Depth distribution of euryhaline halophilic bacteria in Suribati Ike, a meromictic lake in East Antarctica

Naganuma, Takeshi; Hua, Phuc Ngoc; Okamoto, Tatsuya; Ban, Syuhei; Imura, Satoru; Kanda, Hiroshi

doi:10.1007/s00300-005-0026-0

Cited by 18 publications

(5 citation statements)

References 34 publications

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“…It was remarkable that a number of halotolerant/halophilic groups belonging to Bacillus, Halobacillus, Staphylococcus and Halomonas found in this study, were previously found in saline environments such as the Salt Plains National Wildlife Refuge, Great Salt Plains of Oklahoma [22], Bolivian hypersaline lake [23], deep-sea sediments [24][25], and tropical marine sediments [26]. These bacterial groups were found both in the river water and groundwater samples.…”

Section: Salt Tolerance and Requirements Of Unique Isolatessupporting

confidence: 54%

Halophilic and Halotolerant Bacteria from River Waters and Shallow Groundwater Along the Rouge River of Southeastern Michigan

Tiquia

Davis²,

Hadid³

et al. 2007

Environmental Technology

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The use of sodium chloride to melt highway and road snow is believed to have a significant effect on the groundwater ecosystem of the rivers where the salt from the roads drain. As the river composition changes, the bacterial population also changes to favour those bacteria that are more suited to the higher salt concentrations. In this experiment, we surveyed the cultivable salt-loving organisms (halophiles) on three sites that encompass the Rouge River (Lotz; site 1, Lilly, site; 8, and Ford Field, site 9). A total of 125 isolates were surveyed. Representative isolates of distinct morphologies were subjected to physiological test, using API strips and identified by 16 rDNA sequence analysis. The 16S rDNA sequences were analyzed and compared with sequences from Genbank. Results indicated that the SSU rRNA sequences of the bacterial isolates were similar to six major genera, Bacillus, Staphylococcus, Halobacillus, Paenabacillus, Halomonas, and Clostridium. Half of the isolates sequenced were similar to Bacillus spp. The API assay showed that the majority of the isolates were positive for the enzymes tryptophane deaminase, gelatinase and beta-galactosidase. Indole production, acetoin production and citrate utilization were not observed for any isolates. Fermentation of carbohydrates was observed for very few isolates. The primary enzyme found in all isolates was arginine dihydrolase, which might be an indicator of the presence of such enzyme in halophilic and halotolerant bacteria present in the Rouge River.

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Section: Salt Tolerance and Requirements Of Unique Isolatessupporting

confidence: 54%

Halophilic and Halotolerant Bacteria from River Waters and Shallow Groundwater Along the Rouge River of Southeastern Michigan

Tiquia

Davis²,

Hadid³

et al. 2007

Environmental Technology

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“…Both are typical saline lakes of Type 4. Naganuma et al [2005] reported a vertical distribution of salinity and halophilic bacteria in the water column of Lake Suribachi Ike and high water temperature (about 15°C) near the lake floor. No large bubbles were observed in the ice, which was semiopaque because of brine formation like sea ice, at Lake Suribachi Ike and Nezumi Ike.…”

Section: Resultsmentioning

confidence: 99%

Methane efflux from bubbles suspended in ice‐covered lakes in Syowa Oasis, East Antarctica

et al. 2009

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[1] This is the first estimation of methane efflux from bubbles in lake ice in Antarctica. Bubbles suspended in shallow ice in 20 lakes were observed as part of the operations of the 45th Japanese Antarctic Research Expedition in ice-free rocky areas along the eastern coast of Lützow-Holm Bay (Syowa Oasis) in East Antarctica in 2004. Anomalous methane concentrations in bubbles suspended in lake ice and anomalous dissolved methane concentrations in lake water were frequently found. Methane concentrations in bubbles varied of 5 orders of magnitude, from 1.9 ppmv to 18% vol/vol. A procedure that makes estimations of methane flux from ice bubbles possible has been developed, on the basis of a relationship between bubble-density distribution, brightness observed by digital photographs, and methane concentration in bubbles. Such a procedure applied to Lake Oyako Ike in the Skarvsnes area, where the maximum methane concentration was observed, suggests that total bubble volume is about 0.6% of ice volume and the mass of methane in bubbles in the lake is about 74 kg. Almost all gases in bubbles are released to the atmosphere in the early ice melt season (a period of a few weeks in December and January). By applying area fraction frequency distributions to methane concentration ranges for 20 lakes surveyed, extrapolation to the whole Syowa Oasis, including 110 lakes, would give a provisional estimate of total emission of about 2 tons-CH 4 /yr.

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“…As might have been anticipated (Shahjee et al ., ), a number of the genera recovered from the brine and alkali lakes have been previously associated with osmoprotectants or hyperosmotic environments, including Acinetobacter , Arthrobacter , Bacillus , Halomonas , Idiomarina , and Marinobacter , Nesterenkonia , and Paenibacillus (e.g. Mogilnaya et al ., ; Naganuma et al ., ). Isolates derived from the brine‐ and alkali‐lake samples were five‐ and four‐fold, respectively, more likely than isolates derived from freshwater lakes, to have an osmolyte content that was statistically greater than the E .…”

Section: Discussionmentioning

confidence: 97%

Cross-tolerance between osmotic and freeze-thaw stress in microbial assemblages from temperate lakes

Wilson

Frazer

Cumming

et al. 2012

FEMS Microbiol Ecol

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Osmotic stress can accompany increases in solute concentrations because of freezing or high-salt environments. Consequently, microorganisms from environments with a high-osmotic potential may exhibit cross-tolerance to freeze stress. To test this hypothesis, enrichments derived from the sediment and water of temperate lakes with a range of salt concentrations were subjected to multiple freeze-thaw cycles. Surviving isolates were identified and metagenomes were sampled prior to and following selection. Enrichments from alkali lakes were typically the most freeze-thaw resistant with only 100-fold losses in cell viability, and those from freshwater lakes were most susceptible, with cell numbers reduced at least 100,000-fold. Metagenomic analysis suggested that selection reduced assemblage diversity more in freshwater samples than in those from saline lakes. Survivors included known psychro-, halo- and alkali-tolerant bacteria. Characterization of freeze-thaw-resistant isolates from brine and alkali lakes showed that few isolates had ice-associating activities such as antifreeze or ice nucleation properties. However, all brine- and alkali-derived isolates had high intracellular levels of osmolytes and/or appeared more likely to form biofilms. Conversely, these phenotypes were infrequent amongst the freshwater-derived isolates. These observations are consistent with microbial cross-tolerance between osmotic and freeze-thaw stresses.

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Depth distribution of euryhaline halophilic bacteria in Suribati Ike, a meromictic lake in East Antarctica

Cited by 18 publications

References 34 publications

Halophilic and Halotolerant Bacteria from River Waters and Shallow Groundwater Along the Rouge River of Southeastern Michigan

Halophilic and Halotolerant Bacteria from River Waters and Shallow Groundwater Along the Rouge River of Southeastern Michigan

Methane efflux from bubbles suspended in ice‐covered lakes in Syowa Oasis, East Antarctica

Cross-tolerance between osmotic and freeze-thaw stress in microbial assemblages from temperate lakes

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