Der p 2 isoallergens have different allergenicity, and quantification with 2‐site ELISA using monoclonal antibodies is influenced by the isoallergens

Abstract

We conclude that isoallergens of Der p 2 may have different IgE immune responses. Quantification of Der p 2 with 2-site ELISA kits that adopted mAbs, might be affected by the prevalent form of the isoallergens in reservoir dust.

Isoallergen Variations Contribute to the Overall Complexity of Effector Cell Degranulation: Effect Mediated through Differentiated IgE Affinity

“…In agreement with previous findings (14), our results have the practical implication that such Ab-based assays should preferably be validated regarding the ability of the mAbs to recognize all present isoallergens. Ideally, the mAbs should recognize an epitope fully conserved among all of the isoallergens to assure equally high binding affinity for correct measurements of the entire isoallergen repertoire present in the particular extract.…”

“…The ability to distinguish between a limited number of different Der p 2 isoallergens has been shown for IgG mAbs produced in mice (9,10,12) and for human IgE Abs at the level of polyclonal patient sera in ELISA (13,14). Still, the biological mechanism at the level of effector cell activation triggered by interactions between individual IgE Abs and Der p 2 isoallergens is largely unknown.…”

Allergic Rhinitis and its Impact on Asthma (ARIA) 2008*

“…A single recombinant allergen or a combination of a few major recombinant allergens can substitute the crude extract for in vitro diagnostic purposes (1258,1259). Another possibility is to add some relevant recombinant allergens to an allergen extract.…”

Mass spectrometry and pollen allergies

“…Different isoforms of pollen allergens often vary in their immune reactivity, as has been shown for the Bet v 1 allergens in birch pollen [22], for other respiratory allergens [23] and for food allergens [24]. In those cases in which variation exists in allergenicity of different isoforms, the allergenicity of pollen from a particular biological source is not determined by the total allergen content alone, but also by the quantities of the different isoforms and their allergenic potential.…”

“…The existence of multiple isoforms with varying IgE reactivity poses a challenge to this end. The observation that a high prevalence of isoforms is linked to a high IgE immune response [23] suggests that MS-based methods may speed up the selection of the candidate recombinant allergens. For example, ana lysis of the quantity of individual Bet v 1 isoforms in birch pollen [19] would have resulted in the selection of Bet v 1a as the most promising candidate for diagnosis, which is confirmed by testing on birch-allergic patients [34].…”