1992
DOI: 10.1089/ard.1992.2.267
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Deriving a 67-Nucleotidetrans-Cleaving Ribozyme from the Hepatitis Delta Virus Antigenomic RNA

Abstract: RNAs derived from the genomic and antigenomic hepatitis delta virus are capable of self-cleavage, and thus have the potential for serving as ribozymes in a trans-cleaving reaction. Because the catalytic core of such an enzymatic RNA was not evident from phylogenetic data, we took a step-wise approach to identifying the core, reducing the RNA in size, and characterizing various properties for each size class. Thus, a 186-nucleotide antigenomic RNA (termed Ag180) was found to be capable of cleaving well in 20 M … Show more

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Cited by 8 publications
(4 citation statements)
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“…Third and finally, since the RNAs can form numerous conformers in solution (Prasad et al, 1992a), the establishment of one definitive form for the RNA is at present beyond our technical abilities. We have sought in our experimental approach to present a reasonable model for the refolding, based upon the free energy measurements, and to take the global approach of preventing the proposed interaction by deletion mutagenesis.…”
Section: Resultsmentioning
confidence: 99%
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“…Third and finally, since the RNAs can form numerous conformers in solution (Prasad et al, 1992a), the establishment of one definitive form for the RNA is at present beyond our technical abilities. We have sought in our experimental approach to present a reasonable model for the refolding, based upon the free energy measurements, and to take the global approach of preventing the proposed interaction by deletion mutagenesis.…”
Section: Resultsmentioning
confidence: 99%
“…Earlier it was proposed that the biphasic nature of cleavage profiles at a given temperature might be due to the necessity of some RNAs within the population to reassort into the catalytically active conformation (Prasad et al, 1992a;Been et al, 1992). The possible exception to this was the Ag5-89 molecule, in which the entire population cleaved in under 15 s. While a structural rearrangement to achieve the active form may still be one of the rate-limiting steps in the cleavage reaction, this work suggests that other self-cleaving conformations may coexist with the most rapid form.…”
Section: Discussionmentioning
confidence: 99%
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“…Drug delivery and the nonspecific nature of antisense strategies will limit the application of this technology for the near future. Ribozymes and nonfunctioning ribozymes: Ribozymes are catalytic RNA molecules that serve as enzymes able to break RNA molecules at specific sites (63)(64)(65). Ribozymes designed for specific sites in the HCV genome may lead to decreased viral protein production.…”
Section: New Methods Of Interfering With Viral Replicationmentioning
confidence: 99%