Dermal Fibroblast CCN1 Expression in Mice Recapitulates Human Skin Dermal Aging

Quan, Taihao; Xiang, Yong‐Bing; Liu, Yingchun; Qin, Zhaoping; Yang, Yan; Bou‐Gharios, George; Voorhees, John J.; Dlugosz, Andrzej A.; Fisher, Gary J.

doi:10.1016/j.jid.2020.07.019

Cited by 12 publications

(16 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3A). Moreover, epidermal expression of the antiaging collagen 17A/BP180, which is critical to protect epithelial stem cells from aging ( 19 , 20 ), significantly increased along the basement membrane of OiY human epidermis in vivo compared to its low expression level in pre-engraftment aged human skin and OiO xenotransplants (Fig. 3B).…”

Section: Resultsmentioning

confidence: 99%

“…3, D and E). This is important because thick collagen bundles decline in both number and diameter during extrinsic and intrinsic skin aging ( 20 ). Moreover, the histochemically assessed abundance of elastin expression, which also declines during skin aging ( 24 ), was significantly higher in OiY compared with OiO and in pre-engraftment skin ( P < 0.05) (fig.…”

Section: Resultsmentioning

confidence: 99%

“…Human skin samples were analyzed by histochemistry, IHC, and IF microscopy as described (Fig. 1, B and D) ( 16 ) using the following established aging-associated readout parameters: β-Gal activity ( 14 ), SIRT1 ( 17 , 18 ), PGC1α ( 17 , 18 ), p16 ink4a ( 15 ), NRF2 ( 71 ), HO-1 ( 22 ), peroxireduxin ( 23 ), GSR ( 23 ), MMP1 ( 20 ), MTCO-1 ( 16 ), thick/thin collagen bundles ( 20 ), collagen 17A/BP180 ( 19 ), elastin ( 24 ), and HABP ( 25 ).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Human organ rejuvenation by VEGF-A: Lessons from the skin

et al. 2022

View full text Add to dashboard Cite

Transplanting aged human skin onto young SCID/beige mice morphologically rejuvenates the xenotransplants. This is accompanied by angiogenesis, epidermal repigmentation, and substantial improvements in key aging-associated biomarkers, including ß-galactosidase, p16 ink4a , SIRT1, PGC1α, collagen 17A, and MMP1. Angiogenesis- and hypoxia-related pathways, namely, vascular endothelial growth factor A (VEGF-A) and HIF1A, are most up-regulated in rejuvenated human skin. This rejuvenation cascade, which can be prevented by VEGF-A–neutralizing antibodies, appears to be initiated by murine VEGF-A, which then up-regulates VEGF-A expression/secretion within aged human skin. While intradermally injected VEGF-loaded nanoparticles suffice to induce a molecular rejuvenation signature in aged human skin on old mice, VEGF-A treatment improves key aging parameters also in isolated, organ-cultured aged human skin, i.e., in the absence of functional skin vasculature, neural, or murine host inputs. This identifies VEGF-A as the first pharmacologically pliable master pathway for human organ rejuvenation in vivo and demonstrates the potential of our humanized mouse model for clinically relevant aging research.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Human organ rejuvenation by VEGF-A: Lessons from the skin

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The main barrier to transdermal delivery is the stratum corneum on the surface of the skin, for which a dense brick-wall structure blocks the entry of most substances . Therefore, in vitro penetration experiments using mouse skin, similar to human skin, can effectively predict in vivo penetration efficiency. , UV spectrophotometry revealed that both FITC-labeled GHK-Cu/PBS and GHK-Cu/BMa had clear, detectable absorption peaks at 485 nm. CLSM revealed a 3.57-fold increase in FITC content in the mouse skin after delivery by BMa (Figure a,b).…”

Section: Resultsmentioning

confidence: 99%

Noninvasive and Efficient Peptide Delivery by a Novel Biocompatible Ionic Liquid

Liu

Zhang

Lü³

et al. 2022

ACS Sustainable Chem. Eng.

View full text Add to dashboard Cite

The application of bioactive polypeptides has become a challenge in the pharmaceutical industry. Copper glycyl-histidine-tripeptide (GHK-Cu) exerts various biological effects, including antioxidation, promotion of cell proliferation, and enhancement of repair. However, its clinical application is restricted by its hydrophilicity and severe demands with respect to the delivery conditions. Therefore, for the purpose of its application and of resolving the existing issues of GHK-Cu, we designed and developed a new local delivery system based on green ionic liquids (ILs) by combining the density functional theory (DFT) and adequate experiments. The system integrates the advantages of the ILs and GHK-Cu, which not only stabilizes GHK-Cu and greatly enhances its transdermal absorption rate but also has a synergistic amplification effect. Cell and animal experiments show encouraging effects on cell proliferation, antioxidation, and collagen synthesis, whereas human efficacy tests show the benefits of improving skin repair ability and antiaging, antiwrinkle, and moisturizing properties. The bio-based ILs system developed in this study provides a simple and efficient method for the clinical application of GHK-Cu and a novel method for the application of polypeptides.

show abstract

“…Although the skin is incredibly durable and has an enormous regenerative capacity, it cannot escape aging due to the turnover rate of epidermal cells ( Rinnerthaler et al, 2015 ). Skin aging leads to, among other effects, deleterious alterations in the structure and function of dermal collagen ( Quan et al, 2021 ). With aging, the structure and function of the skin barrier changes.…”

Section: Dermatological Diseasesmentioning

confidence: 99%

Calcium Channels: Noteworthy Regulators and Therapeutic Targets in Dermatological Diseases

Wang

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

Dysfunctional skin barrier and impaired skin homeostasis may lead to or aggravate a series of dermatologic diseases. A large variety of biological events and bioactive molecules are involved in the process of skin wound healing and functional recovery. Calcium ions (Ca2+) released from intracellular stores as well as influx through plasma membrane are essential to skin function. Growing evidence suggests that calcium influx is mainly regulated by calcium-sensing receptors and channels, including voltage-gated, transient potential receptor, store-operated, and receptor-operated calcium channels, which not only maintain cellular Ca2+ homeostasis, but also participate in cell proliferation and skin cell homeostasis through Ca2+-sensitive proteins such as calmodulin (CaM). Furthermore, distinct types of Ca2+ channels not merely work separately, they may work concertedly to regulate cell function. In this review, we discussed different calcium-sensing receptors and channels, including voltage-gated, transient receptor potential, store-operated, and receptor-operated calcium channels, particularly focusing on their regulatory functions and inherent interactions as well as calcium channels-related reagents and drugs, which is expected to bridge basic research and clinical applications in dermatologic diseases.

show abstract

Dermal Fibroblast CCN1 Expression in Mice Recapitulates Human Skin Dermal Aging

Cited by 12 publications

References 42 publications

Human organ rejuvenation by VEGF-A: Lessons from the skin

Human organ rejuvenation by VEGF-A: Lessons from the skin

Noninvasive and Efficient Peptide Delivery by a Novel Biocompatible Ionic Liquid

Calcium Channels: Noteworthy Regulators and Therapeutic Targets in Dermatological Diseases

Contact Info

Product

Resources

About