Deproteinization of Polysaccharide from the Stigma Maydis by Sevag Method

Li, Xia; Zhao, Rui; Zhou, Hong Li; Wu, De Heng

doi:10.4028/www.scientific.net/amr.340.416

Cited by 37 publications

(21 citation statements)

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“…To remove proteins, proteinase K and n ‐butyl alcohol (5 : 1, 71‐36‐3; BOC Sciences, New York, NY, USA) were used as described previously (Li et al . ). The aqueous layer was collected followed by dialysis with distilled water overnight.…”

Section: Methodsmentioning

confidence: 97%

Antibiofilm activity of threeActinomycetestrains againstStaphylococcus epidermidis

Xie

Zeng

Ren

et al. 2018

Lett Appl Microbiol

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Significance and Impact of the Study: Staphylococcus epidermidis infections are frequently associated with biofilms that are difficult to eradicate with conventional antibiotics. The new biofilm inhibitors from Actinomycete will have a great value in the prevention and treatment of dairy cow mastitis and other biofilm-related infections. AbstractThis study sought to identify novel and nontoxic biofilm inhibitors from the Actinomycete library for attenuating biofilm formation by Staphylococcus epidermidis. After investigating the antibiofilm activities of spent media from 185 Actinomycete strains using two S. epidermidis strains (ATCC 35984 and a clinical strain 5-121-2) as target bacteria, three strains of tested Actinomycete (TRM 46200, TRM 41337, and TRM 46814) showed a significant inhibition against S. epidermidis biofilm formation without affecting the growth of planktonic cells. The characteristics of three strains of supernatants suggested that hydrophilic compound possibly extracellular peptides or proteins from these three strains, confer the biofilm reduction in S. epidermidis. An attempt was made to assess their effects on biofilm components and cell surface hydrophobicities in order to disclose acting mechanisms. The crude proteins from spent media of three strains degraded not only exopolysaccharides but also extracellular DNA in S. epidermidis biofilm. The active substances in crude proteins caused S. epidermidis cells to become less hydrophobic. Given these results, the metabolites from Actinomycete strains should keep further attention as potential antibiofilm agents against biofilm formation of S. epidermidis, even biofilm infections of the other bacteria.

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Section: Methodsmentioning

confidence: 97%

Antibiofilm activity of threeActinomycetestrains againstStaphylococcus epidermidis

Xie

Zeng

Ren

et al. 2018

Lett Appl Microbiol

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show abstract

“…Exopolysaccharides (EPS) in the S. epidermidis culture was collected by water extraction and alcohol precipitation (Jin and Zhao ; Xie et al ). To remove proteins, proteinase K and n ‐butyl alcohol (5 : 1, BOC Sciences, 71‐36‐3) were used as described previously (Li et al ). The aqueous layer was collected followed by dialysis with distilled water overnight.…”

Section: Methodsmentioning

confidence: 99%

Streptomyces ‐derived actinomycin D inhibits biofilm formation via downregulating ica locus and decreasing production of PIA in Staphylococcus epidermidis

Xie

Zeng

et al. 2019

J Appl Microbiol

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Aim The objective of this study was to investigate the biofilm inhibitory activity of Streptomyces‐derived actinomycin D against biofilm formation by Staphylococcus epidermidis. Methods and Results The microtitre plate method and microscopy were used to detect the biofilm formation of S. epidermidis. And an attempt was made to detect the effect of actinomycin D on important biofilm components, exopolysaccharides (EPS) in S. epidermidis using precolumn derivation HPLC. Also cell surface hydrophobicities of S. epidermidis were assessed to explore action mechanisms. The qPCR was performed to demonstrate the genetic mechanisms of biofilm formation by S. epidermidis. Unlike other antibiotics, actinomycin D (1·5 μg ml−1) from Streptomyces luteus significantly inhibited biofilm formation by S. epidermidis. Additionally, it effectively inhibited S. epidermidis cells from adhering to glass slides. Actinomycin D downregulated ica locus and then the reduced polysaccharide intercellular adhesin production caused S. epidermidis cells to become less hydrophobic, thus supporting its anti‐biofilm effect. Conclusion Streptomyces‐derived actinomycin D is active in inhibiting the biofilm formation of S. epidermidis. Significance and Impact of the Study Actinomycin D can be used as a promising antibiofilm agent in inhibiting S. epidermidis biofilm formation. The study is also the first insight into how actinomycin D inhibited the biofilm formation of S. epidermidis. Actinomycin D could potentially be used to reduce the risk of biofilm‐associated infections. Our study also suggests that the metabolites from Actinomycete strains keep further attention as potential antibiofilm agents against biofilm formation of S. epidermidis, even biofilm infections of the other bacteria.

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“…The above was performed three times, and the supernatants were combined, concentrated in a rotary evaporator under a reduced pressure, and then filtered. The concentrated solution was deproteinated by the Sevag method [ 53 ], trichloroacetic acid method [ 54 ], and hydrochloric acid method [ 55 ], with each method repeated six times. The solution was decolorized by the macroporous resin separation method.…”

Section: Methodsmentioning

confidence: 99%

Isolation, Purification and Structural Characterization of Two Novel Water-Soluble Polysaccharides from Anredera cordifolia

et al. 2017

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Anredera cordifolia, a climber and member of the Basellaceae family, has long been a traditional medicine used for the treatment of hyperglycemia in China. Two water-soluble polysaccharides, ACP1-1 and ACP2-1, were isolated from A. cordifolia seeds by hot water extraction. The two fractions, ACP1-1 and ACP2-1 with molecular weights of 46.78 kDa ± 0.03 and 586.8 kDa ± 0.05, respectively, were purified by chromatography. ACP1-1 contained mannose, glucose, galactose in a molar ratio of 1.08:4.65:1.75, whereas ACP2-1 contained arabinose, ribose, galactose, glucose, mannose in a molar ratio of 0.9:0.4:0.5:1.2:0.9. Based on methylation analysis, ultraviolet and Fourier transform-infrared spectroscopy, and periodate oxidation the main backbone chain of ACP1-1 contained (1→3,6)-galacturonopyranosyl residues interspersed with (1→4)-residues and (1→3)-mannopyranosyl residues. The main backbone chain of ACP2-1 contained (1→3)-galacturonopyranosyl residues interspersed with (1→4)-glucopyranosyl residues.

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Deproteinization of Polysaccharide from the Stigma Maydis by Sevag Method

Cited by 37 publications

References 0 publications

Antibiofilm activity of threeActinomycetestrains againstStaphylococcus epidermidis

Antibiofilm activity of threeActinomycetestrains againstStaphylococcus epidermidis

Streptomyces ‐derived actinomycin D inhibits biofilm formation via downregulating ica locus and decreasing production of PIA in Staphylococcus epidermidis

Isolation, Purification and Structural Characterization of Two Novel Water-Soluble Polysaccharides from Anredera cordifolia

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