Deregulation of Gene Expression Induced by Environmental Tobacco Smoke Exposure in Pregnancy

Votavová, Hana; Merkerová, Michaela Dostálová; Krejčík, Zdeněk; Fejglova, Kamila; Vasikova, Alzbeta; Pastorková, Anna; Tabashidze, Nana; Topinka, Jan; Balaščák, Ivan; Srám, Radim J.; Brdička, R

doi:10.1093/ntr/ntr325

Cited by 35 publications

(26 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Votavova et al compared the transcriptome of the term placenta in smokers and non-smokers using a microarray analysis. Their findings demonstrated increased expression of genes related to coagulation and vasculogenesis and decreased expression of cell adhesion-related genes [19], [20]. In a recent study of the placental DNA methylation in term placentas, maternal smoking was found to deregulate the placental methylation in a CpG site-specific manner that correlated with meaningful alterations in gene expression among signature pathways [21].…”

Section: Introductionmentioning

confidence: 98%

Effects of Maternal Smoking on the Placental Expression of Genes Related to Angiogenesis and Apoptosis during the First Trimester

et al. 2014

View full text Add to dashboard Cite

ObjectiveMaternal cigarette smoking is reportedly associated with miscarriage, fetal growth restriction and placental abruption, and is paradoxically associated with a decreased risk of developing preeclampsia. In the present study, we investigated the gene expression levels of villous tissues in early gestation. We compared the expression levels of the genes related to angiogenesis and apoptosis in the villous tissues obtained from smoking and non-smoking pregnant women.Materials and MethodsWe collected villous tissue samples from 57 women requesting surgical termination due to non-medical reasons at 6–8 weeks of gestation. The maternal cigarette smoking status was evaluated by the level of serum cotinine and patients were divided into active smokers and non-smokers by the serum cotinine level. The placental levels of VEGFA, PGF, FLT1, HIF1A, TP53, BAX and BCL2 mRNA were quantified by real time PCR.ResultsThe gene expression level of PGF and HIF1A in the active smoker group was significantly higher than that in the non-smoker group. We did not observe any significant differences in the VEGFA or FLT1 expression between the groups. In active smoker group, the gene expression levels of TP53 and BAX were significantly higher than those in the non-smoker group. The ratio of BAX/BCL2 mRNA in the active smoker group was significantly higher than that in the non-smoker group.ConclusionsOur findings revealed that smoking might affect the placenta during early pregnancy. Maternal cigarette smoking in early pregnancy may be associated with villus hypoxia, which may influence angiogenesis and apoptosis.

show abstract

Section: Introductionmentioning

confidence: 98%

Effects of Maternal Smoking on the Placental Expression of Genes Related to Angiogenesis and Apoptosis during the First Trimester

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Recent studies profiling gene expression by microarray analysis have shown that smoking causes significant expression changes in maternal, placental and fetal tissues, particularly in those genes involved in xenobiotic metabolism, oxidative stress, inflammation and immunity [19,20]. To our knowledge, no previous studies have explored the influence of smoking on the levels of angiogenic factors and oxidative stress in the early stages of pregnancy.…”

Section: Introductionmentioning

confidence: 99%

Maternal Smoking and Placental Expression of a Panel of Genes Related to Angiogenesis and Oxidative Stress in Early Pregnancy

et al. 2014

View full text Add to dashboard Cite

Objective: Maternal cigarette smoking is paradoxically associated with a decreased risk of developing preeclampsia. Since preeclampsia is thought to be associated with altered mechanisms of angiogenesis and oxidative stress, we aim to investigate the influence of maternal smoking on the early placental expression of a panel of genes related to angiogenesis and oxidative stress. Material and Methods: We collected villous tissue samples at 6-7 and 10-11 weeks of gestation from 31 women requesting surgical termination. Placental expression of the following genes were quantified by real-time PCR: vascular endothelial growth factor A (VEGFA), fms-like tyrosine kinase (Flt-1), soluble endoglin (sEng), placental growth factor (PlGF), heme oxygenase-1 (HMOX-1) and superoxide dismutase (SOD). Maternal smoking status was assessed by levels of serum cotinine. Results: Placental expression of VEGFA was significantly higher in smoking women at 10-11 weeks of gestation compared with nonsmoking women at the same gestational age. There was no significant difference at 6-7 weeks of gestation. There was no variation in the expression of the other genes explored related to smoking status. Conclusions: Here we report that VEGFA placental expression was higher in smoking women at 10-11 weeks of gestation. Increased VEGFA expression in the early stages of pregnancy in smoking women might contribute to the decreased risk of developing preeclampsia.

show abstract

“…Possibly contributing to impaired lung function are data that suggest that maternal smoke exposure may alter Clara cell secretory protein (CCSP) expression in fetal lungs [134]. Indeed, evidence currently suggests that maternal smoke exposure (including SHS) during pregnancy leads to the deregulation of gene expression [135]. Confirmatory primate studies have shown that in utero nicotine exposure adversely affects overall lung development by decreasing lung size and volume, elastin, while increasing Type I and Type III collagen, alveolar volume, and airway wall areas [136,137,138,139].…”

Section: Health Outcomes and Comorbiditiesmentioning

confidence: 99%

Plausible Roles for RAGE in Conditions Exacerbated by Direct and Indirect (Secondhand) Smoke Exposure

Lewis

Hirschi

Arroyo

et al. 2017

IJMS

View full text Add to dashboard Cite

Approximately 1 billion people smoke worldwide, and the burden placed on society by primary and secondhand smokers is expected to increase. Smoking is the leading risk factor for myriad health complications stemming from diverse pathogenic programs. First-and second-hand cigarette smoke contains thousands of constituents, including several carcinogens and cytotoxic chemicals that orchestrate chronic inflammatory responses and destructive remodeling events. In the current review, we outline details related to compromised pulmonary and systemic conditions related to smoke exposure. Specifically, data are discussed relative to impaired lung physiology, cancer mechanisms, maternal-fetal complications, cardiometabolic, and joint disorders in the context of smoke exposure exacerbations. As a general unifying mechanism, the receptor for advanced glycation end-products (RAGE) and its signaling axis is increasingly considered central to smoke-related pathogenesis. RAGE is a multi-ligand cell surface receptor whose expression increases following cigarette smoke exposure. RAGE signaling participates in the underpinning of inflammatory mechanisms mediated by requisite cytokines, chemokines, and remodeling enzymes. Understanding the biological contributions of RAGE during cigarette smoke-induced inflammation may provide critically important insight into the pathology of lung disease and systemic complications that combine during the demise of those exposed.

show abstract

Deregulation of Gene Expression Induced by Environmental Tobacco Smoke Exposure in Pregnancy

Abstract: This study demonstrates that even low dose exposure to ETS during pregnancy leads to significant deregulation of transcription in placental and fetal cells. These data suggest that the effect of ETS on the fetus is primarily indirect, mediated via deregulation of placental functions.

Cited by 35 publications

References 35 publications

Effects of Maternal Smoking on the Placental Expression of Genes Related to Angiogenesis and Apoptosis during the First Trimester

Effects of Maternal Smoking on the Placental Expression of Genes Related to Angiogenesis and Apoptosis during the First Trimester

Maternal Smoking and Placental Expression of a Panel of Genes Related to Angiogenesis and Oxidative Stress in Early Pregnancy

Plausible Roles for RAGE in Conditions Exacerbated by Direct and Indirect (Secondhand) Smoke Exposure

Contact Info

Product

Resources

About