Derivation of Putative Porcine Embryonic Germ Cells and Analysis of Their Multi-Lineage Differentiation Potential

Cong, Yimei; Ma, Jing; Sun, Ruizhen; Wang, Yunlong; Xue, Binghua; Wang, Jiaqiang; Xie, Bingteng; Wang, Juan; Hu, Kui; Liu, Zhong Hua

doi:10.1016/j.jgg.2013.06.003

Cited by 10 publications

(8 citation statements)

References 37 publications

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“…In aligned nanofibers, we observed higher Nestin expression during spontaneous compared with inductive differentiation, because Nestin is also expressed in non‐neural stem cell populations, such as pancreatic islet and hematopoietic progenitors. Further, EBs form mesendodermal derivatives, including skeletal myocytes, endothelial cells, hepatocytes, cardiomyocytes, and pancreatic cells . These results indicate that EBs have the potential for multilineage differentiation during spontaneous differentiation.…”

Section: Discussionmentioning

confidence: 73%

“…Further, EBs form mesendodermal derivatives, including skeletal myocytes, endothelial cells, hepatocytes, cardiomyocytes, and pancreatic cells. 15 These results indicate that EBs have the potential for multilineage differentiation during spontaneous differentiation.…”

Section: Discussionmentioning

confidence: 81%

“…Es cell–Es cell contact and consequent intercellular signaling is minimized in a monolayer culture. EB formation allows the formation of cell clusters and complex interactions between heterogeneous cells that results in the differentiation of cells to all three embryonic germ layers: endodermal, mesodermal, and ectodermal derivatives . The exposure of ectodermal cells to RA increases neural induction.…”

Section: Discussionmentioning

confidence: 99%

“…EB formation allows the formation of cell clusters and complex interactions between heterogeneous cells that results in the differentiation of cells to all three embryonic germ layers: endodermal, mesodermal, and ectodermal derivatives. 15 The exposure of ectodermal cells to RA increases neural induction. Our study confirmed that the addition of 1 lM RA results in a high proportion of cells becoming neurons, oligodendrocytes, and astrocytes, whereas aggregation alone without a growth factor directs the cells to non-neural cell types, including fibroblasts, adipocytes and pulsating cardiac cells.…”

Section: Discussionmentioning

confidence: 99%

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Influence of oriented nanofibrous PCL scaffolds on quantitative gene expression during neural differentiation of mouse embryonic stem cells

Abbasi

Hashemi

Salehi³

et al. 2015

J Biomedical Materials Res

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Neural differentiation of mouse embryonic stem cells in combination with three-dimensional electrospun nanofibers as an artificial extracellular matrix can be utilized to reconstruct a spinal cord defect. In this study, random and parallel-aligned nanofibrous poly ɛ-caprolactone was fabricated using electrospinning. Its hydrophobicity was modified by O2 plasma treatment to facilitate enhanced cell attachment. Embryoid bodies (EBs), which contain all three embryonic germ layers, were cultured on poly ɛ-caprolactone scaffolds to study the effect of fiber orientation on cell morphology and differentiation. Cell morphology and neuron-specific gene and protein expressions were, respectively, evaluated by scanning electron microscopy, real-time polymerase chain reaction, and immunocytochemistry. Although two types of nanofibrous scaffolds showed neural marker expression at the protein level, cells on randomly oriented scaffolds showed short-range topographical guidance and stretched across multiple directions, whereas cells on the parallel scaffolds exhibited long extension with enhanced neuron outgrowth along the fiber, producing oriented extracellular matrix, leading to direct cell migration and nerve regeneration. Quantitative real-time polymerase chain reaction showed that both aligned and random electrospun nanofibers downregulated the precursor neural marker Nestin compared with that in the control group, a gelatin-coated tissue culture plate (T). Analysis also showed higher expression of dorso-ventral neural markers (Isl1/2 and Lim1/2) than motor neuron progenitor markers (Pax6, Nkx6.1, and olig2) in aligned nanofibers than in the T group. Moreover, aligned nanofibers showed higher expression of mature neural specific markers such as β-tub and Map2 than those in the randomly oriented scaffolds. Therefore, we conclude that nanofibers with different orientations can support the neural lineage, but aligned nanofibrous scaffolds are superior candidates to promote the advancement of neural precursors to achieve maturity during the differentiation process.

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Section: Discussionmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Influence of oriented nanofibrous PCL scaffolds on quantitative gene expression during neural differentiation of mouse embryonic stem cells

Abbasi

Hashemi

Salehi³

et al. 2015

J Biomedical Materials Res

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“…O modelo suíno possibilita a realização de modificações gênicas complexas e precisas com intuito de estudar a terapia celular aplicada em animal de porte maior visando benefícios à biomedicina e agropecuária (WEST et al, 2010). A fisiologia, morfologia e sistema imune semelhante ao do humano, inclusive no tamanho dos órgãos permitindo a extrapolação dos dados adquiridos, torna-o um modelo mais interessante biologicamente (EZASHI et al, 2009;WU et al, 2009;WEST et al, 2010;CONG et al, 2013;ROBERTS, 2016;KIM et al, 2019). Além de ser um animal comum na produção animal tendo sua criação e manuseio já rotineiros e padronizados quando comparada a outros modelos animais, atualmente, já é utilizado na biomedicina.…”

Section: Introductionunclassified

>b<Indução da pluripotência celular e diferenciação >i<in vitro>/i< no modelo suíno como modelo translacional>/b<

Machado¹

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Me faltam palavras para descrever como foi esta jornada chamada mestrado. Assim como me faltam palavras para agradecer todos aqueles que tornaram tudo isso uma realidade. O primeiro agradecimento precisa ser para Fabiana Bressan. Muito mais do que uma orientadora e professora. Há mais de 4 anos, junto com Paulo Fantinato, me incentivando a ser ambicioso e buscar mais. Confiando em mim, mais do que eu mesmo. Me oferecendo oportunidades que muitos sonhariam em ter. Nunca me deixando desanimar, e sempre pronta para me ajudar e ensinar. Além dos ocasionais "puxões de orelha" quando necessário. Até por eles sou grato (mas não tanto quanto o resto). À ambos, meu muito obrigado. Aos meus companheiros, colegas e amigos do "grupo iPS". Do começo ao fim juntos, nas partes boas e nas partes ruins (ou como diriam alguns, "dias de luta, dias de glória"). À minha companheira de aventuras em terras nórdicas, pelos muitos conselhos (mesmo que eu não concorde sempre) e sempre por tentar fazer eu sair da minha caixinha, Naira Pieri. Ao meu companheiro de conversas mais diversas possíveis, inclusive polêmicas, e piadas mais inusitadas, Ramon Botigelli. À minha companheira de cultivo celular noturnos e discussões filosóficas sobre comunicação com as células, dentre outros temas, Raquel Castro. À minha grande incentivadora de tentar coisas novas, na ciência e na vida, Aline Souza. À minha grande amiga que me acompanha desde os meus primeiros anos no laboratório com iniciação cientifica até o mestrado, Laís Pessôa. A todos vocês, meu muito obrigado. À minha companheira de aventuras em terras canadenses. Irmã em Cristo, companheira nas dúvidas científicas, teológicas e sobre todas as outras coisas da vida, Marina Amaro. Que juntamente com Alessandra Bridi, formam minhas companheiras de almoços longos e, os mais frequentes, almoços corridos. Amigas que a graduação e o mestrado me deram e fizeram os dias ruins, bons, e os bons, melhores. Meu muito obrigado.

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Embryonic Stem Cells and Fetal Development Models

Nowak‐Imialek¹,

Niemann²

2016

Fetal Stem Cells in Regenerative Medicine

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Derivation of Putative Porcine Embryonic Germ Cells and Analysis of Their Multi-Lineage Differentiation Potential

Cited by 10 publications

References 37 publications

Influence of oriented nanofibrous PCL scaffolds on quantitative gene expression during neural differentiation of mouse embryonic stem cells

Influence of oriented nanofibrous PCL scaffolds on quantitative gene expression during neural differentiation of mouse embryonic stem cells

>b<Indução da pluripotência celular e diferenciação >i<in vitro>/i< no modelo suíno como modelo translacional>/b<

Embryonic Stem Cells and Fetal Development Models

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