Der modifizierte Heidelberg-Retina-Tomograph HRT Erste Ergebnisse einer In-vivo-Darstellung von kornealen Strukturen

Stave, J.; Zinser, G.; Grümmer, G.; Guthoff, R.

doi:10.1007/s003470100535

Cited by 134 publications

(31 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…22 This microscope uses a 670-nm laser source, a class 1 laser system, and thus, by definition, does not pose any ocular safety hazard. The eye to be examined was first anesthetized by instilling proparacain 0.5% eye drops (Ursapharm, Saarbrü cken, Germany).…”

Section: In Vivo Confocal Laser-scanning Microscopymentioning

confidence: 99%

Corneal sensitivity and morphology of the corneal subbasal nerve plexus in primary congenital glaucoma

Gatzioufas

Labiris

Hafezi

et al. 2014

Eye

View full text Add to dashboard Cite

Background To quantify the corneal subbasal nerve density and the total number of nerve fibers in primary congenital glaucoma (PCG) and to evaluate their impact on corneal sensitivity. Methods Forty eyes of 26 PCG patients were compared with 40 eyes randomly selected from 40 non-glaucoma patients who populated the control group. Central corneal sensitivity (CCS) was assessed by means of Cochet-Bonnet esthesiometry. The mean subbasal nerve density and the total number of nerve fibers were quantified by laserscanning confocal microscopy. Normality of data was assessed by Kolmogorov-Smirnov testing. Differences in parameters were assessed with Student's t-test, while correlations with CSS were assessed with Pearson's correlation. Results Significant differences were identified in the mean subbasal nerve density (2108 ± 692 mm in PCG, 2642 ± 484 mm in controls, P ¼ 0.003) and in the total number of nerve fibers (12.3±4.2 in PCG, 15.4±3.1 in controls, P ¼ 0.02). Both groups presented comparable mean CCS and tortuosity. Both groups presented strong correlations between CCS and mean nerve density (r ¼ 0.57 in PCG, r ¼ 0.67 in controls, all Po0.05), and between CCS and total number of nerve fibers (r ¼ 0.55 in PCG, r ¼ 0.56 in controls, all Po0.05). Conclusion PCG exerts significant changes in both the mean subbasal nerve density and the total number of nerve fibers. However, these changes do not appear to affect central corneal sensitivity.

show abstract

Section: In Vivo Confocal Laser-scanning Microscopymentioning

confidence: 99%

Corneal sensitivity and morphology of the corneal subbasal nerve plexus in primary congenital glaucoma

Gatzioufas

Labiris

Hafezi

et al. 2014

Eye

View full text Add to dashboard Cite

show abstract

“…Contactless operation together with the use of an NIR LED made the examination procedure comfortable for the subjects. All the images had a large FOV of 1.26 X 1.26 mm 2 . We obtained images of epithelium, Bowman's layer, sub-basal nerve plexus (SNP), anterior, middle and posterior stroma, endothelial cells with visible nuclei.…”

Section: Resultsmentioning

confidence: 99%

“…Currently, confocal microscope, given its ability of en face viewing with a micrometer-level resolution 2,3 , is the only clinical tool capable to see these features through the entire corneal depth. Unfortunately, confocal microscopy is not exempt from drawbacks, being the relatively small field-of-view and typical requirement of contact with an eye.…”

Section: Introductionmentioning

confidence: 99%

In vivo imaging through the entire thickness of human cornea by full-field optical coherence tomography

Mazlin

Xiao

Dalimier

et al. 2018

Ophthalmic Technologies XXVIII

View full text Add to dashboard Cite

Despite obvious improvements in visualization of the in vivo cornea through the faster imaging speeds and higher axial resolutions, cellular imaging stays unresolvable task for OCT, as en face viewing with a high lateral resolution is required. The latter is possible with FFOCT, a method that relies on a camera, moderate numerical aperture (NA) objectives and an incoherent light source to provide en face images with a micrometer-level resolution. Recently, we for the first time demonstrated the ability of FFOCT to capture images from the in vivo human cornea 1 . In the current paper we present an extensive study of appearance of healthy in vivo human corneas under FFOCT examination. En face corneal images with a micrometer-level resolution were obtained from the three healthy subjects. For each subject it was possible to acquire images through the entire corneal depth and visualize the epithelium structures, Bowman's layer, sub-basal nerve plexus (SNP) fibers, anterior, middle and posterior stroma, endothelial cells with nuclei. Dimensions and densities of the structures visible with FFOCT, are in agreement with those seen by other cornea imaging methods. Cellular-level details in the images obtained together with the relatively large field-of-view (FOV) and contactless way of imaging make this device a promising candidate for becoming a new tool in ophthalmological diagnostics.

show abstract

“…This module attaches to a commercial Heidelberg Engineering scanning laser ophthalmoscope that is typically used for retinal imaging [17]. The attached module shifts the focus of the laser beam from the retinal surface to the cornea and the focal position is operator controlled by the motion of an secondary movable lens.…”

Section: Confocal Microscopy Of the Eyementioning

confidence: 99%

Correlation of histology and linear and nonlinear microscopy of the living human cornea

Masters

2009

Journal of Biophotonics

View full text Add to dashboard Cite

Journal of BIOPHOTONICSThe morphology and the function of cellular and noncellular structures in the living human cornea can be determined with modern correlative linear and nonlinear optical microscopic techniques and histology. Correlative microscopy is based on the use of different optical techniques to study the same specimen, ideally at the same location within the specimen, in order to increase the functional and/or morphological understanding of the specimen. A case study to assess the effect of overnight lid-closure on in vivo human corneal morphology is presented to illustrate correlative linear microscopy and optical low-coherence reflectometry. Nonlinear multiphoton excitation microscopy provides functional information on cellular metabolism based on the intrinsic fluorescence from the reduced pyridine nucleotides and the oxidized flavoproteins. Second-harmonic generation microscopy, a scattering process that does not deposit net energy into the tissue, provides structural information on corneal collagen organization. Molecular thirdharmonic generation microscopy generates a signal in all materials and it an emerging technique. Coherent antiStokes Raman scattering microscopy provides chemical imaging for biology and medicine. The comparison and limitations of these microscopic modalities, linear and nonlinear microscopy applied to the cornea, and a review of some key findings is analyzed. A correlative integration and correlation of linear and nonlinear microscopies to study corneal function and structure is proposed to validate the clinical interpretation of microscopic images of the cornea.Confocal microscopy image of in vivo human corneal nerves in the anterior stroma *

show abstract

Der modifizierte Heidelberg-Retina-Tomograph HRT Erste Ergebnisse einer In-vivo-Darstellung von kornealen Strukturen

Cited by 134 publications

References 1 publication

Corneal sensitivity and morphology of the corneal subbasal nerve plexus in primary congenital glaucoma

Corneal sensitivity and morphology of the corneal subbasal nerve plexus in primary congenital glaucoma

In vivo imaging through the entire thickness of human cornea by full-field optical coherence tomography

Correlation of histology and linear and nonlinear microscopy of the living human cornea

Contact Info

Product

Resources

About