Deproteinated potato wastewater as a low-cost nitrogen substrate for very high yeast biomass quantities: starting point for scaled-up applications

Abstract

The aim of this research was to investigate the influence of deproteinated potato wastewater (DPW) supplemented by different initial concentrations of glucose on biomass yield, especially lipids biosynthesis and fatty acids composition. DPW is a low-cost source of nitrogen and other biogenic elements. It allowed for an efficient yeast cells growth and a very high biomass yield of Rhodotorula glutinis var. rubescens LOCKR13. A short time of incubation leads to a biomass rich in protein, which can be a source of single-cell protein. The strain was able to accumulate 46% cell dry weight (CDW) of lipids in control medium and only 18% when DPW medium were used. The maximum volumetric lipids efficiency in the DPW medium was 6.14 g L −1. Oleic (41.0-51.5%), palmitic (15.3-17.8%), and stearic acids (5.8-7.5%) dominated the entire pool of fatty acids. Theoretical calculations indicated a possibility to use the methylated esters of lipids from R. glutinis var. rubescens yeast as a biodiesel.

The Thermal Properties and Nutritional Value of Biomass of Oleaginous Yeast Rhodotorula sp. during Glucose Fed-Batch Cultivation in Medium with Waste Nitrogen

“…The composition of fatty acids influences the intended use, so if the goal is to produce biodiesel, there should be a high proportion of SFAs. Then the final product has appropriate properties, such as cetane number (CN), flash point (FP), and viscosity (µ) [8]. If SCO has a nutritionally proper fatty acid content, it can be used for food production [9].…”

“…Previous research showed that strain Rhodotorula glutinis var. rubescens can grow efficiently (biomass yield 39.52 g L −1 ) in deproteinated potato wastewater and glucose medium [8], and after fed-batch cultivation, the content of lipids in biomass exceed >34% d.w. [10]. Except for accumulated fat in intracellular lipid droplets, the cells are also rich in proteins, carbohydrates, and carotenoids [11].…”

Enhancing Red Yeast Biomass Yield and Lipid Biosynthesis by Using Waste Nitrogen Source by Glucose Fed-Batch at Low Temperature

“…Deproteinated potato wastewater (DPW) is a liquid waste of the starch industry (over 12 million m 3 /year) [8], which can be a source of nitrogen in the media used for yeast biomass growth and the biosynthesis of microbial oils [23]. The high productivity depends not only on the possibilities of the strain to accumulate lipids in lipid bodies but also on biomass yield [20].…”