Depressive symptoms in obesity: Relative contribution of low-grade inflammation and metabolic health

Delgado, Inês; Huet, Lison; Dexpert, Sandra; Beau, Cédric; Forestier, Damien; Ledaguenel, Patrick; Aubert, Agnès; Sauvant, Julie; Aouizerate, Bruno; Magne, Eric; Capuron, Lucile

doi:10.1016/j.psyneuen.2018.02.030

Cited by 51 publications

(37 citation statements)

References 46 publications

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“…These data are in line with data produced previously (Zaitone and Essawy, 2012; Tunapong et al, 2018). In agreement, human studies highlighted a causal relationship between excessive caloric intake, weight gain, and tissue resistance to insulin (Delgado et al, 2018). Furthermore, a plethora of data reports the interplay of pro-inflammatory cytokines and adipokines in the pathology of insulin resistance and associating comorbidities (Hotamisligil et al, 1993; Gregor and Hotamisligil, 2011; Alcalá et al, 2017).…”

Section: Discussionmentioning

confidence: 78%

Pharmacological Action of a Pregnane Glycoside, Russelioside B, in Dietary Obese Rats: Impact on Weight Gain and Energy Expenditure

et al. 2018

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Background and purpose: Russelioside B (RB) is a pregnane glycoside obtained from Caralluma quadrangula; a herb with antidiabetic, anti-inflammatory, and antihyperlipidemic activities. The present experiment tested the possible role of RB in controlling weight gain in rats fed on high fat (HF) diet.Methods: RB was separated from the n-butanol fraction of the crude methanolic extract by chromatographic separation on a Si gel column according to the procedures described previously. The experiment of the biological assessment of RB used 32 male Wistar rats (4 groups, n = 8). Group 1 rats were fed with a palatable normal diet. Group 2, 3, and 4 were fed on HF diet for 16 weeks. Group 2 served as the HF diet control group while Group 3 and 4 received daily oral doses of RB (25 and 50 mg/kg) during the last four weeks. Animals’ parameters like weight gain, fasting level of blood sugar, serum lipids, and serum liver enzyme activities were measured. Liver or adipose tissue weight was divided by the rat’s body weight and multiplied by 100 to obtain the liver or adipose tissue index, respectively. Adipose tissues were processed for histopathological examination, measurement of mRNA expression of visfatin, leptin, adiponectin, uncoupling protein-1 (UCP-1), and carnitine palmitoyl transferase-1 (CPT-1). Furthermore, serum levels of insulin, interleukin-6 (IL-6), IL-1β, tumor necrosis factor-α (TNF-α), leptin, resistin, and adiponectin were assessed using ELISA kits.Results: Rats fed with the HF diet exhibited significant body weight gain, abnormal liver function, disturbed lipid profile, and greater serum level of pro-inflammatory cytokines in addition to greater insulin resistance, adipose tissue and liver indices. Further, rats fed with the HF diet displayed upregulations in the expression of visfatin and leptin with downregulations in the expression of adiponectin, UCP-1, and CPT-1 compared to normal rats. Interestingly, RB (25 or 50 mg/kg) favorably modulated the measured parameters.Conclusion: Data from this study documented the beneficial role of RB in diminishing weight gain, improving the inflammatory perturbations and energy expenditure in HF diet fed rats. Therefore, RB might be a promising candidate for obesity.

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Section: Discussionmentioning

confidence: 78%

Pharmacological Action of a Pregnane Glycoside, Russelioside B, in Dietary Obese Rats: Impact on Weight Gain and Energy Expenditure

et al. 2018

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“…In the USA alone, a morbid BMI is responsible for approximately 18% of all deaths in the age group of 40 to 85 year-olds [4]. Lastly, prolonged caloric excess leads to adipose tissue remodeling and thereby to chronic inflammation including type 2 diabetes [5,6], cardiovascular diseases [7,8], cancer [9,10], osteoporosis [11], chronic kidney disease [12] as well as depression [13], which altogether constitute the leading causes of disability and death in developed countries.…”

Section: Introductionmentioning

confidence: 99%

Examination of ex-vivo viability of human adipose tissue slice culture

et al. 2020

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Obesity is associated with significantly higher mortality rates, and excess adipose tissue is involved in respective pathologies. Here we established a human adipose tissue slice cultures (HATSC) model ex vivo. HATSC match the in vivo cell composition of human adipose tissue with, among others, mature adipocytes, mesenchymal stem cells as well as stroma tissue and immune cells. This is a new method, optimized for live imaging, to study adipose tissue and cell-based mechanisms of obesity in particular. HATSC survival was tested by means of conventional and immunofluorescence histological techniques, functional analyses and live imaging. Surgery-derived tissue was cut with a tissue chopper in 500 μm sections and transferred onto membranes building an air-liquid interface. HATSC were cultured in six-well plates filled with Dulbecco's Modified Eagle's Medium (DMEM), insulin, transferrin, and selenium, both with and without serum. After 0, 1, 7 and 14 days in vitro, slices were fixated and analyzed by morphology and Perilipin A for tissue viability. Immunofluorescent staining against IBA1, CD68 and Ki67 was performed to determine macrophage survival and proliferation. These experiments showed preservation of adipose tissue as well as survival and proliferation of monocytes and stroma tissue for at least 14 days in vitro even in the absence of serum. The physiological capabilities of adipocytes were functionally tested by insulin stimulation and measurement of Phospho-Akt on day 7 and 14 in vitro. Viability was further confirmed by live imaging using Calcein-AM (viable cells) and propidium iodide (apoptosis/necrosis). In conclusion, HATSC have been successfully established by preserving the monovacuolar form of adipocytes and surrounding macrophages and connective tissue. This model allows further analysis of mature human adipose tissue biology ex vivo.

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“…Similarly, inflammation continues to have tremendous promise as a common mechanism by which psychological experience affects mental, physical (e.g. Dantzer et al , 2008 ; Lutgendorf and Sood, 2011 ; Delgado et al , 2018 ) and social health ( Eisenberger et al , 2017 ; Nusslock and Miller, 2016 ). Maintaining health neuroscience’s original focus on physical health while accounting for other influential dimensions of health will be important for reaching a consensus about shared mechanisms, points of intervention, and for ultimately gaining a holistic understanding of health.…”

Section: New and Continuing Emphases: Where Can The Scan Community Comentioning

confidence: 99%

Health neuroscience 2.0: integration with social, cognitive and affective neuroscience

Inagaki

2020

Social Cognitive and Affective Neuroscience

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Depressive symptoms in obesity: Relative contribution of low-grade inflammation and metabolic health

Cited by 51 publications

References 46 publications

Pharmacological Action of a Pregnane Glycoside, Russelioside B, in Dietary Obese Rats: Impact on Weight Gain and Energy Expenditure

Pharmacological Action of a Pregnane Glycoside, Russelioside B, in Dietary Obese Rats: Impact on Weight Gain and Energy Expenditure

Examination of ex-vivo viability of human adipose tissue slice culture

Health neuroscience 2.0: integration with social, cognitive and affective neuroscience

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