Dermatan sulfate obtained from the Phallusia nigra marine organism is responsible for antioxidant activity and neuroprotection in the neuroblastoma-2A cell lineage

Sousa, Graziele Fonseca de; Palmero, Celia Yelimar; Souza-Menezes, Jackson; Araujo, Aline Karl; Guimarães, A. O.; Barros, Cíntia Monteiro de

doi:10.1016/j.ijbiomac.2020.06.285

Cited by 11 publications

(3 citation statements)

References 42 publications

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“…Neuro-2A cell lineage [35] Polysaccharide (CPA-1 and CPB- Natural macromolecules have interesting physicochemical characteristics such as a richness of functional groups that allow chemical modification and functionalization. Moreover, natural polysaccharides have other beneficial properties including solubility in water, biocompatibility, high stability and low toxicity [78].…”

Section: Preventive (Antioxidant Activity and Neuroprotection)mentioning

confidence: 99%

“…Habaike et al [149] aimed to identify the protective roles of various components in Fomes officinalis Ames polysaccharides (FOAPs) in neuronal cells. Various concentrations of FOAPs were applied to neuronal cells two hours prior to direct exposure to a β-amyloid protein fragment [25][26][27][28][29][30][31][32][33][34][35]. The AD disease model of neuronal cells was developed at cellular level using Aβ25-35 [149].…”

Section: Antioxidant Activity Of Polysaccharidesmentioning

confidence: 99%

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Natural Polysaccharides as Preventive and Therapeutic Horizon for Neurodegenerative Diseases

et al. 2021

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Neurodegenerative diseases are a serious and widespread global public health burden amongst aging populations. The total estimated worldwide global cost of dementia was US$818 billion in 2015 and has been projected to rise to 2 trillion US$ by 2030. While advances have been made to understand different neurodegenerative disease mechanisms, effective therapeutic strategies do not generally exist. Several drugs have been proposed in the last two decades for the treatment of different types of neurodegenerative diseases, with little therapeutic benefit, and often with severe adverse and side effects. Thus, the search for novel drugs with higher efficacy and fewer drawbacks is an ongoing challenge in the treatment of neurodegenerative disease. Several natural compounds including polysaccharides have demonstrated neuroprotective and even therapeutic effects. Natural polysaccharides are widely distributed in plants, animals, algae, bacterial and fungal species, and have received considerable attention for their wide-ranging bioactivity, including their antioxidant, anti-neuroinflammatory, anticholinesterase and anti-amyloidogenic effects. In this review, we summarize different mechanisms involved in neurodegenerative diseases and the neuroprotective effects of natural polysaccharides, highlighting their potential role in the prevention and therapy of neurodegenerative disease.

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Section: Preventive (Antioxidant Activity and Neuroprotection)mentioning

confidence: 99%

Section: Antioxidant Activity Of Polysaccharidesmentioning

confidence: 99%

Natural Polysaccharides as Preventive and Therapeutic Horizon for Neurodegenerative Diseases

et al. 2021

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“…These ascidian-derived GAGs are considered analogous to mammalian GAGs, playing essential roles in different biological processes such as tissue integrity, fertilization, infection, inflammation, cell growth, and others [31][32][33]. Therefore, ascidians are seen today as a promising natural source for the production of polysaccharides with antitumoral, anticoagulant, antimicrobial, antioxidant, neuritogenic, neuroprotective, and anti-inflammatory therapeutic properties [34][35][36][37][38].…”

Section: Introductionmentioning

confidence: 99%

Extraction, Isolation, Characterization, and Biological Activity of Sulfated Polysaccharides Present in Ascidian Viscera Microcosmus exasperatus

Bento,

Maciel,

Bezerra

et al. 2023

Pharmaceuticals

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Ascidians are marine invertebrates that synthesize sulfated glycosaminoglycans (GAGs) within their viscera. Ascidian GAGs are considered analogues of mammalian GAGs and possess great potential as bioactive compounds, presenting antitumoral and anticoagulant activity. Due to its worldwide occurrence and, therefore, being a suitable organism for large-scale mariculture in many marine environments, our main objectives are to study Microcosmus exasperatus GAGs regarding composition, structure, and biological activity. We also aim to develop efficient protocols for sulfated polysaccharides extraction and purification for large-scale production and clinical applications. GAGs derived from M. exasperatus viscera were extracted by proteolytic digestion, purified by ion-exchange liquid chromatography, and characterized by agarose gel electrophoresis and enzymatic treatments. Anticoagulant activity was evaluated by APTT assays. Antitumoral activity was assessed in an in vitro model of tumor cell culture using MTT, clonogenic, and wound healing assays, respectively. Our results show that M. exasperatus presents three distinct polysaccharides; among them, two were identified: a dermatan sulfate and a fucosylated dermatan sulfate. Antitumoral activity was confirmed for the total polysaccharides (TP). While short-term incubation does not affect tumor cell viability at low concentrations, long-term TP incubation decreases LLC tumor cell growth/proliferation at different concentrations. In addition, TP decreased tumor cell migration at different concentrations. In conclusion, we state that M. exasperatus presents great potential as an alternative GAG source, producing compounds with antitumoral properties at low concentrations that do not possess anticoagulant activity and do not enhance other aspects of malignancy, such as tumor cell migration. Our perspectives are to apply these molecules in future preclinical studies for cancer treatment as antitumoral agents to be combined with current treatments to potentiate therapeutic efficacy.

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Chemistry and Function of Glycosaminoglycans in the Nervous System

Schwartz

Domowicz

2022

Advances in Neurobiology

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Dermatan sulfate obtained from the Phallusia nigra marine organism is responsible for antioxidant activity and neuroprotection in the neuroblastoma-2A cell lineage

Cited by 11 publications

References 42 publications

Natural Polysaccharides as Preventive and Therapeutic Horizon for Neurodegenerative Diseases

Natural Polysaccharides as Preventive and Therapeutic Horizon for Neurodegenerative Diseases

Extraction, Isolation, Characterization, and Biological Activity of Sulfated Polysaccharides Present in Ascidian Viscera Microcosmus exasperatus

Chemistry and Function of Glycosaminoglycans in the Nervous System

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