Derivative Spectrophotometric Determination of Levodopa and Benserazide Hydrochloride

Development of a sensitive flow‐injection–chemiluminescence detection method for the determination of laevodopa

“…When KMnO 4 concentration was increased from 1.0 × 10 −4 to 0.5 × 10 -3 mol/L, the CL signal increased slowly. This might be attributed to subsequent destruction of the formed emitter by excess KMnO 4 or to the absorption of the emission by excess KMnO 4 . Therefore, 0.5 × 10 -3 mol/L KMnO 4 was chosen for subsequent experiments.…”

“…Several techniques have been reported in the literature for the determination of laevodopa, such as spectrofluorimetry (3), spectrophotometry (3)(4)(5)(6)(7)(8), ion-selective electrode (9), NMR spectroscopy (10), flow-injection analysis (FIA) (11)(12)(13)(14), and high-performance liquid chromatography (HPLC) (15)(16)(17). To our knowledge, there have been a few reports for the determination of laevodopa based on a chemiluminescence (CL) analysis system.…”

“…The present study investigated the effect of laevodopa on the CL intensity emitted from the reaction of sulphite with acidic KMnO 4 . A sensitizing effect of laevodopa on CL emission was observed.…”

Simultaneous determination of levodopa and benserazide by stopped-flow injection analysis and three-way multivariate calibration of kinetic-spectrophotometric data

“…It is used in the treatment of Parkinson's disease, usually associated with a peripheral aromatic-l-amino acid decarboxylase (AADC) inhibitor, such as benserazide [N -(2,3,4-trihydroxybenzyl)-d,l-serine], in order to increment the proportion that enters the brain [1,2]. Scientific literature reports several methods for the determination of levodopa and benserazide in pharmaceutical preparations and in biological fluids, such as spectrophotometry [3][4][5][6][7][8][9], capillary zone electrophoresis [10,11], high performance liquid chromatography [12][13][14][15] and gas chromatography [16]. Flow injection analysis (FIA) has been applied to the determination of levodopa in mixtures with other drugs [17] and of benserazide in tablets [18].…”

Simultaneous Chemiluminometric Determination of Levodopa and Benserazide in a Multi-pumping Flow System with Multivariate Calibration

“…Due to the chemical similarity of the two compounds, simultaneous determination was achieved using differential spectrophotometry, 2 derivative spectrophotometry [3][4][5] and kineticspectrophotometric methods. 6,7 Capillary electrophoresis (CE) with spectrophotometric 8,9 and amperometric detection 10 was also used for simultaneous determination of these drugs.…”