Derivation of HVR1, HVR2 and HVR3 human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis (PGD) for monogenic disorder

Hmadcha, Abdelkrim; Aguilera, Yolanda; Lozano-Arana, Maria Dolores; Mellado, Nuria; Sánchez, Javier; Moya, Cristina Fortuno; Sánchez-Palazón, Luis; Palacios, J.M.; Antiñolo, Guillermo; Soria, Bernat

doi:10.1016/j.scr.2016.03.012

Cited by 1 publication

(1 citation statement)

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“…The UN-hESC HS181 line was maintained and passaged as previously described. 65 , 66 The protocol of hESC directed differentiation into insulin-producing beta-like cells (DD-hESCs) and the protocol of spontaneous differentiation (SD-hESCs) were achieved, as described by Pezzolla et al. 36 Cells generated at the final stage were then transfected with miR-7 mimic (10–100 nM) and cultured in differentiation medium to facilitate the formation of cell clusters and to mature the beta cell-like phenotype.…”

Section: Methodsmentioning

confidence: 99%

miR-7 Modulates hESC Differentiation into Insulin-Producing Beta-like Cells and Contributes to Cell Maturation

López‐Beas

Capilla-González

Aguilera

et al. 2018

Molecular Therapy - Nucleic Acids

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Human pluripotent stem cells retain the extraordinary capacity to differentiate into pancreatic beta cells. For this particular lineage, more effort is still required to stress the importance of developing an efficient, reproducible, easy, and cost-effective differentiation protocol to obtain more mature, homogeneous, and functional insulin-secreting cells. In addition, microRNAs (miRNAs) have emerged as a class of small non-coding RNAs that regulate many cellular processes, including pancreatic differentiation. Some miRNAs are known to be preferentially expressed in islets. Of note, miR-375 and miR-7 are two of the most abundant pancreatic miRNAs, and they are necessary for proper pancreatic islet development. Here we provide new insight into specific miRNAs involved in pancreatic differentiation. We found that miR-7 is differentially expressed during the differentiation of human embryonic stem cells (hESCs) into a beta cell-like phenotype and that its modulation plays an important role in generating mature pancreatic beta cells. This strategy may be exploited to optimize the potential for in vitro differentiation of hESCs into insulin-producing beta-like cells for use in preclinical studies and future clinical applications as well as the prospective uses of miRNAs to improve this process.

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Section: Methodsmentioning

confidence: 99%