Derivation of glycine from threonine in Escherichia coli K-12 mutants

Fraser, J.; Newman, E. B.

doi:10.1128/jb.122.3.810-817.1975

Cited by 47 publications

(25 citation statements)

References 17 publications

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“…This pathway could also lead to in creasedf'l' (a-Gly} values. However, it is activated only for certain E. coli strains in the presence of exogeneously supplied Thr (Fraser and Newman, 1975). Consistently, no increase of ffl) {a-Gly} is observed in the aerobic regime.…”

Section: Glycolysismentioning

confidence: 60%

Biosynthetically Directed Fractional 13C-labeling of Proteinogenic Amino Acids. An Efficient Analytical Tool to Investigate Intermediary Metabolism

Szyperski¹

1995

Eur J Biochem

155

331

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Section: Glycolysismentioning

confidence: 60%

Biosynthetically Directed Fractional 13C-labeling of Proteinogenic Amino Acids. An Efficient Analytical Tool to Investigate Intermediary Metabolism

Szyperski¹

1995

Eur J Biochem

155

331

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“…We propose here that the known inhibition of GlyA by glycine combined with the postulated inhibition of reaction 5 resulting from the unusually high serine concentration in the triple mutant, and the other amino acids in casamino acids, results in starvation for C1-THF and SAM. (Fraser and Newman, 1975). These enzyme reactions are regulated so as to permit either serine or glycine to be the precursor of the other.…”

Section: A Tentative Biochemical Explanation As To How L-sd Deficiencmentioning

confidence: 99%

Deficiency in l‐serine deaminase results in abnormal growth and cell division of Escherichia coli K‐12

Zhang

Newman

2008

Molecular Microbiology

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SummaryThe loss of the ability to deaminate L-serine severely impairs growth and cell division in Escherichia coli K-12. A strain from which the three genes (sdaA, sdaB, tdcG) coding for this organism's three L-serine deaminases had been deleted grows well in glucose minimal medium but, on subculture into minimal medium with glucose and casamino acids, it makes very large, abnormally shaped cells, many of which lyse. When inoculated into Luria-Bertani (LB) broth with or without glucose, it makes very long filaments. Provision of S-adenosylmethionine restores cell division in LB broth with glucose, and repairs much of the difficulty in growth in medium with casamino acids. We suggest that replication of E. coli is regulated by methylation, that an unusually high intracellular L-serine concentration, in the presence of other amino acids, starves the cell for Sadenosylmethionine and that it is the absence of S-adenosylmethionine and/or of C1-tetrahydrofolate derivatives that prevents normal cell division.

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“…Furthermore it has been suggested that intracellular leucine might serve as a signal for nitrogen-scavenging during periods of amino acid imbalances. 29 In the present work fermentation-utilisation patterns of D-frUCtOtX of the five growth-stimulatory amino acids (L-glutamine, L-arginine, L-histidine, L-methionine, L-proline), and of the precursor L-phenylalanine, were followed under varying pH and aeration rates. In previous studies by Matteo et ~1 .…”

Section: Discussionmentioning

confidence: 97%

Fermentation patterns of gramicidin S formation by Bacillus brevis ATCC 9999 in high‐yielding synthetic medium

Vandamme

Leyman

1981

J. Chem. Technol. Biotechnol.

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Addition of the gramicidin S (GS)‐constituent amino acids, other than the limiting precursor L‐phenylalanine, to the high‐yielding chemically defined F3/6 and G3/6 media, enhanced growth and volumetric GS‐production by Bacillus brevis ATCC 9999 considerably, but did not yield a higher specific GS‐production level. L‐Leucine alone could duplicate this stimulatory effect in G3/6 medium. Replacing the fructose component of F3/6 medium by these four amino acids yielded a high specific GS‐production level, but resulted in poor growth and low volumetric antibiotic production. Nutrient‐utilisation patterns in F3/6 medium revealed that B. brevis initially grew at the expense of L‐glutamine and L‐arginine. After a diauxic lag period, D‐fructose was consumed together with L‐histidine. L‐Proline was mainly used during the stationary phase. L‐Methionine was broken down gradually throughout the whole fermentation cycle. L‐Phenylalanine was metabolised only after GS formation started, and its disappearance was proportional to the amount of GS produced. Lowering the aeration rate caused an acidification of the medium, resulting in a slower and incomplete, although similar, nutrient‐utilisation pattern. At a controlled pH of 7.3, under lowered aeration, utilisation patterns were again comparable with those of a fully aerated fermentation, but GS levels were enhanced considerably (0.220 mg of GS mg−1 dry cell wt). Depending on environmental culture conditions, B. brevis also excreted different amino acids (L‐lysine, L‐alanine, L‐valine, L‐serine), which were in turn metabolised during late growth and differentiation stages. The onset of GS synthesis occurred on depletion of L‐glutamine and L‐arginine. Soluble GS synthetase 1 and 2 peaks coincided with ‘diauxic’ lag phases; this supported the idea that a high growth rate is incompatible with GS synthetase formation.

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Derivation of glycine from threonine in Escherichia coli K-12 mutants

Cited by 47 publications

References 17 publications

Biosynthetically Directed Fractional 13C-labeling of Proteinogenic Amino Acids. An Efficient Analytical Tool to Investigate Intermediary Metabolism

Biosynthetically Directed Fractional 13C-labeling of Proteinogenic Amino Acids. An Efficient Analytical Tool to Investigate Intermediary Metabolism

Deficiency in l‐serine deaminase results in abnormal growth and cell division of Escherichia coli K‐12

Fermentation patterns of gramicidin S formation by Bacillus brevis ATCC 9999 in high‐yielding synthetic medium

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