Der cytochemische Nachweis von Dehydrogenasen bei Pilzen

Reiss, J

doi:10.1007/bf00416932

Cited by 23 publications

(1 citation statement)

References 51 publications

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“…Glutamate dehydrogenase activity was demonstrated by use of tetrazolium salt in a method based on that of Reiss (1967). The optimum composition of the reaction mixture was determined by experiment and shown, particularly in regard to pH and substrate concentration, to be similar to the optimal reaction mixtures for cell-free extracts (Al-Gharawi and .…”

Section: Enzyme Cytochemistrymentioning

confidence: 99%

CYTOCHEMICAL LOCALIZATION OF GLUTAMATE DEHYDROGENASES DURING CARPOPHORE DEVELOPMENT IN COPRINUS CINEREUS

1979

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SUMMARYThe NAD-and NADP-linked glutamatc dehydrogenases were detected in frozen sections of carpophore tissues by means of tetrazolium salt. Material at all stages of development was examined. From the earliest stages NAD-glutamate dehydrogenase was uniformly distributed in the basidial cells of the hymenium, and as the carpophore developed an increasing proportion of cells of the subhymenium came to express enzyme activity. The NADP-glutamate dehydrogenase (NADP-GDH) was initially limited to basidial cells in isolated patches of the hymenium. As development proceeded the proportion of basidia exhibiting NADP-GDH activity increased, as did the proportion of subhymenial cells showing this enzyme activity. No NADP-GDH activity' was detectable in sections of stipe tissue.

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Section: Enzyme Cytochemistrymentioning

confidence: 99%

CYTOCHEMICAL LOCALIZATION OF GLUTAMATE DEHYDROGENASES DURING CARPOPHORE DEVELOPMENT IN COPRINUS CINEREUS

1979

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Lysosome‐like particles in Geotrichum candidum: A cytochemical study

Reiss

1971

J of Basic Microbiology

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Lysosome-like particles were localized in Geotrichum candidurn by means of the technique for the detection of acid phosphatase of PEARSE and staining with the vital dyes Brilliant cresyl blue and Neutral red. Pretreatment5 of the cells with procedures injuring the lysosome membrane resulted in shortening of the incubation time (2 min instead of 30 min). After exposure to 1% Triton X-100 the cells showed uniform strong staining due to the release of the enzyme into the cytoplasm., Lysosomes are cell particles, surrounded by a single membrane and containing different acid hydrolases. They play a major role in decomposition processes within the cell as well as in various processes of autophagy (DE DUVE 1963, GAHAN 1967. The widespread occurrence of lysosomes in animal cells has been well established (DE DUVE and WATTIAUX 1966). Lysosomes can be localized by different cytochernical procedures. According to GAHAN (1967) a cell organelle can be defined as lysosome when the following prerequisites are fulfilled: a ) the presence of acid hydrolases, b) a single membrane surrounding the organelle, c) the possibility of liberating the hydrolases by suitable methods of destroying the membrane (e.g. hypotonic milieu, detergents), and d ) reaction with vital dyes.Only a few investigations have been conducted to localize lysosomes in fungi. With biochemical methods MATILE and WIEMKEN (1967) GAHAN (1967) and by cytochemical methods and electron microscopy proved the presence of lysosomes in plant pathogens and other fungi. PITT and WALKER (1967) as well as PITT (1968) found that in Botrytis cinerea and other fungi acid phosphatase possesses a particulate localization which led to the conclusion that the granules containing this enzyme may be comparable with animal lysosomes (PITT 1968). To extend our knowledge on the occurrence of lysosomes in fungal cells, a study was undertaken with Geotrichum candidum using cytochemical procedures.

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Lysosome-like particles inGeotrichum candidum: A cytochemical study

Reiss¹

1971

Z Allg Mikrobiol

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Der cytochemische Nachweis von Dehydrogenasen bei Pilzen

Cited by 23 publications

References 51 publications

CYTOCHEMICAL LOCALIZATION OF GLUTAMATE DEHYDROGENASES DURING CARPOPHORE DEVELOPMENT IN COPRINUS CINEREUS

CYTOCHEMICAL LOCALIZATION OF GLUTAMATE DEHYDROGENASES DURING CARPOPHORE DEVELOPMENT IN COPRINUS CINEREUS

Lysosome‐like particles in Geotrichum candidum: A cytochemical study

Lysosome-like particles inGeotrichum candidum: A cytochemical study

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