Dermal toxicity, eye and dermal irritation and skin sensitization evaluation of a new formulation of Bacillus thuringiensis var israelensis SH-14

Arteaga, M.E.; Mancebo, A.; Molier, T.; Gomez, Daniel Gomez; Gonzalez, Celia; Bada, A.M.; González, B.; Rojas, Nidia; Rodríguez, Graciela

doi:10.1016/j.yrtph.2013.12.003

Cited by 8 publications

(4 citation statements)

References 11 publications

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“…The formulation was found to be safe to non-target organisms found in association with mosquito larvae in the aquatic habitats. This observation reinforces the results of several earlier studies conducted with Bti 15 27 28 . This study showed that the carrier material and additive, namely FA and CMC used in this formulation, as well as the active ingredient, Bti, were safe on mammalian systems.…”

Section: Discussionsupporting

confidence: 92%

Fly ash-based water dispersible powder formulation of Bacillus thuringiensis var. israelensis: Development & laboratory evaluation against mosquito immatures

2017

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Background & objectives:Bacillus thuringiensis var. israelensis (Bti) formulations are presently being used for insect control. In this study, a water dispersible powder (WDP) formulation using fly ash (FA) as a carrier material was developed and studied for its activity against the larval stages of major mosquito vector species.Methods:An indigenous isolate Bti (Vector Control Research Centre B17) was mass produced using a 100 l fermentor in soya-based medium. The bacterial biomass was mixed with lignite FA and made into WDP formulations. The most effective formulation was used for determining 50 per cent lethal concentration (LC50) against the larval stages of major mosquito vector species, effect on non-target organisms and mammalian systems using standard protocols.Results:Sixteen types of WDP formulations were prepared, of which the formulation containing bacterial biomass, FA and carboxymethyl cellulose was found to be the most effective. The LC50 values of the formulation against Culex quinquefasciatus, Aedes aegypti and Anopheles stephensi larvae were 0.0417, 0.0462 and 0.1091 mg/l, respectively. The formulation was found to be safe to non-target organisms found associated with the mosquito larval stages and also to mammalian systems.Interpretation & conclusions:The study shows that FA can be effectively used to replace commercially available carrier materials used in biopesticidal formulations.

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Section: Discussionsupporting

confidence: 92%

Fly ash-based water dispersible powder formulation of Bacillus thuringiensis var. israelensis: Development & laboratory evaluation against mosquito immatures

2017

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“…Arteaga et al demonstrated that new formulation of Bti SH-14 is not acutely toxic via dermal route and has no dermal irritation or hypersensitivity but has low eye irritation in the experimented rabbits. 33 Wilcks et al performed an oral administration of B. thuringiensis in rats. 34 Finally, these bacteria were detected at high density in fecal and intestinal samples even 2 weeks following the last dosage.…”

Section: Discussionmentioning

confidence: 99%

Toxicological Examinations Following Oral Administration of Bacillus thuringiensis var kurstaki Biological Insecticide in Wistar Rats

Larki

Zayerzadeh²,

Harzandi

et al. 2019

J Apple Biotechnol Rep

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Introduction: Bacillus thuringiensis var kurstaki is one of the best known biological insecticide which is used extensively in the world. This biopesticide has been broadly used against important insect pests and vectors of animals and humans. However, the controversial data was published to date considering toxicological studies on non-target species are urgent to determine probable adverse effects and risk assessment of this biopesticide. In this research, histopathological changes, hematological and some biochemical factors were evaluated following the single dose oral administration of B. thuringiensis in rats. Materials and Methods: Twelve Wistar rats were randomly divided into 2 groups including experimental and control groups. Animals were treated orally by gavage to single dose of sub-lethal dose (5000 mg/kg) of B. thuringiensis suspension. Finally, hematological factors (WBC, RBC, Hb, HCT, MCV, MCH, MCHC and PLT), some biochemical parameters (ALT, AST, ALP, BUN and Cr) and histopathological observations were evaluated. Results: The results demonstrated that oral administration of high dose of B. thuringiensis biopesticide induced some pathological complications including congestion and inflammation in vital organs of rats such as liver, heart, lung and kidney. It also caused hematological abnormalities and biochemical alterations in the experimental animals group. Conclusions: According to the findings, it can be concluded that high dose of B. thuringiensis biopesticide can induce toxicity in rats. Therefore, further investigations including subacute and chronic are recommended.

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“…Frente aos riscos associados ao uso de pesticidas sintéticos, há um crescente interesse em alternativas menos agressivas ao ambiente para o controle de pestes na agricultura (Pino-Otín et al, 2019). Neste cenário, os biopesticidas aparecem no mercado como uma alternativa menos prejudicial ao meio ambiente (Cruz et al, 2004;Oliveira-Filho, 2008;Artega et al, 2014), pois dispõe de maior especificidade com o organismo-alvo, afetando um número menor de organismos não-alvo (Glare et al, 2012;Villaverde et al, 2014).…”

Section: Introductionunclassified

Avaliação de toxicidade de biopesticida comercial à base de Bacillus thuringiensis em tilapia-do-Nilo, Oreochromis niloticus

Simão

Silva

Soares

et al. 2021

RSD

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Os biopesticidas são usados como uma alternativa ao uso de pesticidas químicos sintéticos. As formulações que contêm esporos de Bacillus thuringiensis (Bt) são amplamente utilizados, entretanto, pouco se sabe dos efeitos na biota aquática. O objetivo do estudo foi avaliar os efeitos genotóxicos e histopatológicos de uma concentração subletal de um biopesticida comercial à base de Bt em Oreochromis niloticus. Os peixes foram divididos (n = 10) em um grupo controle (GC) e um grupo exposto (GBt) na concentração de 60 mg L-1 do biopesticida Dipel® WP por 48 horas. Após a exposição, amostras de sangue, brânquias, fígado e rim foram coletadas para análise de biomarcadores de toxicidade. Os resultados demonstram que uma exposição aguda ao Bt induz o aumento de núcleos eritrocitários com morfologia segmentada. Não foi observado a indução de micronúcleos. As análises histopatológicas demonstraram que em 48 horas de exposição, no GBt, houve uma diminuição na frequência de hepatócitos hipertrofiados. Entretanto, o biopesticida não comprometeu a morfologia dos tecidos em toxicidade aguda. Os pesticidas de modo geral, são aplicados constantemente e regulamentações de uso não são amplamente seguidas, assim, nossos resultados sugerem que concentrações subletais podem induzir efeitos quando expostos de modo agudo. Os biopesticidas à base de Bt em baixas concentrações parecem ser pouco tóxicos aos peixes.

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Dermal toxicity, eye and dermal irritation and skin sensitization evaluation of a new formulation of Bacillus thuringiensis var israelensis SH-14

Cited by 8 publications

References 11 publications

Fly ash-based water dispersible powder formulation of Bacillus thuringiensis var. israelensis: Development & laboratory evaluation against mosquito immatures

Fly ash-based water dispersible powder formulation of Bacillus thuringiensis var. israelensis: Development & laboratory evaluation against mosquito immatures

Toxicological Examinations Following Oral Administration of Bacillus thuringiensis var kurstaki Biological Insecticide in Wistar Rats

Avaliação de toxicidade de biopesticida comercial à base de Bacillus thuringiensis em tilapia-do-Nilo, Oreochromis niloticus

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