14C‐4‐cholesterol was injected into the invaginated yolks of newly hatched chicks and the distribution of the labelled molecules was recorded by autoradiography. 14C was homogeneously distributed in the yolks 1 h after the injection. After 4 h, traces of radioactivity were found in the liver and after 12 h 14C had been transported from the liver into most parts of the body. The liver content of 14C‐labelled cholesterol was very high up to 6 days after the injection. The bile was rapidly intensively labelled, and was regularly regurgitated into the gizzard. The walls of the large arteries contained significantly more 14C‐cholesterol than the surrounding tissues. Organs engaged in steroid hormone synthesis incorporated more yolk cholesterol than “inactive” tissues. In the central nervous system of the youngest chicks, radioactivity was only found to any great extent in the pineal gland, while the peripheral nerves were rapidly intensively labelled. The retention of 14C‐cholesterol after 2 months was greatest in the peripheral nerves and the white matter of the central nervous system. Starvation did not markedly influence the relative distribution pattern of labelled material during the first week after hatching.