Derivation of multipotent nestin+/CD271−/STRO-1− mesenchymal-like precursors from human embryonic stem cells in chemically defined conditions

Wu, Rongrong; Gu, Bin; Zhao, Xiaoli; Tan, Zhou; Chen, Liangbiao; Zhu, Jiang; Zhang, Ming

doi:10.1007/s13577-011-0022-3

Cited by 26 publications

(18 citation statements)

References 35 publications

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“…We have not included nestin, Sox2, or Oct4, in the analysis, since these are intracellular proteins but not surface markers. However, we noticed that nestin(+)/CD271(−)/Stro‐1(−) MSCs derived from human ESCs were reported to differentiate into representative derivatives of all three embryonic germ layers . Therefore, compared with CD271 and Stro‐1, nestin positivity may represent a more primitive phenotype of MSCs.…”

Section: Discussionmentioning

confidence: 99%

Concise Review: The Surface Markers and Identity of Human Mesenchymal Stem Cells

et al. 2014

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The concept of mesenchymal stem cells (MSCs) is becoming increasingly obscure due to the recent findings of heterogeneous populations with different levels of stemness within MSCs isolated by traditional plastic adherence. MSCs were originally identified in bone marrow and later detected in many other tissues. Currently, no cloning based on single surface marker is capable of isolating cells that satisfy the minimal criteria of MSCs from various tissue environments. Markers that associate with the stemness of MSCs await to be elucidated. A number of candidate MSC surface markers or markers possibly related to their stemness have been brought forward so far, including Stro-1, SSEA-4, CD271, and CD146, yet there is a large difference in their expression in various sources of MSCs. The exact identity of MSCs in vivo is not yet clear, although reports have suggested they may have a fibroblastic or pericytic origin. In this review, we revisit the reported expression of surface molecules in MSCs from various sources, aiming to assess their potential as MSC markers and define the critical panel for future investigation. We also discuss the relationship of MSCs to fibroblasts and pericytes in an attempt to shed light on their identity in vivo.

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Section: Discussionmentioning

confidence: 99%

Concise Review: The Surface Markers and Identity of Human Mesenchymal Stem Cells

et al. 2014

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“…Increased expression of Klf 4 and c myc, ESC specific transcription fac tors, has also been found . The expres sion of undifferentiated ESC markers in MSCs proba bly explains their higher differentiation potential (Wu et al, 2013). Enhanced expression of these markers in spheroids compared to monolayer cultures suggests that they are primitive subpopulations with multipo tent potential (Bogdanova Jatniece et al, 2014;Guo et al, 2014).…”

Section: Surface Marker Expression In Fetmsc and Fetmsc And Their Sphmentioning

confidence: 99%

Cellular spheroids obtained from mesenchymal stem cells derived from bone marrow and limb muscle of early human embryo

et al. 2015

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Cellular spheroids were obtained from mesenchymal stem cells derived from different tissues of 5 to 6 week old embryos: bone marrow (FetMSC) and limb muscle (M FetMSC). The properties of these cells maintained in monolayer (2D cultivation) or spheroids (3D cultivation) were compared. Cellular sphe roids were obtained from monolayer cultures at the sixth passage after decryopreservation and assayed 48 h after their formation. Unlike monolayer cultures, spheroids are heterogeneous cell populations consisted of fibroblast like and epithelioid cells. Two day old spheroids are actively proliferating structures. Cell surface markers were analyzed by flow cytometry. Monolayer culture and cellular spheroids expressed CD44, CD73, CD90, CD105, and HLA ABC common for mesenchymal stem cells (MSCs) and lacked CD34 and HLA DR. However, expression of CD90 and CD105 antigens was significantly lower in spheroids than in mono layer cultures. Expression of transcription factors and surface antigens typical for human embryonic stem cells were analyzed with immunofluorescence and flow cytometry. Expression of Sox 2 and SSEA 4 in 2D and 3D cultures, lack of Oct 4 in 2D cultures, and its increase in 3D cultures were found. Both monolayer cells and spheroids were able to differentiate into osteogenic, chondrogenic, and adipogenic lineages, although monolayer cultures and spheroids differed. Adipogenic differentiation was more active in the cellular spheroids from M FetMSC cells compared to corresponding monolayer cultures. Differences between the 2D and 3D cultures of both lines have been shown by the character of chondrogenic differentiation. As a whole, these findings confirm the MSC status for the cellular spheroids derived from FetMSC and M FetMSC mono layer cultures and attest to their increased differentiation potential compared to monolayer cultures.Abbreviations: ECM-extracellular matrix, MSC-mesenchy mal stem cell, ESC-embryonic stem cell, 2D-two dimen sional culture system, 3D-three dimensional culture system. 432 CELL AND TISSUE BIOLOGY Vol. 9No. 6 2015 KRYLOVA et al.

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“…This strategy is supported by step-wise protocols to achieve MSC differentiation and then chondrogenesis. To date, several reports have described the derivation of MSCs from hESCs for subsequent chondrogenic differentiation and cartilage tissue engineering (99–102). The implanted hESC-derived mesenchymal cells gave rise to homogeneous, well-differentiated tissues exclusively of mesenchymal origin and without teratoma formation (103).…”

Section: Stem Cells For Cartilage Tissue Engineeringmentioning

confidence: 99%

Time-Dependent Processes in Stem Cell-Based Tissue Engineering of Articular Cartilage

Gadjanski

Spiller

Vunjak-Novakovic

2011

Stem Cell Rev and Rep

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Articular cartilage (AC), situated in diarthrodial joints at the end of the long bones, is composed of a single cell type (chondrocytes) embedded in dense extracellular matrix comprised of collagens and proteoglycans. AC is avascular and alymphatic and is not innervated. At first glance, such a seemingly simple tissue appears to be an easy target for the rapidly developing field of tissue engineering. However, cartilage engineering has proven to be very challenging. We focus on time-dependent processes associated with the development of native cartilage starting from stem cells, and the modalities for utilizing these processes for tissue engineering of articular cartilage.

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Derivation of multipotent nestin+/CD271−/STRO-1− mesenchymal-like precursors from human embryonic stem cells in chemically defined conditions

Cited by 26 publications

References 35 publications

Concise Review: The Surface Markers and Identity of Human Mesenchymal Stem Cells

Concise Review: The Surface Markers and Identity of Human Mesenchymal Stem Cells

Cellular spheroids obtained from mesenchymal stem cells derived from bone marrow and limb muscle of early human embryo

Time-Dependent Processes in Stem Cell-Based Tissue Engineering of Articular Cartilage

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