Dermal fibroblast from superficial layers of pig skin exhibits more proliferative capacity than that from deep layers

Zuo, Yanhai; Lee, Yi; Lu, Shuliang

doi:10.1016/j.jtv.2021.12.001

Cited by 1 publication

(1 citation statement)

References 24 publications

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“…Since the pig exhibits great genetic, physiological, and anatomical similarities with human, porcine fibroblasts are reasonably considered as potentially optimal alternatives to human fibroblasts in various studies [ 10 , 11 , 12 ]. Sood et al suggested that the pig is a promising model for the study of human pathologic scarring as the in vitro behavior of porcine dermal fibroblasts closely mirrored that of human hypertrophic scar fibroblasts under similar conditions [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluating the Effects of Cryopreservation on the Viability and Gene Expression of Porcine-Ear-Skin Fibroblasts

Cao

Xie

Wang

et al. 2023

Genes

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Owing to the inherent heterogeneity and plasticity of fibroblasts, they are considered as the conventional biological resources for basic and clinical medical research. Thus, it is essential to generate knowledge about the establishment of fibroblast cultures and the effects of cryopreservation processes on their biological characteristics. Since the pig (Sus scrofa) possesses numerous genetic, physiological, and anatomical similarities with humans, porcine fibroblasts are naturally regarded as useful analogues of human fibroblasts. Nonetheless, less attention has been given to the alterations in viability and gene expression of cryopreserved porcine fibroblasts. In this study, we aimed to obtain fibroblasts from porcine ear skin and evaluate the effects of cryopreservation on the cell survival, proliferation, and gene expression profiles of the fibroblasts by trypan-blue-staining assay, Cell Counting kit-8 (CCK-8) assay, and RNA-sequencing analysis, respectively. Our results suggested that morphologically stable fibroblast cultures can be constructed from pig-ear skin. The post-thaw survival rate of the cryopreserved fibroblasts at 0 h and 24 h was over 90%. The proliferative activity of the cryopreserved fibroblasts was similar to that of the non-cryopreserved fibroblasts after 7 days of in vitro culture, which suggested that cryopreservation did not influence the viability. The RNA-sequencing analysis indicated that this should be attributed to the 867 differentially expressed genes (DGEs) identified, which are involved in molecular process related to cell recovery and survival after cryo-stimulation. In addition, eight important DEGs BMP2, GDF15, EREG, AREG, HBEGF, LIF, IL-6, and HOX-7 could potentially be applied to improve the efficiency of fibroblast cryopreservation, but comprehensive and systematic studies on understanding the underlying mechanisms responsible for their modulatory roles are urgently needed.

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