Derivatization-based High-throughput Bioanalysis by LC-MS

Toyo’oka, Toshimasa

doi:10.2116/analsci.33.555

Cited by 41 publications

References 90 publications

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Derivatization procedures and their analytical performances for HPLC determination in bioanalysis

David

Moldoveanu

Galaon

2020

Biomedical Chromatography

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Derivatization, or chemical structure modification, is often used in bioanalysis performed by liquid chromatography technique in order to enhance detectability or to improve the chromatographic performance for the target analytes. The derivatization process is discussed according to the analytical procedure used to achieve the reaction between the reagent and the target compounds (containing hydroxyl, thiol, amino, carbonyl and carboxyl as the main functional groups involved in derivatization). Important procedures for derivatization used in bioanalysis are in situ or based on extraction processes (liquid-liquid, solid-phase and related techniques) applied to the biomatrix. In the review, chiral, isotope-labeling, hydrophobicity-tailored and post-column derivatizations are also included, based on representative publications in the literature during the last two decades. Examples of derivatization reagents and brief reaction conditions are included, together with some bioanalytical applications and performances (chromatographic conditions, detection limit, stability and sample biomatrix).

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Derivatization procedures and their analytical performances for HPLC determination in bioanalysis

David

Moldoveanu

Galaon

2020

Biomedical Chromatography

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Biomarker ratios

Cawley

Keen

Tou

et al. 2022

Drug Testing and Analysis

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Simple, selective, and identifiable analysis of aromatic monoamines with a surrogate on sulfuric acid impregnated filters by derivatization with an acid chloride reagent and HPLC with fluorescence detection

Inoue

2018

J of Separation Science

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A simple and selective derivatization and detection method for o‐toluidine, o‐anisidine, and 2,4‐dimethylaniline was developed using a sulfuric acid impregnated filter, 4‐(N‐chloroformylmethyl‐N‐methylamino)‐7‐nitro‐2,1,3‐benzoxadiazole, and a surrogate (o‐ethylaniline), which allowed simple, rapid, selective, and identifiable analysis by high‐performance liquid chromatography with fluorescence detection. These amines were successfully derivatized in a pH 5 buffer solution containing acetonitrile at 35°C for 10 min (linear in the range 0–400 ng/mL, n = 5, aromatic amines: r > 0.9980, aromatic amines with surrogate correction: r > 0.9997, aromatic amines in the presence of phenol and aliphatic amines with surrogate correction: r > 0.9996). The retention times of these derivatized aromatic amines were <17 min using isocratic elution. No derivatives corresponding to phenol or the aliphatic amines (i.e., cyclohexylamine and hexylamine) were detected in the reaction solution. This method can therefore be considered applicable for the analysis of samples from occupational environments, as samples were successfully extracted, derivatized, and measured by high‐performance liquid chromatography following spiking of a sulfuric acid impregnated filter with aromatic amines, phenols, and aliphatic amines. Indeed, only the aromatic amines were quantitatively detected without pretreatment, with no other spiked compounds being detected.

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Derivatization-based High-throughput Bioanalysis by LC-MS

Cited by 41 publications

References 90 publications

Derivatization procedures and their analytical performances for HPLC determination in bioanalysis

Derivatization procedures and their analytical performances for HPLC determination in bioanalysis

Biomarker ratios

Simple, selective, and identifiable analysis of aromatic monoamines with a surrogate on sulfuric acid impregnated filters by derivatization with an acid chloride reagent and HPLC with fluorescence detection

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