Objective
To evaluate the potential of photodynamic therapy (PDT) with blue lightâemitting diode (LED) 460ânm at 25, 50 and 100âJ/cm2 using three concentrations of acai extracts (100, 40, and 10Â mg/ml), in the proliferation and viability of head and neck tumor lines (SCC9).
Methods
Three groups of cells were analyzed for 3âdays in an in vitro assay with MTT (3â (4,5âdimethylthiazolâ2âyl) â2,5, âdiphenyltetrazolium bromide) and crystal violet: cells in the absence of acai extract and PDT (control group); cells in the presence of acai extract and no light; and cells in the presence of acai extract and LED blue light (PDT groups).
Results
When using acai as a PS combined with blue LED (460ânm, 0.7466âcm2, 1000âmW/cm2) and irradiation at 25, 50, and 100âJ/cm2, after 72âh, cell viability (pâ<â0.0001 vs. control, p = 0.0027 vs. 100âmg/ml açai group, p = 0.0039 vs. 40âmg/ml açai group, p = 0.0135 vs. 10âmg/ml açai group; OneâWay ANOVA/Tukey) and proliferation (pâ<â0.05, OneâWay ANOVA/Tukey) decreased.
Conclusion
The acai in question is a potential photosensitizer (PS), with blue light absorbance and efficacy against head and neck tumor lines (SCC9).