Deriving Osteogenic Cells from Induced Pluripotent Stem Cells for Bone Tissue Engineering

Wu, Qingqing; Yang, Bo; Hu, Kevin; Cao, Cong; Man, Yi; Wang, Ping

doi:10.1089/ten.teb.2015.0559

Cited by 40 publications

(33 citation statements)

References 75 publications

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“…Several studies have also shown that bone formation could be obtained either by the direct use of iPSCs or via prior formation of embryoid bodies. 194 These cell sources seem promising, but it remains to be checked that implanted cells do not form teratomas on the long term.…”

Section: Choosing and Preparing Cells For Btementioning

confidence: 99%

Cellularizing hydrogel-based scaffolds to repair bone tissue: How to create a physiologically relevant micro-environment?

et al. 2017

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Tissue engineering is a promising alternative to autografts or allografts for the regeneration of large bone defects. Cell-free biomaterials with different degrees of sophistication can be used for several therapeutic indications, to stimulate bone repair by the host tissue. However, when osteoprogenitors are not available in the damaged tissue, exogenous cells with an osteoblast differentiation potential must be provided. These cells should have the capacity to colonize the defect and to participate in the building of new bone tissue. To achieve this goal, cells must survive, remain in the defect site, eventually proliferate, and differentiate into mature osteoblasts. A critical issue for these engrafted cells is to be fed by oxygen and nutrients: the transient absence of a vascular network upon implantation is a major challenge for cells to survive in the site of implantation, and different strategies can be followed to promote cell survival under poor oxygen and nutrient supply and to promote rapid vascularization of the defect area. These strategies involve the use of scaffolds designed to create the appropriate micro-environment for cells to survive, proliferate, and differentiate in vitro and in vivo. Hydrogels are an eclectic class of materials that can be easily cellularized and provide effective, minimally invasive approaches to fill bone defects and favor bone tissue regeneration. Furthermore, by playing on their composition and processing, it is possible to obtain biocompatible systems with adequate chemical, biological, and mechanical properties. However, only a good combination of scaffold and cells, possibly with the aid of incorporated growth factors, can lead to successful results in bone regeneration. This review presents the strategies used to design cellularized hydrogel-based systems for bone regeneration, identifying the key parameters of the many different micro-environments created within hydrogels.

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Section: Choosing and Preparing Cells For Btementioning

confidence: 99%

Cellularizing hydrogel-based scaffolds to repair bone tissue: How to create a physiologically relevant micro-environment?

et al. 2017

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“…Along these lines, Wu and colleagues state in their recent review that efficient in vitro differentiation of hiPSCs into downstream cells, such as mesenchymal stem/ stromal cells (MSCs), osteoblasts or osteocyte-like cells is necessary to limit unde- sired tumorigenesis associated with the pluripotency of hiPSCs [49]. They also give good comparisons of the current techniques utilized to confer the induction of hiPSCs into the osteogenic lineage, an evaluation of osteogenic potentials of cells derived from each technique and cells derived from different somatic origins and comparisons of hiPSC-derived MSCs and BMSCs [49].…”

Section: Human Induced Pluripotent Stem Cells (Hipscs)mentioning

confidence: 99%

“…They also give good comparisons of the current techniques utilized to confer the induction of hiPSCs into the osteogenic lineage, an evaluation of osteogenic potentials of cells derived from each technique and cells derived from different somatic origins and comparisons of hiPSC-derived MSCs and BMSCs [49].…”

Section: Human Induced Pluripotent Stem Cells (Hipscs)mentioning

confidence: 99%

Mimetic Hierarchical Approaches for Osteochondral Tissue Engineering

Gadjanski

2018

Osteochondral Tissue Engineering

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In order to engineer biomimetic osteochondral (OC) construct, it is necessary to address both the cartilage and bone phase of the construct, as well as the interface between them, in effect mimicking the developmental processes when generating hierarchical scaffolds that show gradual changes of physical and mechanical properties, ideally complemented with the biochemical gradients. There are several components whose characteristics need to be taken into account in such biomimetic approach, including cells, scaffolds, bioreactors as well as various developmental processes such as mesenchymal condensation and vascularization, that need to be stimulated through the use of growth factors, mechanical stimulation, purinergic signaling, low oxygen conditioning, and immunomodulation. This chapter gives overview of these biomimetic OC system components, including the OC interface, as well as various methods of fabrication utilized in OC biomimetic tissue engineering (TE) of gradient scaffolds. Special attention is given to addressing the issue of achieving clinical size, anatomically shaped constructs. Besides such neotissue engineering for potential clinical use, other applications of biomimetic OC TE including formation of the OC tissues to be used as high-fidelity disease/ healing models and as in vitro models for drug toxicity/efficacy evaluation are covered. Highlights Biomimetic OC TE uses "smart" scaffolds able to locally regulate cell phenotypes and dual-flow bioreactors for two sets of conditions for cartilage/ bone Protocols for hierarchical OC grafts engineering should entail mesenchymal condensation for cartilage and vascular component for bone Immunomodulation, low oxygen tension, purinergic signaling, time dependence of stimuli application are important aspects to consider in biomimetic OC TE Mimetic Hierarchical Approaches for Osteochondral Tissue Engineering Ivana GadjanskiAbstract In order to engineer biomimetic osteochondral (OC) construct, it is necessary to address both the cartilage and bone phase of the construct, as well as the interface between them, in effect mimicking the developmental processes when generating hierarchical scaffolds that show gradual changes of physical and mechanical properties, ideally complemented with the biochemical gradients. There are several components whose characteristics need to be taken into account in such biomimetic approach, including cells, scaffolds, bioreactors as well as various developmental processes such as mesenchymal condensation and vascularization, that need to be stimulated through the use of growth factors, mechanical stimulation, purinergic signaling, low oxygen conditioning, and immunomodulation. This chapter gives overview of these biomimetic OC system components, including the OC interface, as well as various methods of fabrication utilized in OC biomimetic tissue engineering (TE) of gradient scaffolds. Special attention is given to addressing the issue of achieving clinical size, anatomically shaped constructs. Besides such neotissue en...

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“…Induced pluripotent stem cells are cells that are derived from somatic adult cells and have been reprogrammed such that they resemble an embryonic-like state and are capable of indefinite proliferation and can form cells from all three germ line lineages (endoderm, ectoderm and mesoderm) 11 including bone-forming osteoblasts [12][13][14] . We have successfully generated iPSC lines from equines 15 and have developed methods to differentiate the iPSCs into osteoblasts using traditional 2D cell culture techniques 16 .…”

Section: Introductionmentioning

confidence: 99%

Biocompatible Three-Dimensional Printed Thermoplastic Scaffold for Osteoblast Differentiation of Equine Induced Pluripotent Stem Cells

Baird

Falcon

Saeed

et al. 2019

Tissue Engineering Part C: Methods

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Deriving Osteogenic Cells from Induced Pluripotent Stem Cells for Bone Tissue Engineering

Cited by 40 publications

References 75 publications

Cellularizing hydrogel-based scaffolds to repair bone tissue: How to create a physiologically relevant micro-environment?

Cellularizing hydrogel-based scaffolds to repair bone tissue: How to create a physiologically relevant micro-environment?

Mimetic Hierarchical Approaches for Osteochondral Tissue Engineering

Biocompatible Three-Dimensional Printed Thermoplastic Scaffold for Osteoblast Differentiation of Equine Induced Pluripotent Stem Cells

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