Dermal Cell Damage Induced by Topical Application of Non-Steroidal Anti-Inflammatory Drugs is Suppressed by Trehalose Co-Lyophilization in &lt;i&gt;Ex Vivo&lt;/i&gt; Analysis

Kayasuga-Kariya, Yuko; Iwanaga, Shintaroh; Fujisawa, Akio; Lin, Lee-Shuan; Suzuki, Shigeki; Chung, Ung‐il; Sasaki, Nobuo; Shimohata, Nobuyuki; Mochizuki, Manabu

doi:10.1292/jvms.12-0502

Cited by 3 publications

(2 citation statements)

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“…Although trehalose has been shown to reduce UVB-induced corneal damage when applied topically to the eye (19), its use as a skin photoprotective agent has been limited by its poor permeability (25), which was improved in the present study by loading liposomes. Notably, the main results of the study indicate that, compared to other common photoprotective compounds, trehalose-loaded liposomes showed the highest efficacy in reducing the levels of the three UVB-associated markers following experimental irradiation of HaCaT cells.…”

Section: Discussionmentioning

confidence: 93%

Protective effect of trehalose-loaded liposomes against UVB-induced photodamage in human keratinocytes

Emanuele¹,

Bertona²,

Sanchís-Gomar

et al. 2014

Biomedical Reports

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Abstract. Trehalose, a naturally occurring non-reducing disaccharide, is known to act as a major protein stabilizer that can reduce ultraviolet B (UVB)-induced corneal damage when topically applied to the eye. However, due to the low skin permeability of trehalose, which makes the development of topical formulations difficult, its use as a skin photoprotective agent has been limited. Previous findings demonstrated that liposomes may significantly improve the intracellular delivery of trehalose. Therefore, the present study aimed to assess the protective effects of trehalose-loaded liposomes against UVB-induced photodamage using the immortalized human keratinocyte cell line, HaCaT. The effects were also compared to those of the common skin photoprotective compounds, including L-carnosine, L-(+)-ergothioneine, L-ascorbic acid and DL-α-tocopherol. The levels of cyclobutane pyrimidine dimers, 8-hydroxy-2'-deoxyguanosine and protein carbonylation in HaCaT cells were used as biological markers of UVB-induced damage. Compared to other compounds, trehalose-loaded liposomes showed the highest efficacy in reducing the levels of the three markers following UVB irradiation of HaCaT cells (all P<0.001 when compared to each of the four other photoprotective compounds). Therefore, these findings indicate that there may be a clinical application for trehalose-loaded liposomes, and further studies should be performed to assess the potential usefulness in skin photoprotection and the prevention of non-melanoma skin cancer.

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Section: Discussionmentioning

confidence: 93%

Protective effect of trehalose-loaded liposomes against UVB-induced photodamage in human keratinocytes

Emanuele¹,

Bertona²,

Sanchís-Gomar

et al. 2014

Biomedical Reports

View full text Add to dashboard Cite

show abstract

“…In this issue of Digestive Diseases and Sciences, Lin et al [5] report that the oral administration of co-lyophilized aspirin/trehalose (Lyo A/T) significantly reduced the occurrence of gastric injury in healthy dogs compared with dogs that received aspirin alone and that the cutaneous reaction by topical NSAIDs was suppressed by Lyo A/T [6]. The mechanism by which Lyo A/T reduced gastric damage was hypothesized to occur through the suppression of mucosal cell apoptosis supported by significantly reduced DNA fragmentation, apoptotic cell frequency, and autophagy in Lyo A/T-treated cells and the gastric mucosa of rats, the latter findings confirmed with advanced methods such as the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, immunohistochemistry of cleaved caspase-3, and Raman spectroscopy scattering bands.…”

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confidence: 99%