Deriving dopaminergic neurons for clinical use. A practical approach

Gonzalez, Rodolfo; Garitaonandia, Ibon; Abramihina, Tatiana; Wambua, Gerald; Ostrowska, Alina; Brock, Mathew; Noskov, Alexander; Boscolo, Francesca S.; Craw, John S.; Laurent, Louise C.; Snyder, Evan Y.; Semechkin, Ruslan

doi:10.1038/srep01463

Cited by 50 publications

(51 citation statements)

References 17 publications

(19 reference statements)

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“…For example, 55 randomly selected homozygous pESC lines could completely match 80% of Japanese patients at three important HLA loci (A, B, and DR) [21]. Moreover, human pESCs could provide β cells, fibroblasts, cardiomyocytes, and neurons in vitro [20][21][22], suggesting that it might be possible to use these cells as an optimal source of genetically matched hESCs for cell therapy in female patients.…”

Section: Introductionmentioning

confidence: 99%

Derivation of a Homozygous Human Androgenetic Embryonic Stem Cell Line

Ding

Huang

et al. 2015

Stem Cells and Development

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Human embryonic stem cells (hESCs) have long been considered as a promising source for cell replacement therapy. However, one major obstacle for the use of these cells is immune compatibility. Histocompatible human parthenogenetic ESCs have been reported as a new method for generating human leukocyte antigen (HLA)-matched hESCs. To further investigate the possibility of obtaining histocompatible stem cells from uniparental embryos, we tried to produce androgenetic haploid human embryos by injecting a single spermatozoon into enucleated human oocyte, and establish human androgenetic embryonic stem (hAGES) cell lines from androgenetic embryos. In the present study, a diploid hAGES cell line has been established, which exhibits typical features of human ESCs, including the expression of pluripotency markers, having differentiation potential in vitro and in vivo, and stable propagation in an undifferentiated state (>P40). Bisulfite sequencing of the H19, Snrpn, Meg3, and Kv imprinting control regions suggested that hAGES cells maintained to a certain extent a sperm methylation pattern. Genome-wide single nucleotide polymorphism, short tandem repeat, and HLA analyses revealed that the hAGES cell genome was highly homozygous. These results suggest that hAGES cells from spermatozoon could serve as a useful tool for studying the mechanisms underlying genomic imprinting in humans. It might also be used as a potential resource for cell replacement therapy as parthenogenetic stem cells.

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Section: Introductionmentioning

confidence: 99%

Derivation of a Homozygous Human Androgenetic Embryonic Stem Cell Line

Ding

Huang

et al. 2015

Stem Cells and Development

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“…To generate authentic midbrain DA (mDA) neurons, we adopted dual SMAD inhibition and FP induction protocols . The common features of these protocols are early activation of Sonic Hedgehog (Shh and Pur) and WNT (ChIR) signaling during neural induction from hESCs by dual inhibition of SMAD signaling (SB431542 and LDN) (Fig.…”

Section: Resultsmentioning

confidence: 99%

Immature Midbrain Dopaminergic Neurons Derived from Floor-Plate Method Improve Cell Transplantation Therapy Efficacy for Parkinson's Disease

Qiu

Liao

Chen

et al. 2017

Stem Cells Translational Medicine

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Recent reports have indicated human embryonic stem cells‐derived midbrain dopamine (mDA) neurons as proper cell resources for use in Parkinson's disease (PD) therapy. Nevertheless, no detailed and systematic study has been conducted to identify which differentiation stages of mDA cells are most suitable for transplantation in PD therapy. Here, we transplanted three types of mDA cells, DA progenitors (differentiated in vitro for 16 days [D16]), immature DA neurons (D25), and DA neurons (D35), into PD mice and found that all three types of cells showed high viability and strong neuronal differentiation in vivo. Both D25 and D35 cells showed neuronal maturation and differentiation toward TH+ cells and, accordingly, satisfactory behavioral functional recovery. However, transplanted D16 cells were less capable of producing functional recovery. These findings provide a valuable guideline for standardizing the differentiation stage of the transplantable cells used in clinical cell therapy for PD. Stem Cells Translational Medicine 2017;6:1803–1814

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“…Medium E has been used previously for embryonic stem cell culture, 15 and medium N has been used for neural stem cell culture. 16 Thus, media E and N may be able to provide a more similar environment to that found in vivo because HCECs in vivo are not in contact with serum, but instead face the aqueous humor produced by the ciliary body.…”

Section: Discussionmentioning

confidence: 99%