microbiota to inhibit intestinal barrier damage, prevent systemic inflammatory response, and improve nutrient absorption capacity, finally protect and enhance growth performance of broilers.

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Section: Cecal Microbiotasupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dietary Ellagic Acid Ameliorated Clostridium Perfringens-Induced Subclinical Necrotizing Enteritis In Broilers Via Regulating Inflammation Signaling Pathways And Cecal Microbiota to Inhibit Intestinal Barrier Damage

Tang¹,

Zhang²,

Wang³

et al. 2021

Preprint

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“…23,24 Ellagic acid another substantially identified phytometabolite belonging to the tannin class of polyphenols also exhibits scientifically proven anti-oxidant, anti-microbial and anti-inflammatory activities. [25][26][27] Roots of Glycyrrhiza glabra in DHP served as a rich source of glycyrrhizin which was also identified through both, HPTLC and UHPLC techniques. Various in vitro and in vivo models determined that glycyrrhizin cures asthma by inhibiting the heightened inflammatory response via PI3K/Akt/GSK3β pathway.…”

Section: Discussionmentioning

confidence: 99%

Divya-Herbal-Peya Decoction Harmonizes the Inflammatory Response in Lipopolysaccharide-Induced Zebrafish Model

Balkrishna¹,

Lochab²,

Joshi³

et al. 2021

JEP

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Background: Divya-Herbal-Peya (DHP) is a plant-based decoction containing fourteen herbs in precise quantities; usually prescribed by the practitioners in Ayurveda to alleviate stress and minimize the exasperating symptoms of recurring infections. Our study aims to provide an experimental validation to the immunomodulatory properties of DHP. Methods: Physico-chemical analysis of DHP was performed to evaluate the presence of secondary metabolites. The phytochemicals were then identified and quantitated through HPTLC, UHPLC, and GC-MS techniques. To address the scientific rationale behind DHP, lipopolysaccharide (LPS) was intraperitoneally injected in adult zebrafish to develop inflammatory response. Following LPS-induction, abnormality in locomotory behaviour was determined by evaluating the relative swim velocity and the rate of turning in experimental zebrafish. Pathophysiological effects were determined through opercular frequency, behavioural fever, and caudal fin damage. LPS-mediated inflammation was measured of pro-inflammatory cytokines, TNFα, IL-6, and IL-1β expression in the serum of study animals, by RT-PCR. Results: Our study phytochemically characterized and ascertained the presence of glycyrrhizin, rosmarinic acid, gingerol, cinnamic acid, protocatechuic acid, gallic acid, ellagic acid, piperine and cinnamaldehyde in DHP decoction. LPS induced aberrant locomotory patterns, behavioural fever and caudal fin damage in zebrafish. A significant increase in gene expression levels of pro-inflammatory cytokines, TNFα, IL-6, and IL-1β was also determined. However, these locomotory deviations and behavioural fever were negligible in zebrafish groups preadministered either with DHP in a dose dependent manner or dexamethasone (DEX). The altered opercular rate, caudal fin damage and elevated transcription levels of pro-inflammatory genes upon LPS-induction were averted in groups pre-treated with DHP and DEX. Conclusion: DHP prophylactically prevented the LPS-induced abnormal behaviour and inflammation-related pathophysiology in zebrafish. Immunomodulatory properties of DHP may not have therapeutic intervention, but do confer nutraceutical health benefits against mild infections.

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“…Lindl., and Lepidium apetalum Willd. can reduce the production of inflammatory cytokines and oxidative stress to prevent LPS-induced ALI in mice through the NF-κB pathway (Cornélio Favarin et al, 2013;Huang et al, 2013;Lee et al, 2014b;Shu et al, 2014;Wang et al, 2014;Chi et al, 2016;Li et al, 2016;Luan et al, 2016;Liu et al, 2018a;Qian et al, 2019;Júlio de Souza et al, 2020;Ren et al, 2021). TLR4/MYD88, an upstream player of the NF-κB pathway, mediates the inflammation and ALI.…”

Section: Inflammation and Alimentioning

confidence: 99%

Therapeutic Effects and Molecular Mechanisms of Bioactive Compounds Against Respiratory Diseases: Traditional Chinese Medicine Theory and High-Frequency Use

Wang

Ding

et al. 2021

Front. Pharmacol.

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Respiratory diseases, especially the pandemic of respiratory infectious diseases and refractory chronic lung diseases, remain a key clinical issue and research hot spot due to their high prevalence rates and poor prognosis. In this review, we aimed to summarize the recent advances in the therapeutic effects and molecular mechanisms of key common bioactive compounds from Chinese herbal medicine. Based on the theories of traditional Chinese medicine related to lung diseases, we searched several electronic databases to determine the high-frequency Chinese medicines in clinical application. The active compounds and metabolites from the selected medicines were identified using the Traditional Chinese Medicine Systems Pharmacology Database (TCMSP) by analyzing oral bioavailability and drug similarity index. Then, the pharmacological effects and molecular mechanisms of the selected bioactive compounds in the viral and bacterial infections, inflammation, acute lung injury (ALI), chronic obstructive pulmonary disease (COPD), pulmonary fibrosis, asthma, and lung cancer were summarized. We found that 31 bioactive compounds from the selected 10 common Chinese herbs, such as epigallocatechin-3-gallate (EGCG), kaempferol, isorhamnetin, quercetin, and β-sitosterol, can mainly regulate NF-κB, Nrf2/HO-1, NLRP3, TGF-β/Smad, MAPK, and PI3K/Akt/mTOR pathways to inhibit infection, inflammation, extracellular matrix deposition, and tumor growth in a series of lung-related diseases. This review provides novel perspectives on the preclinical study and clinical application of Chinese herbal medicines and their bioactive compounds against respiratory diseases.

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Dermatophagoides pteronyssinus-induced pro-inflammatory responses mediated via STAT3 and NF-kappaB signaling pathways in human bronchial epithelial cells – Inhibitory effects of Lafoensia pacari and ellagic acid

Cited by 9 publications

References 37 publications

Dietary Ellagic Acid Ameliorated Clostridium Perfringens-Induced Subclinical Necrotizing Enteritis In Broilers Via Regulating Inflammation Signaling Pathways And Cecal Microbiota to Inhibit Intestinal Barrier Damage

Dietary Ellagic Acid Ameliorated Clostridium Perfringens-Induced Subclinical Necrotizing Enteritis In Broilers Via Regulating Inflammation Signaling Pathways And Cecal Microbiota to Inhibit Intestinal Barrier Damage

Divya-Herbal-Peya Decoction Harmonizes the Inflammatory Response in Lipopolysaccharide-Induced Zebrafish Model

Therapeutic Effects and Molecular Mechanisms of Bioactive Compounds Against Respiratory Diseases: Traditional Chinese Medicine Theory and High-Frequency Use

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