Objective: To determine the prevalence of depression in patients undergoing hemodialysis. Study Design: Cross-sectional study. Place and Duration: Institute of Kidney Diseases Hayatabad, Peshawar and PAF Hospital Islamabad for the duration from December 2021 to May 2022. Methodology: Two hundred and twenty patients on hemodialysis of either gender with ages 25-70 years were enrolled. Detailed demographics were recorded after taking informed written consent. Patients' Health Questionnaire-9 (PHQ-9) questions were used to determine the frequency of depression. Severity of depression was also examined. Data was analyzed by SPSS 24.0. Results: There were 135 (61.36%) males while 85 (38.64%) patients were females. Mean age of patients was 46.85±12.38 years. Mean time duration of hemodialysis was 5.25±2.46 years. Depression was found in 160 (72.73%) patients, among them 30 (13.64%) patients had mild, 75 (34.09%) had moderate and 55 (25%) had severe depression. Patients on hemodialysis less than 5 years found more depressive as compared to >5 years. (p-value <0.05). Conclusion: It is concluded that the prevalence of depression in patients of hemodialysis was high.