Dermatology and the American Experience in Space

Burgdorf, Walter H. C.; Hoenig, Leonard J.

doi:10.1001/jamadermatol.2014.2557

Cited by 10 publications

(13 citation statements)

References 2 publications

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“…These new findings with NHDF indicate what may possibly occur in space. The behavior of fibroblasts under space conditions is of high interest because the astronaut’s skin is getting thinner (thinned stratum corneum, impaired barrier function, and loss of dermal elasticity) 3,107 with time. Research in this area is of high interest because of the planned space tourism adventures in the near future.…”

Section: Discussionmentioning

confidence: 99%

Morphological and Molecular Changes in Juvenile Normal Human Fibroblasts Exposed to Simulated Microgravity

Buken

Sahana

Corydon

et al. 2019

Sci Rep

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The literature suggests morphological alterations and molecular biological changes within the cellular milieu of human cells, exposed to microgravity (µ g ), as many cell types assemble to multicellular spheroids (MCS). In this study we investigated juvenile normal human dermal fibroblasts (NHDF) grown in simulated µ g (s-µ g ) on a random positioning machine (RPM), aiming to study changes in cell morphology, cytoskeleton, extracellular matrix (ECM), focal adhesion and growth factors. On the RPM, NHDF formed an adherent monolayer and compact MCS. For the two cell populations we found a differential regulation of fibronectin, laminin, collagen-IV, aggrecan, osteopontin, TIMP-1, integrin-β 1 , caveolin-1, E-cadherin, talin-1, vimentin, α-SM actin, TGF-β 1 , IL-8, MCP-1, MMP-1, and MMP-14 both on the transcriptional and/or translational level. Immunofluorescence staining revealed only slight structural changes in cytoskeletal components. Flow cytometry showed various membrane-bound proteins with considerable variations. In silico analyses of the regulated proteins revealed an interaction network, contributing to MCS growth via signals mediated by integrin-β 1 , E-cadherin, caveolin-1 and talin-1. In conclusion, s-µ g -conditions induced changes in the cytoskeleton, ECM, focal adhesion and growth behavior of NHDF and we identified for the first time factors involved in fibroblast 3D-assembly. This new knowledge might be of importance in tissue engineering, wound healing and cancer metastasis.

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Section: Discussionmentioning

confidence: 99%

Morphological and Molecular Changes in Juvenile Normal Human Fibroblasts Exposed to Simulated Microgravity

Buken

Sahana

Corydon

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…It is known that space flight exposes the skin to microgravity and cosmic radiation [3]. One study, using multiphoton tomography, demonstrated that astronauts after long-term space flights experience both thinning and loss of elasticity [3,8]. The molting time of the cells from the stratum basale toward the stratum corneum has also been shown to be increased [8].…”

Section: Epidermal Thinningmentioning

confidence: 99%

“…Another study recently performed on the ISS took measurements of the inner forearm skin before, during, and after a long-term mission [3]. Each astronaut treated one forearm with a protective cream and the other untreated forearm served as the control.…”

Section: Epidermal Thinningmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Most dermatologic complaints in space have not been of a serious nature; some are easy to explain, such as the mild contact dermatitis from biosensor electrolyte paste experienced by the commander of Apollo 12 [3]. However, other dermatologic complaints are more difficult to explain, such as descriptions of dry, itchy, and thinning skin, increased sensitivity, and/or delayed wound healing [3]. Most experts agree that although dermatologic complaints are the most commonly reported medical event, they are the least likely of medical conditions experienced in space to seriously affect the completion of the mission and crewmembers' health [4,5].…”

Section: Introductionmentioning

confidence: 99%

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