Depth-enhanced fluorescence imaging using masked detection of structured illumination

Angelo, Joseph; Venugopal, Vivek; Fantoni, Frédéric; Poher, V.; Bigio, Irving J.; Hervé, Lionel; Dinten, Jean‐Marc; Gioux, Sylvain

doi:10.1117/1.jbo.19.11.116008

Cited by 4 publications

(4 citation statements)

References 23 publications

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“…The subtleties of effective system design can require much more complexity. [52][53][54][55][56] The most straightforward configuration is a continuous-wave (CW) system where the source intensity is constant in time. As illustrated in Figure 3 and described in Table 2, the excitation light, typically from a laser diode, a filtered white light source, or a light emitting diode, excites fluorescent molecules from the ground state to a higher energy level.…”

Section: Fluorescence Basicsmentioning

confidence: 99%

“…Fluorescence imaging consists of exciting a contrast agent with appropriate wavelengths of light and detecting the resulting emissions (at different wavelengths of light) by means of a camera and filters. The subtleties of effective system design can require much more complexity 52–56 . The most straightforward configuration is a continuous‐wave (CW) system where the source intensity is constant in time.…”

Section: Introductionmentioning

confidence: 99%

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AAPM Task Group Report 311: Guidance for performance evaluation of fluorescence‐guided surgery systems

Pogue,

Zhu,

Ntziachristos

et al. 2023

Medical Physics

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The last decade has seen a large growth in fluorescence‐guided surgery (FGS) imaging and interventions. With the increasing number of clinical specialties implementing FGS, the range of systems with radically different physical designs, image processing approaches, and performance requirements is expanding. This variety of systems makes it nearly impossible to specify uniform performance goals, yet at the same time, utilization of different devices in new clinical procedures and trials indicates some need for common knowledge bases and a quality assessment paradigm to ensure that effective translation and use occurs. It is feasible to identify key fundamental image quality characteristics and corresponding objective test methods that should be determined such that there are consistent conventions across a variety of FGS devices. This report outlines test methods, tissue simulating phantoms and suggested guidelines, as well as personnel needs and professional knowledge bases that can be established. This report frames the issues with guidance and feedback from related societies and agencies having vested interest in the outcome, coming from an independent scientific group formed from academics and international federal agencies for the establishment of these professional guidelines.

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Section: Fluorescence Basicsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

AAPM Task Group Report 311: Guidance for performance evaluation of fluorescence‐guided surgery systems

Pogue,

Zhu,

Ntziachristos

et al. 2023

Medical Physics

Self Cite

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“…Imaging with increased spatial frequency decreases the average collected photon path length, resulting in a decreased average photon penetration depth 21 , 22 , 33 and increased sensitivity to shorter path length interactions 41 , 98 . The spatial pattern can be adjusted during fluorescence imaging to vary the depth and contrast, 33 , 99 , 100 or pushed high such that sensitivity to absorption is lost [cf. Fig.…”

Section: Spatial Frequency-domain Imagingmentioning

confidence: 99%

Review of structured light in diffuse optical imaging

et al. 2018

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Diffuse optical imaging probes deep living tissue enabling structural, functional, metabolic, and molecular imaging. Recently, due to the availability of spatial light modulators, wide-field quantitative diffuse optical techniques have been implemented, which benefit greatly from structured light methodologies. Such implementations facilitate the quantification and characterization of depth-resolved optical and physiological properties of thick and deep tissue at fast acquisition speeds. We summarize the current state of work and applications in the three main techniques leveraging structured light: spatial frequency-domain imaging, optical tomography, and single-pixel imaging. The theory, measurement, and analysis of spatial frequency-domain imaging are described. Then, advanced theories, processing, and imaging systems are summarized. Preclinical and clinical applications on physiological measurements for guidance and diagnosis are summarized. General theory and method development of tomographic approaches as well as applications including fluorescence molecular tomography are introduced. Lastly, recent developments of single-pixel imaging methodologies and applications are reviewed.

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“…22 Further segmentation would require tomographic reconstruction methods. [22][23][24] The use of striped patterns of different frequencies allows for a clear delineation between the tumor and the background fluorescence. In doing so, tumor contrast enhancement does not rely on arbitrary, user-defined thresholding, 2 and the entirety of the fluorescence information remains visible to the operator, thus preserving detection sensitivity.…”

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confidence: 99%

Enhancingin vivotumor boundary delineation with structured illumination fluorescence molecular imaging and spatial gradient mapping

et al. 2016

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Fluorescence imaging, in combination with tumor-avid near-infrared (NIR) fluorescent molecular probes, provides high specificity and sensitivity for cancer detection in preclinical animal models, and more recently, assistance during oncologic surgery. However, conventional camera-based fluorescence imaging techniques are heavily surface-weighted such that surface reflection from skin or other nontumor tissue and nonspecific fluorescence signals dominate, obscuring true cancer-specific signals and blurring tumor boundaries. To address this challenge, we applied structured illumination fluorescence molecular imaging (SIFMI) in live animals for automated subtraction of nonspecific surface signals to better delineate accumulation of an NIR fluorescent probe targeting α4β1 integrin in mice bearing subcutaneous plasma cell xenografts. SIFMI demonstrated a fivefold improvement in tumor-to-background contrast when compared with other full-field fluorescence imaging methods and required significantly reduced scanning time compared with diffuse optical spectroscopy imaging. Furthermore, the spatial gradient mapping enhanced highlighting of tumor boundaries. Through the relatively simple hardware and software modifications described, SIFMI can be integrated with clinical fluorescence imaging systems, enhancing intraoperative tumor boundary delineation from the uninvolved tissue.

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Depth-enhanced fluorescence imaging using masked detection of structured illumination

Cited by 4 publications

References 23 publications

AAPM Task Group Report 311: Guidance for performance evaluation of fluorescence‐guided surgery systems

AAPM Task Group Report 311: Guidance for performance evaluation of fluorescence‐guided surgery systems

Review of structured light in diffuse optical imaging

Enhancingin vivotumor boundary delineation with structured illumination fluorescence molecular imaging and spatial gradient mapping

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