Deregulation of long noncoding RNAs ANCR, TINCR, HOTTIP and SPRY4-IT1 in plasma of systemic sclerosis patients: SPRY4-IT1 as a novel biomarker of scleroderma and its subtypes

Systemic Sclerosis Patients With Negative Antinuclear Antibodies Have Distinctive Clinical Manifestations: a Multicenter CRDC Cohort in China

“…We detected anti-Scl-70 positivity in 46.4% of SSc patients (37.5% in lcSSc, 54.9% in dcSSc), along with anti-ACA positivity of 20.0% (23.1% in lcSSc, 15.5% in dcSSc); anti-RNP III antibody was found in 10.0% of SSc patients. Positive rates of those SSc-speci c autoantibodies vary among different study populations, suggesting racial divergence [14]. Moreover, encouragingly supported by our study and previous one, autoantibodies tend to be speci c for clinical characteristics.…”

Circulating long non-coding RNAs HOTAIR, Linc-p21, GAS5 and XIST expression profiles in diffuse large B-cell lymphoma: association with R-CHOP responsiveness

“…We used GAPDH as the endogenous control to normalize lncRNAs. GAPDH was reported to be stably expressed in plasma and was previously selected as an internal control in plasma to normalize lncRNAs 56,57 . In www.nature.com/scientificreports/ addition, GAPDH level was not affected by age, sex and pathology in human plasma 57 , and was regarded as an ideal internal control for plasma assays 56,57 .…”

“…GAPDH was reported to be stably expressed in plasma and was previously selected as an internal control in plasma to normalize lncRNAs 56,57 . In www.nature.com/scientificreports/ addition, GAPDH level was not affected by age, sex and pathology in human plasma 57 , and was regarded as an ideal internal control for plasma assays 56,57 . The primers sequences were as follows: 5′-GGT AGA AAA AGC AAC CAC GAAGC-3′ (forward) and 5′-ACA TAA A-CCT CTG TCT GTG AGT GCC -3′ (reverse) for HOTAIR; 5′-GGG TGG CTC ACT CTT CTG GC-3′ (forward) and 5′-TGG CCT TGC CCG GGC TTG TC-3′ (reverse) for Linc-p21; 5′-GTG TGG CTC TGG -ATA GCA C-3′ (forward) and 5′-ACC CAA GCA AGT CAT CCA TG-3′ (reverse) for GAS5; 5′-GCA TAA CTC GGC TTA GGG CT-3′ (forward) and 5′-TCC TCT GCC TGA CCT GCT AT-3′ (reverse) for XIST; 5′-CCC TTC ATT GAC CTC AAC TA-3′ (forward) and 5′-TGG AAG ATG GTG ATG GGA TT -3′ (reverse) for GAPDH.…”

Serum a proliferation-inducing ligand and MicroRNA-223 are associated with rheumatoid arthritis: diagnostic and prognostic implications

“…miRNAs can control many immune processes, including T- and B-cell development and maturation, antigen presentation, Toll-like receptor signaling and pro-inflammatory cytokine production, immunoglobulin class-switch recombination in B-cells, and T-cell receptor signaling (Ceribelli et al 2012 ). Differential expression of non-coding RNAs, including miRNAs were found in patients affected by several autoimmune diseases, and were linked to the pathogenesis of these conditions (Senousy et al 2019 ; Senousy et al 2020 ; Abd-Elmawla et al 2020 ). Indeed, dysregulated miRNA expression has been shown to be implicated into the molecular mechanisms of RA (Tavasolian et al 2018 ).…”